Complete Genome Sequence of a
            <i>Tomato Brown Rugose Fruit Virus</i>
            Isolated in the United States

Chanda, Bidisha; Rivera, Yazmín; Nunziata, Schyler O.; Galvez, Marco; Gilliard, Andrea; Ling, Kai-Shu

doi:10.1128/mra.00630-20

Cited by 23 publications

(16 citation statements)

References 17 publications

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“…MT002973) was collected from tomato in Southern California in August 2018 [18] and a pure ToBRFV isolate (GenBank accession no. MT002973) was generated through local lesion host and maintained on 'Moneymaker' tomato plants [19]. Active cultures of both tobamoviruses were maintained in their respective plants inside an insect-proof bug dome (BioQuip Products, USA) through mechanical inoculation in a containment greenhouse with temperature maintained at 25 to 30 °C at the U.S.…”

Section: Sources Of Cgmmv and Tobrfvmentioning

confidence: 99%

“…ToBRFV is considered more virulent than other known tomato-infecting tobamoviruses, as it breaks the popular Tm-2 2 resistance gene which is present in many commercial tomato cultivars [6,7]. ToBRFV outbreaks in greenhouse tomatoes have been reported in countries around the world, including China [8] and Palestine [9] in Asia, Egypt in Africa [10], Germany [11], Greece [12], Italy [13]), Turkey [14] and the United Kingdom [15] in Europe, and Mexico [16,17], the United States [18,19] and Canada [20] in North America. In response to the global outbreaks of ToBRFV, the United States Department of Agriculture issued a Federal Order in November 2019 [21] and the Europe Union has declared a quarantine status for ToBRFV [22].…”

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confidence: 99%

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Effectiveness of disinfectants against the spread of tobamoviruses: Tomato brown rugose fruit virus and Cucumber green mottle mosaic virus

Chanda

Shamimuzzaman

Gilliard

et al. 2021

Virol J

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Background Tobamoviruses, including tomato brown rugose fruit virus (ToBRFV) on tomato and pepper, and cucumber green mottle mosaic virus (CGMMV) on cucumber and watermelon, have caused many disease outbreaks around the world in recent years. With seed-borne, mechanical transmission and resistant breaking traits, tobamoviruses pose serious threat to vegetable production worldwide. With the absence of a commercial resistant cultivar, growers are encouraged to take preventative measures to manage those highly contagious viral diseases. However, there is no information available on which disinfectants are effective to deactivate the virus infectivity on contaminated hands, tools and equipment for these emerging tobamoviruses. The purpose of this study was to evaluate a collection of 16 chemical disinfectants for their effectiveness against mechanical transmission of two emerging tobamoviruses, ToBRFV and CGMMV. Methods Bioassay was used to evaluate the efficacy of each disinfectant based on virus infectivity remaining in a prepared virus inoculum after three short exposure times (10 s, 30 s and 60 s) to the disinfectant and inoculated mechanically on three respective test plants (ToBRFV on tomato and CGMMV on watermelon). Percent infection of plants was measured through symptom observation on the test plants and the presence of the virus was confirmed through an enzyme-linked immunosorbent assay with appropriate antibodies. Statistical analysis was performed using one-way ANOVA based on data collected from three independent experiments. Results Through comparative analysis of percent infection of test plants, a similar trend of efficacy among 16 disinfectants was observed between the two pathosystems. Four common disinfectants with broad spectrum activities against two different tobamoviruses were identified. Those effective disinfectants with 90–100% efficacy against both tobamoviruses were 0.5% Lactoferrin, 2% Virocid, and 10% Clorox, plus 2% Virkon against CGMMV and 3% Virkon against ToBRFV. In addition, SP2700 generated a significant effect against CGMMV, but poorly against ToBRFV. Conclusion Identification of common disinfectants against ToBRFV and CGMMV, two emerging tobamoviruses in two different pathosystems suggest their potential broader effects against other tobamoviruses or even other viruses.

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Section: Sources Of Cgmmv and Tobrfvmentioning

confidence: 99%

mentioning

confidence: 99%

Effectiveness of disinfectants against the spread of tobamoviruses: Tomato brown rugose fruit virus and Cucumber green mottle mosaic virus

Chanda

Shamimuzzaman

Gilliard

et al. 2021

Virol J

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“…ToBRFV is a (+) single-stranded RNA virus and belongs to the genus Tobamovirus in the family Virgaviridae. The virus particles have a rigid rod shape, and its genome has a length of 6.2 to 6.4 kb, encoding four proteins: two replication-related proteins of 126 and 183 kDa resulting from the partial suppression of a stop codon; a 30 kDa movement protein (MP); and a 17.5 kDa coat protein (CP) [32][33][34].…”

Section: Tomato Brown Rugose Fruit Virusmentioning

confidence: 99%

“…ToBRFV is an emerging virus with the capacity to infect tomato cultivars harboring R genes Tm-1, Tm-2, or Tm-2 2 , which confer resistance to tobamoviruses [34]. Hence, the relevance of ToBRFV relies on the severe losses it might cause to tomato crops as a result of symptoms on fruit.…”

Section: Tomato Brown Rugose Fruit Virusmentioning

confidence: 99%

Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

García‐Estrada

Diaz‐Lara

Aguilar-Molina

et al. 2022

Viruses

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Tomato is the most economically important vegetable crop worldwide and the second most important for Mexico. However, viral diseases are among the main limiting factors that affect the productivity of this crop, causing total losses in some cases. This review provides key information and findings on the symptoms, distribution, transmission, detection, and management of diseases caused by viruses of major importance in tomato crops in Mexico. Currently, about 25 viruses belonging to nine different families have been reported infecting tomato in Mexico, but not all of them cause economically significant diseases. Viruses of economic importance include tomato brown rugose fruit virus (ToBRFV), tomato spotted wilt virus (TSWV), tomato yellow leaf curl virus (TYLCV), pepino mosaic virus (PepMV), and tomato marchitez virus (ToMarV). The topics discussed here will provide updated information about the status of these plant viruses in Mexico as well as diverse management strategies that can be implemented according to the specific circumstances of each viral pathosystem. Additionally, a list of tomato-affecting viruses not present in Mexico that are continuous threats to the crop health is included.

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“…In the past four years, there have been only nine ONT plant virus publications recorded in the National Center for Biotechnology Information Pubmed database on approaches ranging from genotyping viruses in potato [ 6 ] and yam [ 7 ] to plant virus surveillance [ 8 ] and whole-genome sequencing [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Application of Oxford Nanopore Technology to Plant Virus Detection

Liefting

Waite

Thompson

2021

Viruses

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The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification. The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. Here, we provide a detailed step-by-step protocol using the ONT Flongle platform that we have developed for the routine application on a range of symptomatic post-entry quarantine and domestic surveillance plant samples. The aim of this methods paper is to highlight ONT’s feasibility as a valuable component to the diagnostician’s toolkit and to hopefully stimulate other laboratories towards the eventual goal of integrating high-throughput sequencing technologies as validated plant virus diagnostic methods in their own right.

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Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States

Cited by 23 publications

References 17 publications

Effectiveness of disinfectants against the spread of tobamoviruses: Tomato brown rugose fruit virus and Cucumber green mottle mosaic virus

Effectiveness of disinfectants against the spread of tobamoviruses: Tomato brown rugose fruit virus and Cucumber green mottle mosaic virus

Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

Application of Oxford Nanopore Technology to Plant Virus Detection

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