Complete genome sequence of broad bean true mosaic virus

Petrzik, Karel

doi:10.1007/s00705-010-0668-2

Cited by 4 publications

(1 citation statement)

References 4 publications

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“…Moreover, gerbera plants were tested on TSWV and chrysanthemum plants on Chrysanthemum virus B (CVB, Carlavirus genus, Betaflexiviridae family) and TSWV. All the primers used in the experiment are summarised in Table 2 [ 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 ]. The resulting PCR products were separated on 1% agarose gel to verify the appropriate size.…”

Section: Methodsmentioning

confidence: 99%

High-Throughput Sequencing Facilitates Discovery of New Plant Viruses in Poland

Minicka

Zarzyńska‐Nowak

Budzyńska

et al. 2020

Plants

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Viruses cause epidemics on all major crops of agronomic importance, and a timely and accurate identification is essential for control. High throughput sequencing (HTS) is a technology that allows the identification of all viruses without prior knowledge on the targeted pathogens. In this paper, we used HTS technique for the detection and identification of different viral species occurring in single and mixed infections in plants in Poland. We analysed various host plants representing different families. Within the 20 tested samples, we identified a total of 13 different virus species, including those whose presence has not been reported in Poland before: clover yellow mosaic virus (ClYMV) and melandrium yellow fleck virus (MYFV). Due to this new finding, the obtained sequences were compared with others retrieved from GenBank. In addition, cucurbit aphid-borne yellows virus (CABYV) was also detected, and due to the recent occurrence of this virus in Poland, a phylogenetic analysis of these new isolates was performed. The analysis revealed that CABYV population is highly diverse and the Polish isolates of CABYV belong to two different phylogenetic groups. Our results showed that HTS-based technology is a valuable diagnostic tool for the identification of different virus species originating from variable hosts, and can provide rapid information about the spectrum of plant viruses previously not detected in a region.

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