Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses

Absalón, Ángel E.; Morales-Garzón, Andrés; Vera-Hernández, Pedro Fernando; Cortés-Espinosa, Diana V.; Uribe-Ochoa, Sara M.; García, Laura J.; Lucio-Decanini, Eduardo

doi:10.1016/j.virol.2016.11.006

Cited by 27 publications

(18 citation statements)

References 22 publications

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“…Viral particles of FAdV-I were found in the liver, intestines, and pancreas. The organs where this virus can be detected are the liver, intestines, caecal tonsils, proventriculus, bursa of Fabricius, thymus, spleen, lungs and kidneys [ 32 , 33 , 34 , 35 ], especially in the first week of infection. Based on the pancreatic tissue samples that were positive for FAdV-I (5/28) ( Table 7 ), we confirmed the importance of this organ for diagnosis of diseases caused by fowl adenovirus and even in cases of pancreatitis [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks

Torre

Astolfi-Ferreira

Ferreira

2018

Veterinary Sciences

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Enteric viruses play an important role in the Brazilian poultry industry due to the economic impact of resulting low yields of broilers, layers, and breeders. The most common enteric viruses affecting commercial flocks in Brazil include Fowl Adenovirus of group I (FAdV-I), Chicken Parvovirus (ChPV), Chicken Astrovirus (CAstV), Avian Nephritis Virus (ANV), Infectious Bronchitis Virus (IBV), Avian Reovirus (AReo), and Avian Rotavirus (ARtV). The aim of this study was to identify single and multiple infections using data obtained from 270 samples from eleven Brazilian states, corresponding to the period between 2010 and 2017. This was accompanied by an analysis of the relationship between the age of birds, clinical signs, and geographical distribution, using Polymerase Chain Reaction (PCR) and Reverse Transcription-PCR (RT-PCR) techniques. Twenty-five profiles of virus combinations were detected. Single infections were encountered in 86.3% of samples, and multiple infections were present in the remaining 13.7%. Both single and multiple infections affected all kinds of commercial chickens with digestive problems, stunting syndrome, decreases in egg and meat production, increased mortality, and respiratory signs. FAdV-I, ChPV, CAstV, ANV, and ARtV were mostly detected in young broilers, in contrast with IBV, which was detected in hens from one to greater than 51 weeks of age. These results exhibit the complexity of enteric diseases and the still poorly understood role of each pathogen as a unique etiological agent.

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Section: Discussionmentioning

confidence: 99%

Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks

Torre

Astolfi-Ferreira

Ferreira

2018

Veterinary Sciences

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“…FAdV strains sequenced in this study were either isolated from documented clinical cases, or purchased from the American Type Culture Collection (Manassas, VA), as well as kindly supplied by H. M. Hafez (Institute of Poultry Diseases, Berlin, Germany) and Vaxxinova Japan K.K. (Tokyo, Japan) ( Supplementary Table S1) [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29]. Besides FAdV-D and FAdV-E strains, we sequenced further strains of species FAdV-A (including the hitherto unavailable OTE reference genome) and FAdV-C (including, for the first time, the complete FAdV-10 genome), in order to increase robustness of the analysis.…”

Section: Virus Strains and Dna Preparationmentioning

confidence: 99%

Fowl Adenovirus (FAdV) Recombination with Intertypic Crossovers in Genomes of FAdV-D and FAdV-E, Displaying Hybrid Serological Phenotypes

Schachner

González

Endler

et al. 2019

Viruses

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After analyzing 27 new genomes from fowl adenovirus (FAdV) field isolates and so-far unsequenced prototypes, we report the first evidence for recombination in FAdVs. Recombination was confined to species FAdV-D and FAdV-E, accommodating the largest number of, and the intraspecies-wise most differentiated, types. The majority of detected events occurred in FAdV-E, involving segments with parental origin of all constitutive types. Together with the diversity of breakpoints, this suggests widespread recombination in this species. With possible constraints through species-specific genes and diversification patterns, the recombinogenic potential of FAdVs attains particular interest for inclusion body hepatitis (IBH), an important disease in chickens, caused by types from the recombination-prone species. Autonomously evolving, recombinant segments were associated with major sites under positive selection, among them the capsid protein hexon and fiber genes, the right-terminal ORFs 19, 25, and the ORF20/20A family. The observed mosaicism in genes indicated as targets of adaptive pressures points toward an immune evasion strategy. Intertypic hexon/fiber-recombinants demonstrated hybrid neutralization profiles, retrospectively explaining reported controversies on reference strains B3-A, T8-A, and X11-A. Furthermore, cross-neutralization supported sequence-based evidence for interdomain recombination in fiber and contributed to a tentatively new type. Overall, our findings challenge the purported uniformity of types responsible for IBH, urging more complete identification strategies for FAdVs. Finally, important consequences arise for in vivo studies investigating cross-protection against IBH.

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“…El FAdV-1 es responsable por causar dos principales enfermedades, la Hepatitis por Cuerpos de Inclusión (IBH) y el Síndrome del Hidropericardio (HPS) (Zhao et al, 2015). Hay algunos casos en donde el virus ha sido aislado de aves sin signos de enfermedad, por lo que se requiere un mayor desarrollo de técnicas que permitan diferenciar cepas patogénicas y no patogénicas dentro del mismo serotipo del virus (Absalón et al, 2017). En el Ecuador ya se ha reportado la presencia del serotipo 4 del FAdV-I como agente causal del Síndrome del Hidropericardio (Mazaheri et al, 1998), pero también es importante recalcar que todos los serotipos están relacionados con la IBH (Adair y Fitzgerald, 2008), y de esta forma se debe mantener un control epidemiológico de todas las cepas y serotipos que circulan por LA GRANJA:Revista de Ciencias de la Vida 28 (2) Byron Humberto Puga Torres y Antonio José Piantino Ferreira la región.…”

Section: Análisis Filogenéticounclassified

Caracterización molecular del adenovirus aviar en pollos comerciales del Ecuador

Duque¹,

Quezada²,

Torres³

et al. 2018

lgr

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El Adenovirus Aviar del grupo I (FAdV-I), es considerado como un patógeno de mucha importancia en la industria avícola en el Ecuador y a nivel mundial. La Hepatitis por Cuerpos de Inclusión (IBH) y el Síndrome del Hidropericardio (HPS), son las principales enfermedades asociadas a este virus, y a pesar de ser un agente etiológico que por lo general se encuentra relacionado a enfermedades inmunodepresoras como Anemia Infecciosa de las Aves o la enfermedad de Gumboro, recientes estudios han demostrado la actividad patológica del FAdV-I como responsable primario de estas enfermedades. Desde la década de 1990, se reportaron varios brotes importantes de IBH y HPS en muchos países de América Latina incluyendo el Ecuador, logrando identificar principalmente al serotipo 4 del FAdV-I como agente causal de estos brotes. El objetivo de este estudio fue determinar por métodos moleculares como la Reacción en Cadena de la Polimerasa (PCR), la presencia de los serotipos del FAdV-I que se encuentran circulando en granjas de aves comerciales en Ecuador. Fueron colectados diferentes órganos de aves con problemas entéricos en la zona norte del Ecuador, y posteriormente impregnados en tarjetas FTA para su transporte y procesamiento. 4/13 (30,8%) muestras fueron positivas a FAdV-I y mediante el secuenciamiento y análisis bioinformático del ADN amplificado por la PCR, se caracterizaron los serotipos 6 y 11 del FAdV-I. Estos datos nos demuestran la variedad de serotipos presentes en granjas avícolas del Ecuador y que podrían estar influenciando en el estado sanitario de la industria avícola del país.

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Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses

Cited by 27 publications

References 22 publications

Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks

Enteric Virus Diversity Examined by Molecular Methods in Brazilian Poultry Flocks

Fowl Adenovirus (FAdV) Recombination with Intertypic Crossovers in Genomes of FAdV-D and FAdV-E, Displaying Hybrid Serological Phenotypes

Caracterización molecular del adenovirus aviar en pollos comerciales del Ecuador

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