Complete genome sequence analysis of novel human bocavirus reveals genetic recombination between human bocavirus 2 and human bocavirus 4

Khamrin, Pattara; Okitsu, Shoko; Ushijima, Hiroshi; Maneekarn, Niwat

doi:10.1016/j.meegid.2013.03.040

Cited by 22 publications

(18 citation statements)

References 27 publications

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“…As for the HBoV4 strain CMH-S011-11 (Thailand), its identity to Novosibirsk isolates according to the complete nucleotide sequences is 95%. This Thailand strain displays high similarity to the Novosibirsk isolates in its ORF3 sequence (98-99%); however, analysis of the remaining part of its genome revealed strong similarity to the HBoV2 KU1 isolate, thereby suggesting some recombination events in its evolutionary history (Khamrin et al, 2013). Similar results have been obtained with the trees constructed for the amino acid sequences of the proteins of the studied HBoV strains.…”

Section: Sequencing and Analysis Of Hbov4 Genomessupporting

confidence: 76%

“…1). However, this Thailand strain originated from recombination with HBoV2 (Khamrin et al, 2013), whereas no significant recombination events have been detected in the Novosibirsk HBoV4 isolates.…”

Section: Discussionmentioning

confidence: 85%

“…Currently, only two complete genome sequences of HBoV4 strains-from Nigeria (2007) (Kapoor et al, 2010) and Thailand (2011) (Khamrin et al, 2013)-are available in the databases.…”

Section: Sequencing and Analysis Of Hbov4 Genomesmentioning

confidence: 99%

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A study of the human bocavirus replicative genome structures

et al. 2015

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Section: Sequencing and Analysis Of Hbov4 Genomessupporting

confidence: 76%

Section: Discussionmentioning

confidence: 85%

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A study of the human bocavirus replicative genome structures

et al. 2015

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“…The VP gene of parvoviruses encodes a capsid protein that is essential for viral absorption, entry into host cells and replication and for production of infectious virus (22). Recombination events within the VP gene have been reported in several parvovirus species, such as between Muscovy duck parvovirus and goose parvovirus (23), between human bocavirus 2 and human bocavirus 4 (24), and within bufaviruses (11). However, the influences of these recombination events on viral pathogenicity have not been investigated.…”

Section: Discussionmentioning

confidence: 99%

Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains

et al. 2020

Front. Vet. Sci.

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Equine parvovirus-hepatitis (EqPV-H) was first reported in a horse that died of equine serum hepatitis in the USA in 2018, and was determined having a strong association with equine serum hepatitis in the following studies. As a newly discovered virus, the genomic sequences of only seven EqPV-H strains have been reported. Considering this, an epidemiological study was performed to investigate the prevalence of EqPV-H in equines in Guangdong Province in China, and obtain genomic sequences of the field prevalent EqPV-H strains. The detection rate of EqPV-H was finally determined to be 8.33% (95% CI: 2.8-18.4%), and EqPV-H's coinfection with equine hepacivirus and equine pegivirus was also determined. Then, the genomes of the Chinese field EqPV-H strains were obtained by PCR, sequencing, and assembly. Through bootscanning analysis, Simplot analysis, and phylogenetic analysis, strong evidence for natural recombination events were found in two Chinese field EqPV-H strains. The natural recombination events occurred between the Chinese and American strains, and were determined within VP protein. Finally, the genetic distance of EqPV-H strains was investigated. Nucleotide identities of 97.1-99.9% and 95.2-100% were found for NS and VP between EqPV-H strains, respectively. Together with other molecular evidence obtained in the present study, the genetic diversity of EqPV-H was determined. Taken together, the results of the present study expand our knowledge on the epidemiological characteristics, genetic variability, and evolution of EqPV-H.

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“…HBoV1 is most commonly detected from respiratory specimens, while HBoV2-4 is often detected in fecal specimens [4][5][6]10]. Furthermore, previous literature has shown that HBoVs are considered to be highly diverse and frequently recombinant pathogens [2,11,12]. Therefore, worldwide surveillance of HBoVs' genetic evolution is necessary.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan

Lin

Chan

Chi

et al. 2020

IJERPH

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Human bocavirus (HBoV) is a causative agent of respiratory and gastrointestinal diseases worldwide. Four HBoV species (HBoV1-4) have been identified so far. Although a previous report has documented the HBoV association with acute gastroenteritis (AGE) in Taiwan, their epidemiology, genetic diversity, and phylogenetic relationships remain unclear. In this study, we focused on an investigation of these unsolved issues, which will help to reveal molecular epidemiology and phylogeny of the circulating HBoV2 in Taiwan. A total of 176 stool samples were collected from children with AGE for this study. PCR amplification and sequencing on the VP1 gene region were used to identify species. Phylogenetic analysis was conducted by maximum-likelihood and neighbor-joining methods. Selection pressure was also estimated to obtain HBoV evolutionary information. Our results showed the prevalence of HBoV in AGE children was 8.5%, of which HBoV1 was the predominant species (6.3%), followed by HBoV2 (2.3%). Phylogenetic analysis showed those Taiwanese HBoV2 strains have significant genetic variability and can be divided into two clusters. One belongs to HBoV2 genotype A and the other forms an independent unclassified cluster. The nucleotide distance between that independent cluster and the known HBoV2 genotypes was more than 5%, suggesting a new HBoV2 genotype. No positive selection site was found and the virus was under purifying selection. This is the first report to reveal HBoV2 genetic diversity and phylogenetic relationships among AGE children in Taiwan. We find that HBoV2 may have been introduced into the country by multiple origins, and a potential new HBoV2 genotype is proposed.

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Complete genome sequence analysis of novel human bocavirus reveals genetic recombination between human bocavirus 2 and human bocavirus 4

Cited by 22 publications

References 27 publications

A study of the human bocavirus replicative genome structures

A study of the human bocavirus replicative genome structures

Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains

Genetic Diversity and Phylogenetic Analysis of Human Bocavirus 2 in Pediatric Patients with Acute Gastroenteritis in Taiwan

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