Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken

Sun, Tao; Wang, Ximei; Han, Wei; Ma, Xiaowei; Yin, Weidong; Fang, Baohai; Lin, Xiao; Li, Yang

doi:10.1007/s00705-020-04764-7

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Several prior epidemiological findings have revealed the global occurrence of ALV infection [ 19 , 20 , 21 ]. As a retrovirus, ALV possesses the capacity to generate genetic diversity and undergo rapid evolution due to its error-prone RNA polymerase and frequent recombination events [ 22 , 23 , 24 ], which makes the prevention and control of the virus much more challenging. The most effective method currently to manage ALV infection lies in the implementation of population eradication.…”

Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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Avian leukosis virus (ALV) is an avian oncogenic retrovirus that can impair immunological function, stunt growth and decrease egg production in avian flocks. The capsid protein (P27) is an attractive candidate for ALV diagnostics. In the present study, a new hybridoma cell (1F8) stably secreting an anti-P27 monoclonal antibody (mAb) was developed. The mAb exhibited a high affinity constant (Ka) of 8.65 × 106.0 L/mol, and it could be used for the detection of ALV-A/B/J/K strains. Moreover, a total of eight truncated recombinant proteins and five synthetic polypeptides were utilized for the identification of the B-cell epitopes present on P27. The results revealed that 218IIKYVLDRQK227 was the minimal epitope recognized by 1F8, which had never been reported before. Additionally, the epitopes could strongly react with different ALV subgroup’s specific positive serum and had a complete homology among all the ALV subgroups strains. Finally, a new sandwich ELISA method was created for the detection of ALV antigens, demonstrating increased sensitivity compared to a commercially available ELISA kit. These results offer essential knowledge for further characterizing the antigenic composition of ALV P27 and will facilitate the development of diagnostic reagents for ALV.

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Section: Discussionmentioning

confidence: 99%

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Wang,

Liu,

Dou

et al. 2024

CIMB

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“…In previous studies, an exogenous ALV strain TW-3593 isolated from Taiwan, China, in 2008, and several strains of fowl glioma virus (FGV) isolated from Japan were reported to be from local chickens, which showed high amino acid sequence similarity (more than 90%) to the gp85 gene of Chinese ALV-K isolates, but low similarity to other ALVs ( Chang et al, 2013 ; Guo et al, 2023 ). Subsequently, ALV-K isolates were also found in yellow chickens from Guangdong and Shandong, China ( Li et al, 2016 , 2019 ; Sun et al, 2020 ). Recently, our research group found that ALV-K is widespread in endemic chickens in Jiangxi Province, southern China, and the positivity rate in ALV-positive samples has been increasing yearly.…”

Section: Introductionmentioning

confidence: 99%

Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023

Zhang,

Li,

Lin

et al. 2024

Front. Microbiol.

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Avian leukosis virus subgroup K (ALV-K) is a new subgroup of avian leukosis virus (ALV) that was first identified in Chinese native chickens in recent years. To further understand the molecular epidemiology and evolutionary diversity of ALV-K, this study investigated the molecular epidemiology of 73,664 chicken plasma samples collected from Jiangxi native chicken flocks. The results showed that ALV-J was the most predominant ALV subtype in Jiangxi native chickens, with a high positivity rate of 4.34%. From 2021 to 2023, there was a gradual upward trend in the proportion of positive numbers of ALV-K among ALV-positive samples, and there was a trend of outbreaks. ALV-J and ALV-K were the main co-infection patterns. Genetic evolutionary analysis based on ALV-K gp85 gene showed that the isolated ALV-K in this study were distributed in various branches of the evolutionary tree with genetic diversity. The homology results showed that the amino acid homology of the isolated ALV-K gp85 gene ranged from 33.9 to 88.1% with the reference strains of subtypes A, B, C, D, E, and J, and from 91.9 to 100% with the other ALV-K reference strains. Multiple mutations were present in the ALV-K gp85, and especially significant mutations were found in the highly variable region hr2. The results of ALV-K replication efficiency showed that the replication efficiency of ALV-K was significantly lower than that of ALV-J. These results enriched the genome sequence data of ALV-K in Chinese geoducks, and laid the foundation for further research on the pathogenesis and prevention of ALV-K.

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“…The gp85 gene encodes the most variable structural protein in the ALV genome, which is associated with virus neutralization and viral host range [ 15 , 16 ]. Based on this feature, ALV can mutate or recombine into new retrovirus strains [ 17 – 19 ]. In order to prevent and control the disease, scientists have developed vaccines, but the effect of vaccines cannot provide sufficient protection for chickens [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Advances on genetic and genomic studies of ALV resistance

Wei

et al. 2022

J Animal Sci Biotechnol

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Avian leukosis (AL) is a general term for a variety of neoplastic diseases in avian caused by avian leukosis virus (ALV). No vaccine or drug is currently available for the disease. Therefore, the disease can result in severe economic losses in poultry flocks. Increasing the resistance of poultry to ALV may be one effective strategy. In this review, we provide an overview of the roles of genes associated with ALV infection in the poultry genome, including endogenous retroviruses, virus receptors, interferon-stimulated genes, and other immune-related genes. Furthermore, some methods and techniques that can improve ALV resistance in poultry are discussed. The objectives are willing to provide some valuable references for disease resistance breeding in poultry.

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Complete genome sequence of a novel recombinant avian leukosis virus isolated from a three-yellow chicken

Cited by 5 publications

References 11 publications

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application

Detection and genetic diversity of subgroup K avian leukosis virus in local chicken breeds in Jiangxi from 2021 to 2023

Advances on genetic and genomic studies of ALV resistance

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