Complete genome sequence of a novel lytic phage of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight pathogen in rice

My, Pham D.T.; Vinh, Tu Q.; Ngoc, To H.; Anh, Pham N.Q.; Duyen, Le T. M.; Thien, Nguyen M.; Tien, Le T.T.; Phuc, Vo T.; Oanh, Huynh N.; Nga, Le P.; Hoang, Hoang A.

doi:10.1007/s00705-023-05788-5

Cited by 2 publications

(1 citation statement)

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“…In addition to genome properties [10], phage viability in various influence factors, such as temperature, pH, and ultraviolet (UV) radiation, should be investigated for phage production, storage, and practical application purposes. In a previous study of the genome properties with the NCBI accession number OP948730, phage L522 was considered a safe candidate for phage biocontrol [11]. The current study investigated the phage stability under environmental factors and the in vivo efficacy of phage L522 to control BLB disease in rice.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of bacteriophage L522 against bacterial leaf blight of rice in Vietnam

My,

Tien,

Nga

et al. 2024

J App Biol Biotech

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Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a destructive rice disease in tropical Asian countries. The conventional approaches to control BLB, such as cultural practices and using chemical pesticides remain ineffective, whereas bacteriophage biocontrol is considered as a promising strategy. The current study investigated the capacity of phage L522 to control BLB in rice. First, phage L522 showed high tolerance over a wide range of temperatures (4, 20, 30, 37, and 50 o C) and pH (4-11) and was also less affected by ultraviolet (UV)-A and UV-B light. Second, the inhibition time of the phage on the growth of Xoo in tryptone soya broth was about 45 h in the in vitro test. More interestingly, in the in vivo trial on rice plants, the efficacy of phage treatment at a concentration of approximately 10 9 PFU/mL was equivalent to that of a popular commercial pesticide. This study indicated that phage L522 should be a suitable and environmental-friendly alternative to control BLB, especially applicable to soil and climatic conditions in Vietnam. ARTICLE HIGHLIGHTS• Phage L522 showed a high stability under various temperatures, pH, and ultraviolet light. • In the in vitro test, phage L522 controlled the growth of Xanthomonas oryzae pv. oryzae (Xoo) for approximately 45 h. • In the in vivo trial, phage L522 had an equivalent efficacy against bacterial leaf blight in rice compared to a commercial pesticide.

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Section: Introductionmentioning

confidence: 99%