Complete Genome Sequence of Acinetobacter calcoaceticus CA16, a Bacterium Capable of Degrading Diesel and Lignin

Ho, Margaret T.; Weselowski, Brian; Yuan, Zhongshun

doi:10.1128/genomea.00494-17

Cited by 10 publications

(11 citation statements)

References 19 publications

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“…The features of CA16 complete genomic assembly have been previously deposited to Genbank with the Accession number CP020000 and CP020001 [28]. Briefly, CA16 has a total of 3798 coding genes, 6 rRNA operons and 65 tRNAs.…”

Section: Genomic Sequencing and Phylogenetic Analysismentioning

confidence: 99%

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Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

McDowell

et al. 2020

BMC Biotechnol

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Background: With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective decontamination method is to utilize diesel-degrading microbes as a means for bioremediation. Here, we present a newly isolated and identified strain of Acinetobacter calcoaceticus ('CA16') as a candidate for the bioremediation of diesel-contaminated areas. Results: Acinetobacter calcoaceticus CA16 was able to survive and grow in minimal medium with diesel as the only source of carbon. We determined through metabolomics that A. calcoaceticus CA16 appears to be efficient at diesel degradation. Specifically, CA16 is able to degrade 82 to 92% of aliphatic alkane hydrocarbons (C n H n + 2 ; where n = 12-18) in 28 days. Several diesel-degrading genes (such as alkM and xcpR) that are present in other microbes were also found to be activated in CA16. Conclusions: The results presented here suggest that Acinetobacter strain CA16 has good potential in the bioremediation of diesel-polluted environments.

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Section: Genomic Sequencing and Phylogenetic Analysismentioning

confidence: 99%

“…Previously, canola roots from southwestern Ontario were harvested by our group [28] to isolate their microbes. The sample collection followed standard guidelines required by Environment Canada, and research with the bacterial strains was done in accordance with biosafety procedures at Agriculture and Agri-Food Canada.…”

Section: Isolation Of a Calcoaceticus Ca16mentioning

confidence: 99%

Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

McDowell

et al. 2020

BMC Biotechnol

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“…2015;Islam et al, 2017). A. calcoaceticus and other Acinetobacter members can utilize diesel and lignin as carbon sources (Ho et al, 2017;Ghodake et al, 2009;Ponnambalam et al, 2011). Stenotrophomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Microbial degradation of lignocellulosic biomass: discovery of novel natural lignocellulolytic bacteria

Ahmed¹,

Rahman²,

Hasan³

et al. 2018

bta

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Lignocellulosic biomass is the most abundant biomaterial on Earth and holds enormous potential as a source of fermentable sugars for cost-effective biofuel production. The main obstacle is the intractable and convoluted structure and composition of lignocellulosics which are mainly made of lignin, cellulose and hemicellulose. However, nature has equipped microbes with enzymes that can deconstruct lignocellulose and release fermentable sugars. In this study, we selected bacteria (belonging to 6 different genera) from nature that had been grown in minimal culture media with cellulose or lignin as the sole carbon source, and assessed their lignocellulolytic activities in qualitative and quantitative assays. Through sequence analysis of the 16S rRNA gene, we discovered a novel lignocellulolytic bacterial species, Chryseobacterium gleum, capable of degrading both cellulose and lignin. In addition, a few other bacterial species previously known to degrade either cellulose or lignin were found to have lignocellulolytic activities. The majority of these bacterial species were found to produce extracellular lignocellulolytic enzymes. Lignocellulolytic bacteria identified in this study may serve as sources of genes/enzymes which might find applications in biofuel production and other industrial applications.

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“…Information about the genome sequence of N. panipatense P5:ABC will provide insight into the diversity and mechanisms of hydrocarbon degradation in the environment. During our analysis, we found the presence of several hydrocarbon-degrading and related genes, namely, dioxygenases, rubredoxin, esterase, alkane-1-monooxygenase, alkane sulfonate monooxygenase, formate dehydogenase, 4-carboxymuconolactone decarboxylase, 3-carboxy- cis , cis -muconatecycloisomerase, 3-oxoadipate-enol-lactonase, protocatechuate-3, and salicylaldehyde dehydrogenase ( 14 – 16 ). This knowledge of the gene might be used to improve the bioremediation technologies used in this organism.…”

Section: Genome Announcementmentioning

confidence: 88%

Draft Genome Sequence of Novosphingobium panipatense Strain P5:ABC, Isolated from Hydrocarbon-Contaminated Soil from Noonmati Refinery, Assam, India

Singh

Chettri

Ghosh³

et al. 2017

Genome Announc

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Complete Genome Sequence of Acinetobacter calcoaceticus CA16, a Bacterium Capable of Degrading Diesel and Lignin

Cited by 10 publications

References 19 publications

Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

Microbial degradation of lignocellulosic biomass: discovery of novel natural lignocellulolytic bacteria

Draft Genome Sequence of Novosphingobium panipatense Strain P5:ABC, Isolated from Hydrocarbon-Contaminated Soil from Noonmati Refinery, Assam, India

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