Complete Genome Sequence Analysis of Pandoraea pnomenusa Type Strain DSM 16536T Isolated from a Cystic Fibrosis Patient

Lim, Yan-Lue; Ee, Robson; Yong, Delicia; Yu, Choo Yee; Ang, Geik Yong; Tee, Kok Keng; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.3389/fmicb.2016.00109

Cited by 9 publications

(9 citation statements)

References 36 publications

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“…Major advances in our understanding of the role(s) of Pandoraea in disease progression in CF rest upon knowing the virulence properties of these organisms. In this context, the whole genome sequences for P. pnomenusa generated by our research group (Ee et al, 2015 ) and others (Lim et al, 2016 ) provide a valuable resource, that could be easily mined by researchers interested in forming a better understanding of Pandoraea colonization in people with CF and discovering potential therapeutic targets. In addition, our results demonstrating that P. pnomenusa is a facultative anaerobe are significant.…”

Section: Discussionmentioning

confidence: 99%

Pandoraea pnomenusa Isolated from an Australian Patient with Cystic Fibrosis

et al. 2016

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Pandoraea species are considered as emerging pathogens in people with cystic fibrosis (CF). The contribution of these organisms to disease progression in CF patients is not fully understood owing in large measure to the scant reports in clinical and research literature describing their colonization of CF patients and their associated virulence determinants. In an effort to increase awareness and evidence for Pandoraea spp. infection in people with CF, and to stimulate research aimed at unraveling the pathogenic properties of Pandoraea, we report a case of a 26-year-old Australian (Tasmanian) man with CF who was chronically infected with Pandoraea pnomenusa for at least one year prior to his death from respiratory failure. In addition, we describe for the first time evidence suggesting that this bacterium is a facultative anaerobe and report on the availability of a whole genome sequence for this organism. To the best of our knowledge, this report represents only the second clinical case study of P. pnomenusa infection in the world, and the first in an Australian CF patient.

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Section: Discussionmentioning

confidence: 99%

Pandoraea pnomenusa Isolated from an Australian Patient with Cystic Fibrosis

et al. 2016

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“…The clinical species were mainly isolated from respiratory specimens of cystic fibrosis (CF) patients [1, 3, 7, 9–24]. To date, cases of Pandoraea species caused colonization or infection have been reported all over the world, including USA [1, 7, 12, 25], Denmark [1, 10, 26], Germany [13], France [18, 19], Ireland [26], Argentina [20, 24], Spain [16, 17, 27, 28], Australia [3, 14, 15], Canada [1], UK [1, 9, 22], China [29], Belgium [1], Brazil [1], Sweden [1] (See at Fig. 3).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular biotechnology has been used to analyze virulence genes and drug resistance genes of Pandoraea species. Lim et al [22] sequenced the complete genome of a Pandoraea pnomenusa strain and identified 16 virulence factors, which are well-characterized virulence determinants in some other pathogens. Robson et al [38] sequenced the complete genome of two Pandoraea pnomenusa strains and found 130 gene sequences related to virulence, disease and drug resistance.…”

Section: Discussionmentioning

confidence: 99%

Pneumonia due to Pandoraea Apista after evacuation of traumatic intracranial hematomas:a case report and literature review

Lin

Luo

et al. 2019

BMC Infect Dis

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BackgroundPandoraea species is a newly described genus, which is multidrug resistant and difficult to identify. Clinical isolates are mostly cultured from cystic fibrosis (CF) patients. CF is a rare disease in China, which makes Pandoraea a total stranger to Chinese physicians. Pandoraea genus is reported as an emerging pathogen in CF patients in most cases. However, there are few pieces of evidence that confirm Pandoraea can be more virulent in non-CF patients. The pathogenicity of Pandoraea genus is poorly understood, as well as its treatment. The incidence of Pandoraea induced infection in non-CF patients may be underestimated and it’s important to identify and understand these organisms.Case presentationWe report a 44-years-old man who suffered from pneumonia and died eventually. Before his condition deteriorated, a Gram-negative bacilli was cultured from his sputum and identified as Pandoraea Apista by matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS).ConclusionPandoraea spp. is an emerging opportunistic pathogen. The incidences of Pandoraea related infection in non-CF patients may be underestimated due to the difficulty of identification. All strains of Pandoraea show multi-drug resistance and highly variable susceptibility. To better treatment, species-level identification and antibiotic susceptibility test are necessary.

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“…Previously identified Pandoraea spp. are predominantly clinical isolates with a few strains being associated with biodegradation of environmental pollutants (Lim et al, 2016). For example, Pandoraea participates in the bioremediation of lignin, plasticizer dibutyl phthalate, phenol and petroleum hydrocarbon pollutants (Sarkar et al, 2017; Fang et al, 2018; Liu et al, 2018; Yang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Whole Genome Sequencing and Comparative Genomics Analyses of Pandoraea sp. XY-2, a New Species Capable of Biodegrade Tetracycline

et al. 2019

Front. Microbiol.

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Few bacteria are resistant to tetracycline and can even biodegrade tetracycline in the environment. In this study, we isolated a bacterium Pandoraea sp. XY-2, which could biodegrade 74% tetracycline at pH 7.0 and 30°C within 6 days. Thereafter, we determined the whole genome sequence of Pandoraea sp. XY-2 genome is a single circular chromosome of 5.06 Mb in size. Genomic annotation showed that two AA6 family members-encoding genes and nine glutathione S-transferase (GSTs)-encoding genes could be relevant to tetracycline biodegradation. In addition, the average nucleotide identities (ANI) analysis between the genomes of Pandoraea sp. XY-2 and other Pandoraea spp. revealed that Pandoraea sp. XY-2 belongs to a new species. Moreover, comparative genome analysis of 36 Pandoraea strains identified the pan and specific genes, numerous single nucleotide polymorphisms (SNPs), insertions, and deletion variations (InDels) and different syntenial relationships in the genome of Pandoraea sp. XY-2. Finally, the evolution and the origin analysis of genes related to tetracycline resistance revealed that the six tetA(48) genes and two specificgenes tetG and tetR in Pandoraea sp. XY-2 were acquired by horizontal gene transfer (HGT) events from sources related to Paraburkholderia, Burkholderia, Caballeronia, Salmonella, Vibrio, Proteobacteria, Pseudomonas, Acinetobacter, Flavimaricola, and some unidentified sources. As a new species, Pandoraea sp. XY-2 will be an excellent resource for the bioremediation of tetracycline-contaminated environment.

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Complete Genome Sequence Analysis of Pandoraea pnomenusa Type Strain DSM 16536T Isolated from a Cystic Fibrosis Patient

Cited by 9 publications

References 36 publications

Pandoraea pnomenusa Isolated from an Australian Patient with Cystic Fibrosis

Pandoraea pnomenusa Isolated from an Australian Patient with Cystic Fibrosis

Pneumonia due to Pandoraea Apista after evacuation of traumatic intracranial hematomas:a case report and literature review

Whole Genome Sequencing and Comparative Genomics Analyses of Pandoraea sp. XY-2, a New Species Capable of Biodegrade Tetracycline

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