Complete genome sequence of a novel alternavirus isolated from the phytopathogenic fungus Colletotrichum fioriniae

Xu, Xiaowen; Li, Jincang; Hai, Du; Wang, Yixun; Li, Jinying; Zha, Yuping

doi:10.1007/s00705-024-06010-w

Arch Virol

2024

DOI: 10.1007/s00705-024-06010-w

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Complete genome sequence of a novel alternavirus isolated from the phytopathogenic fungus Colletotrichum fioriniae

Xiaowen Xu,

Jincang Li,

Du Hai

et al.

Abstract: A novel double-strand RNA(dsRNA) mycovirus, named "Colletotrichum oriniae alternavirus1(CfAV1)", was isolated from the strain CX7 of Colletotrichum oriniae, the causal agent of Walnut anthracnose. The complete genome of CfAV1 is composed of three dsRNA segments, dsRNA1(3528nt), dsRNA2(2485nt), and dsRNA3(2481nt). RNA-dependent RNA polymerase (RdRp) is encoded by dsRNA1, and dsRNA2 and dsRNA3, respectively, encode two hypothetical proteins. Based on multiple sequence alignments and phylogenetic analysis, CfAV1 … Show more

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Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum

Dou,

Li,

Hao

et al. 2024

Front. Plant Sci.

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IntroductionGrape is of high economic value. Colletotrichum viniferum, a pathogen causing grape ripe rot and leaf spot, threatens grape production and quality.MethodsThis study investigates the interplay between C. viniferum by Cytological study and transcriptome sequencing.ResultsDifferent grapevine germplasms, V. vinifera cv. Thompson Seedless (TS), V. labrusca accession Beaumont (B) and V. piasezkii Liuba-8 (LB-8) were classified as highly sensitive, moderate resistant and resistant to C. viniferum, respectively. Cytological study analysis reveals distinct differences between susceptible and resistant grapes post-inoculation, including faster pathogen development, longer germination tubes, normal appressoria of C. viniferum and absence of white secretions in the susceptible host grapevine. To understand the pathogenic mechanisms of C. viniferum, transcriptome sequencing was performed on the susceptible grapevine “TS” identifying 236 differentially expressed C. viniferum genes. These included 56 effectors, 36 carbohydrate genes, 5 P450 genes, and 10 genes involved in secondary metabolism. Fungal effectors are known as pivotal pathogenic factors that modulate plant immunity and affect disease development. Agrobacterium-mediated transient transformation in Nicotiana benthamiana screened 10 effectors (CvA13877, CvA01508, CvA05621, CvA00229, CvA07043, CvA05569, CvA12648, CvA02698, CvA14071 and CvA10999) that inhibited INF1 (infestans 1, P. infestans PAMP elicitor) induced cell death and 2 effectors (CvA02641 and CvA11478) that induced cell death. Additionally, transcriptome analysis of “TS” in response to C. viniferum identified differentially expressed grape genes related to plant hormone signaling (TGA, PR1, ETR, and ERF1/2), resveratrol biosynthesis genes (STS), phenylpropanoid biosynthesis genes (PAL and COMT), photosynthetic antenna proteins (Lhca and Lhcb), transcription factors (WRKY, NAC, MYB, ERF, GATA, bHLH and SBP), ROS (reactive oxygen species) clearance genes (CAT, GSH, POD and SOD), and disease-related genes (LRR, RPS2 and GST).DiscussionThis study highlights the potential functional diversity of C. viniferum effectors. Our findings lay a foundation for further research of infection mechanisms in Colletotrichum and identification of disease response targets in grape.

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Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum

Dou,

Li,

Hao

et al. 2024

Front. Plant Sci.

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Complete genome sequence of a novel alternavirus isolated from the phytopathogenic fungus Colletotrichum fioriniae

Cited by 1 publication

References 29 publications

Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum

Histological and transcriptomic insights into the interaction between grapevine and Colletotrichum viniferum

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