Complete Genome Sequence of Bacillus velezensis YJ11-1-4, a Strain with Broad-Spectrum Antimicrobial Activity, Isolated from Traditional Korean Fermented Soybean Paste

Lee, Hyo‐Jung; Chun, Byung-Hee; Jeon, Hye Hee; Kim, Yeon Bee; Lee, Se Hee

doi:10.1128/genomea.01352-17

Cited by 20 publications

(13 citation statements)

References 8 publications

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“…velezensis is a biocontrol agent that exhibits broad spectrum antimicrobial activity against various foodborne pathogens, and there are no gene families that correlate with human pathogenicity detected in the B . velezensis genome 43 , 44 . Therefore, we presume that the B .…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice

Wang

Pan

et al. 2018

Sci Rep

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Zearalenone (ZEN) is an estrogen-like mycotoxin occurring in food and feeds, and it can cause oxidative damage and apoptosis in the testis, liver, and kidney. A current concern for researchers is how to reduce the harm it causes to humans and animals. In this study, our aim was to isolate and identify a novel and efficient ZEN-detoxifying strain of bacteria, and we aimed to assess the protective effect of the isolated strain on kidney damage caused by ZEN in mice. Our results indicated that a strain of Bacillus velezensis (B. velezensis), named A2, could completely degrade ZEN (7.45 μg/mL) after three days of incubation at 37 °C in the Luria-Bertani (LB) medium. This fermentation broth of the B. velezensis A2 strain was given to mice. The histopathological analysis indicated that the fermentation broth from the B. velezensis A2 strain reduced the degree of renal injury that is induced by ZEN. Furthermore, it greatly reduced the increase in serum levels of creatinine (CRE), uric acid (UA), and urea nitrogen (BUN) caused by ZEN. In addition, B. velezensis A2 strain also significantly inhibited the increase of malonaldehyde (MDA) content, and reversed the decreases of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities caused by ZEN. Studies have shown that ZEN is involved in the regulation of mRNA and protein levels of genes involved in the ER stress-induced apoptotic pathway, such as heavy chain binding protein (BIP), C-/-EBP homologous protein (CHOP), cysteine Aspartate-specific protease-12 (Caspase-12), c-Jun N-terminal kinase (JNK), and BCL2-related X protein (Bcl-2 and Bax). However, when mice were administered the fermentation broth of the B. velezensis A2 strain, it significantly reversed the expressions of these genes in their kidney tissue. In conclusion, our results indicate that the newly identified strain of B. velezensis A2, has a protective effect from renal injury induced by ZEN in mice. This strain has a potential application in the detoxification of ZEN in feed and protects animals from ZEN poisoning.

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Section: Discussionmentioning

confidence: 99%

Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice

Wang

Pan

et al. 2018

Sci Rep

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“…In addition, another study reported that Bacillus velezensis can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi [ 30 , 31 ]. Meanwhile, Bacillus velezensis exhibits broad spectrum antimicrobial activity against various foodborne pathogens [ 32 , 33 ], and there are no gene families that correlate with the human pathogenicity detected in the Bacillus velezensis genome [ 34 ]. In summary, Bacillus velezensis is a probiotic that can be used to decontaminate mycotoxins in production practice.…”

Section: Introductionmentioning

confidence: 99%

The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

Wang

et al. 2018

Toxins

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Zearalenone (ZEN) is an estrogen-like mycotoxin produced by Fusarium that seriously compromises the safety of animal and human health. In this study, our aim was to evaluate the protective effect of Bacillus velezensis A2 against biochemical and pathological changes induced by zearalenone in mice. Kunming mice (n = 40; 25 ± 2 g) were allotted to four treatment groups: a control group (basic feed); a ZEN group (basic feed with a ZEN dose of 60 mg/kg); an A2 strain fermented feed group (150 g of feed mixed with 150 mL of sterile distilled water and inoculated with 5 mL of phosphate buffer salt (PBS) resuspended A2 strain); and an A2 strain fermented ZEN-contaminated feed group. (A2 strain group 150 mL pure bacterial distilled water system mixed with 150 g ZEN-contaminated feed.) Our results showed that the Bacillus velezensis A2 strain can completely degrade the ZEN-contaminated feed within 5 days. (The concentration of ZEN in fermentation was 60 μg/mL.) After the mice fed for 28 days, compared with the control group, the activities of AST and ALT were increased, the activities of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) were decreased, and the amount of creatinine (CRE), blood urea nitrogen (BUN), uric acid (UA), and malondialdehyde (MDA) in the ZEN group were increased in the mice serum (p < 0.05; p < 0.01). However, compared with the ZEN group, these biochemical levels were reversed in the A2 strain fermented feed group and in the A2 strain fermented ZEN-contaminated feed group (p < 0.05; p < 0.01). Furthermore, histopathological analysis only showed pathological changes of the mice liver in the ZEN group. The results showed that Bacillus velezensis A2 as additive could effectively remove ZEN contamination in the feed and protect the mice against the toxic damage of ZEN. In conclusion, Bacillus velezensis A2 has great potential use as a microbial feed additive to detoxify the toxicity of zearalenone in production practice.

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“…Furthermore, these species are frequently detected in fermented foods containing meju and doenjang [24,31,32]. We conclude therefore that B. velezensis may be transferred from environments such as soil to meju, where it becomes a dominant species in during fermentation and then migrates into doenjang [24,31,32].…”

Section: Comparison Of Bacterial Communities Identified Using Culturementioning

confidence: 89%

“…B. velezensis is widely detected in sources such as soil, wheat anthers, and rivers [26][27][28]; and it is used as a biocontrol agent in agriculture [28][29][30]. Furthermore, these species are frequently detected in fermented foods containing meju and doenjang [24,31,32]. We conclude therefore that B. velezensis may be transferred from environments such as soil to meju, where it becomes a dominant species in during fermentation and then migrates into doenjang [24,31,32].…”

Section: Comparison Of Bacterial Communities Identified Using Culturementioning

confidence: 92%

Culture-dependent and -independent investigations of bacterial migration into doenjang from its components meju and solar salt

et al. 2020

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We determined bacterial migration into doenjang from its components, meju and solar salt using culture-based and 16S rRNA gene-based culture-independent techniques (pyrosequencing of total DNA). Pyrosequencing results suggested that the bacterial communities of meju, but not solar salt, significantly affected those of doenjang communities. Culture-based pyrosequencing analysis yielded similar results. These results indicate that most predominant bacterial species in doenjang migrated from meju, not solar salt. We therefore believe that the present study is one of the most comprehensive comparisons of bacterial communities of fermented soybeans using culture-dependent and-independent methods. Furthermore, pyrosequencing of the V3 and V4 regions of bacterial 16S rRNA did not distinguish among Bacillus amyloliquefaciens, B. siamensis, and B. velezensis as well as between Enterococcus faecium and E. hirae.

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Complete Genome Sequence of Bacillus velezensis YJ11-1-4, a Strain with Broad-Spectrum Antimicrobial Activity, Isolated from Traditional Korean Fermented Soybean Paste

Cited by 20 publications

References 8 publications

Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice

Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice

The Protective Role of Bacillus velezensis A2 on the Biochemical and Hepatic Toxicity of Zearalenone in Mice

Culture-dependent and -independent investigations of bacterial migration into doenjang from its components meju and solar salt

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