Complete Genome Sequence of a Variant Pseudorabies Virus Strain Isolated in Central China

Xiang, Shuangshuang; Zhou, Zhi; Hu, Xule; Li, Yingying; Zhang, Chaolin; Wang, Juan; Li, Xiangdong; Tan, Feifei; Tian, Kegong

doi:10.1128/genomea.00149-16

Cited by 11 publications

(7 citation statements)

References 6 publications

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“…PRV-GFP, PRRSV BJ-4, VSV-GFP, H1N1-PR8, HSV1-F, and EdU-labeled PRV HN1201 were used as previously described (Wang et al, 2020a). PRV HN1201 was a gift from Ke-Gong Tian (Henan Agricultural University, China) (Xiang et al, 2016). MHV A59 was a gift from Shao-Bo Xiao (Huazhong Agricultural University, China) (Ding et al, 2017).…”

Section: Cells and Virusesmentioning

confidence: 99%

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry

et al. 2021

Sci. China Life Sci.

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Viruses utilize cellular lipids and manipulate host lipid metabolism to ensure their replication and spread. Therefore, the identification of lipids and metabolic pathways that are suitable targets for antiviral development is crucial. Using a library of compounds targeting host lipid metabolic factors and testing them for their ability to block pseudorabies virus (PRV) and vesicular stomatitis virus (VSV) infection, we found that U18666A, a specific inhibitor of Niemann-Pick C1 (NPC1), is highly potent in suppressing the entry of diverse viruses including pseudotyped severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). NPC1 deficiency markedly attenuates viral growth by decreasing cholesterol abundance in the plasma membrane, thereby inhibiting the dynamics of clathrin-coated pits (CCPs), which are indispensable for clathrin-mediated endocytosis. Significantly, exogenous cholesterol can complement the dynamics of CCPs, leading to efficient viral entry and infectivity. Administration of U18666A improves the survival and pathology of PRV-and influenza A virus-infected mice. Thus, our studies demonstrate a unique mechanism by which NPC1 inhibition achieves broad antiviral activity, indicating a potential new therapeutic strategy against SARS-CoV-2, as well as other emerging viruses.

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Section: Cells and Virusesmentioning

confidence: 99%

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry

et al. 2021

Sci. China Life Sci.

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“…PRV-GFP and PRV-QXX were used as previously described [31,32]. PRV HN1201 [33] was a gift from Professor Ke-Gong Tian (Henan Agricultural University, China). Vesicular stomatitis virus (VSV)-GFP, Newcastle disease virus (NDV)-GFP, influenza virus (H1N1 PR8), and Sendai virus (SeV) [34] were gifts from Yong-Tao Li (Henan Agricultural University, China).…”

Section: Cells and Virusesmentioning

confidence: 99%

An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals

et al. 2020

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“…Because of the wide application of live-attenuated vaccines, PRV has been well controlled in most countries, including China [ 11 , 12 , 13 ]. Nevertheless, novel PRV variants with enhanced virulence broke out in pig farms in China in 2011 [ 14 , 15 , 16 , 17 , 18 , 19 ]. Animal experiments demonstrated that classic live vaccines failed to provide complete protection against novel PRV variants [ 10 , 12 , 14 , 17 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Comparative genome analysis revealed that virulent variant PRV acquired considerable mutations [ 14 , 15 , 16 , 18 , 22 , 23 , 24 , 25 , 26 ]. One study demonstrated that the genomic changes in gB contributed to the enhanced viral virulence of novel PRV variants [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Sequence Analysis of Pseudorabies Virus Clinical Isolates from Pigs in China between 2012 and 2017 in China

Wang

Liu

et al. 2021

Viruses

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Pseudorabies virus (PRV) is an economically significant swine infectious agent. A PRV outbreak took place in China in 2011 with novel virulent variants. Although the association of viral genomic variability with pathogenicity is not fully confirmed, the knowledge concerning PRV genomic diversity and evolution is still limited. Here, we sequenced 54 genomes of novel PRV variants isolated in China from 2012 to 2017. Phylogenetic analysis revealed that China strains and US/Europe strains were classified into two separate genotypes. PRV strains isolated from 2012 to 2017 in China are highly related to each other and genetically close to classic China strains such as Ea, Fa, and SC. RDP analysis revealed 23 recombination events within novel PRV variants, indicating that recombination contributes significantly to the viral evolution. The selection pressure analysis indicated that most ORFs were under evolutionary constraint, and 19 amino acid residue sites in 15 ORFs were identified under positive selection. Additionally, 37 unique mutations were identified in 19 ORFs, which distinguish the novel variants from classic strains. Overall, our study suggested that novel PRV variants might evolve from classical PRV strains through point mutation and recombination mechanisms.

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Complete Genome Sequence of a Variant Pseudorabies Virus Strain Isolated in Central China

Abstract: Pseudorabies virus (PRV) variants have been prevalent in China since 2011 and have caused huge economic losses to the Chinese pig industry. Here, we report the genome sequence of a PRV variant HN1201 that was isolated from diseased animals in central China in 2011.

Cited by 11 publications

References 6 publications

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry

An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals

Whole-Genome Sequence Analysis of Pseudorabies Virus Clinical Isolates from Pigs in China between 2012 and 2017 in China

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