Complete Genome Sequence of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain from Two Genotype 1 Modified Live Virus Vaccine Strains

Renson, Patricia; Touzain, Fabrice; Lebret, Arnaud; Dimna, Mireille Le; Quenault, Hélène; Normand, Valérie; Claude, Jean‐Baptiste; Pez, Floriane; Rose, Nicolas; Blanchard, Yannick; Bourry, Olivier

doi:10.1128/genomea.00454-17

Cited by 18 publications

(23 citation statements)

References 9 publications

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“…An experimental study from France revealed that the Porcilis/ Unistrain recombinant virus describe above (Renson et al, 2017) seems to be more virulent than the two parent PRRSV-1 vaccine viruses (Eclercy et al, 2019). However, this study did not assess if the increased virulence of the recombinant virus reflected that the recombinant virus was adapted to swine or if it was a result of the recombination per se.…”

Section: F I G U R Ementioning

confidence: 91%

“…In two of these reports, the Unistrain vaccine strain Amervac was part of the recombined virus, but the recombination break point differed from the 'Horsens' virus. Recombination between two PRRSV-1 vaccine strains has only been reported once from France where a recombinant between the 'DV' vaccine strain (Porcilis PRRS, MSD) and the 'Amervac' strain (Unistrain, Hipra) was detected in a single herd (Renson et al, 2017).…”

Section: F I G U R Ementioning

confidence: 99%

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A recombination between two Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV‐1) vaccine strains has caused severe outbreaks in Danish pigs

Kvisgaard

Kristensen

Ryt-Hansen

et al. 2020

Transbound Emerg Dis

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Porcine reproductive and respiratory syndrome virus (PRRSV) is prevalent in Danish swine herds. In July 2019, PRRSV‐1 was detected in a PRRSV‐negative boar station and subsequently spread to more than 38 herds that had received semen from the boar station. Full genome sequencing revealed a sequence of 15.098 nucleotides. Phylogenetic analyses showed that the strain was a recombination between the Amervac strain (Unistrain PRRS vaccine; Hipra) and the 96V198 strain (Suvaxyn PRRS; Zoetis AH). The major parent was the 96V198 strain that spanned ORFs 1–2 and part of ORF 3 and the minor parent was the Amervac strain, which constituted the remaining part of the genome. The virus seems to be highly transmissible and has caused severe disease in infected herds despite a high level of genetic identity to the attenuated parent strains. The source of infection was presumable a neighbouring farm situated 5.8 km from the boar station.

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Section: F I G U R Ementioning

confidence: 91%

Section: F I G U R Ementioning

confidence: 99%

A recombination between two Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV‐1) vaccine strains has caused severe outbreaks in Danish pigs

Kvisgaard

Kristensen

Ryt-Hansen

et al. 2020

Transbound Emerg Dis

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“…Therefore, the simultaneous or contemporaneous use of different PRRSV MLV vaccines poses a risk of recombination. Renson et al reported a recombinant PRRSV-1 strain derived from two MLV vaccines (Unistrain PRRS and Porcilis PRRS) in a pig farm in France [ 9 ]. In a later study from the same group, animals infected with the recombinant strain did not show significant clinical signs, although the recombinant strain demonstrated increased excretion and transmission capabilities compared to parental vaccine strains [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

Genís¹,

Kvisgaard

Larsen

et al. 2020

Pathogens

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This study assessed the impact of a PRRSV (porcine reproductive and respiratory syndrome virus) recombinant strain (Horsens strain) on the reproductive performance of naïve pregnant sows in the last third of gestation. Fifteen sows were included: four negative reproductive controls (NTX), five infected with a PRRSV-1 field strain (Olot/91, T01), and six infected with the recombinant PRRSV-1 strain (Horsens strain, T02). Piglets were monitored until weaning. Reproductive performance was the primary variable. In sows, viremia and nasal shedding (T01 and T02 groups), and, in piglets, viral load in blood and in lungs, as well as macroscopic lung lesions (T01 and T02 groups), were the secondary variables. The reproductive performance results were numerically different between the two challenged groups. Moreover, viral loads in blood were 1.83 × 106 ± 9.05 × 106 copies/mL at farrowing, 1.05 × 107 ± 2.21 × 107 copies/mL at weaning from piglets born from T01 animals and 1.64 × 103 ± 7.62 × 103 copies/mL at farrowing, 1.95 × 103 ± 1.17 × 104 copies/mL at weaning from piglets born from T02 sows. Overall, 68.8% of T01 piglets and 38.1% of T02 piglets presented mild lung lesions. In conclusion, the results suggest that Horsens strain is less virulent than the field strain Olot/91 under these experimental conditions.

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“…In Central and Western Europe, only Lelystad virus-like circulates, but in Eastern Europe, all subtypes are present [6]. The PRRS virus genome is not segmented, having a positive polarity and size of about 15 kb [7]. The genome contains 8 open reading frames (ORF) for encoding structural and non-structural proteins of the virus.…”

Section: Introductionmentioning

confidence: 99%

The Performance of Seven Molecular Methods for the Detection of PRRSV

Milićević¹,

Kureljušić²,

Veljović³

et al. 2020

Acta Veterinaria

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Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.

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Complete Genome Sequence of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Strain from Two Genotype 1 Modified Live Virus Vaccine Strains

Cited by 18 publications

References 9 publications

A recombination between two Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV‐1) vaccine strains has caused severe outbreaks in Danish pigs

A recombination between two Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV‐1) vaccine strains has caused severe outbreaks in Danish pigs

Assessment of the Impact of the Recombinant Porcine Reproductive and Respiratory Syndrome Virus Horsens Strain on the Reproductive Performance in Pregnant Sows

The Performance of Seven Molecular Methods for the Detection of PRRSV

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