Complete Genome Sequence of Escherichia coli O157:H7 Phage PhiG17

Akindolire, Muyiwa Ajoke; Aremu, Bukola Rhoda; Ateba, Collins Njie

doi:10.1128/mra.01296-18

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…For instance, in West Africa, sequenced genomes of African locust bean (Parkia biglobosa) and bush mango (Cordyla pinnata) could be used for training students in structural genomics to understand genome organization and easily link gene content, adaptability and socio-economic relevance. Second, the classical organisms used for bioinformatics trainings in Africa, for example Escherichia coli or Homo sapiens 18,19 , do not provide a broad repertoire of the challenges-such as difficulty in sequencing or high genetic diversity-that exist in working with nonmodel, endemic species. For instance, nonmodel vertebrates such as African lions and leopards will require unconventional sample materials and sequencing approaches [20][21][22][23][24][25] .…”

Section: Careers and Recruitmentmentioning

confidence: 99%

Bridging the gap in African biodiversity genomics and bioinformatics

Sharaf,

Ndiribe,

Omotoriogun

et al. 2023

Nat Biotechnol

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Section: Careers and Recruitmentmentioning

confidence: 99%

Bridging the gap in African biodiversity genomics and bioinformatics

Sharaf,

Ndiribe,

Omotoriogun

et al. 2023

Nat Biotechnol

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“…Estimated proteins in the genome are more advanced NCBI’s non-redundant GenBank database is annotated by BLAST. 114 The PhiG17 genome has been detected to carry a sum of 78 coding sequences that differ in length from 114 bp to 10,353 bp which also carries one isoleucine tRNA, as estimated by ARAGORN 113 , 115 together with tRNAscanSE. 113 , 116 As determined by ResFinder v3.0, 117 there are no known genes encoding antimicrobial resistance in the genome.…”

Section: Metagenomic Analysismentioning

confidence: 99%

Review of Escherichia coli O157:H7 Prevalence, Pathogenicity, Heavy Metal and Antimicrobial Resistance, African Perspective

Gambushe¹,

Zishiri²,

Zowalaty³

2022

IDR

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Escherichia coli O157:H7 is an important food-borne and water-borne pathogen that causes hemorrhagic colitis and the hemolytic-uremic syndrome in humans and may cause serious morbidity and large outbreaks worldwide. People with bloody diarrhea have an increased risk of developing serious complications such as acute renal failure and neurological damage. The hemolytic-uremic syndrome (HUS) is a serious condition, and up to 50% of HUS patients can develop long-term renal dysfunction or blood pressurerelated complications. Children aged two to six years have an increased risk of developing HUS. Clinical enteropathogenic Escherichia coli (EPEC) infections show fever, vomiting, and diarrhea. The EPEC reservoir is unknown but is suggested to be an asymptomatic or symptomatic child or an asymptomatic adult carrier. Spreading is often through the fecal-oral route. The prevalence of EPEC in infants is low, and EPEC is highly contagious in children. EPEC disease in children tends to be clinically more severe than other diarrheal infections. Some children experience persistent diarrhea that lasts for more than 14 days. Enterotoxigenic Escherichia coli (ETEC) strains are a compelling cause of the problem of diarrheal disease. ETEC strains are a global concern as the bacteria are the leading cause of acute watery diarrhea in children and the leading cause of traveler's diarrhea. It is contagious to children and can cause chronic diarrhea that can affect the development and well-being of children. Infections with diarrheagenic E. coli are more common in African countries. Antimicrobial agents should be avoided in the acute phase of the disease since studies showed that antimicrobial agents may increase the risk of HUS in children. The South African National Veterinary Surveillance and Monitoring Programme for Resistance to Antimicrobial Drugs has reported increased antimicrobial resistance in E. coli. Pathogenic bacterial strains have developed resistance to a variety of antimicrobial agents due to antimicrobial misuse. The induced heavy metal tolerance may also enhance antimicrobial resistance. The prevalence of antimicrobial resistance depends on the type of the antimicrobial agent, bacterial strain, dose, time, and mode of administration. Developing countries are severely affected by increased resistance to antimicrobial agents due to poverty, lack of proper hygiene, and clean water, which can lead to bacterial infections with limited treatment options due to resistance.

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“…E. coli 0157:H7 bacteria were recognized in 1982 for the first time as a cause of illness during an outbreak which caused bloody diarrhea in people who drank contaminated water [6]. According to the report made by Tam and co-workers, 600 million cases of human diarrhea and 800,000 worldwide deaths mainly in children under the age of 5 years were due to E. coli 0157:H7 [7]. According to WHO and national standards when viable cell numbers of E. coli in water range between 100 -1000 CFU/mL, the water is considered to be at intermediate risk and high risk is declared when the E. coli cells range between 100 -1000 CFU/mL.…”

Section: Introductionmentioning

confidence: 99%

Musa Paradaisica and <i>Vitis vinifera</i> Functionalised Ag-NPs: Electrochemical and Optical Detection of <i>Escherichia coli</i> in Seawater

Nqunqa¹,

Muluadzi²,

Njomo³

et al. 2022

JSEMAT

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Herein, we demonstrate a simple and inexpensive one-pot green synthesis of silver nanoparticles (Ag-NPs) functionalised with a combination of banana peel (Musa paradisiaca) and grape (Vitis vinifera) fruit extracts. The reaction mixture of aqueous silver nitrate, banana peel and grapefruit extracts revealed a dark brown colour after a reaction time of 18 minutes, which indicates the presence and the successful synthesis of silver nanoparticles. The optical and structural properties of the green synthesised nanoparticles were analysed using UV-Visible spectroscopy (UV-Vis) which confirmed an absorption band at 440 nm. The polydispersity nature and the AgNPs sizes of 30 nm were revealed using small angle X-ray scattering (SAXS) and high-resolution transmission electron microscopy (HR-TEM) techniques. Fourier transform infrared spectroscopy (FT-IR) studies revealed the structure of these nanoparticles which included carbonyl groups, primary amine groups, OH groups and other stabilizing functional groups characteristic of the properties of combined extracts. A simple, quick, less time-consuming surface plasmon resonance (SPR) and electrochemical method in the form of optical and electrochemical sensors have been developed for the detection of Escherichia coli 0157:H7. The obtained limit of detection (LOD) values for SPR and GBPE-Ag-NPs/GCE-based sensor systems were found to be 1 × 102 CFU/mL and 3.5 × 101 CFU/mL, respectively. The obtained values fall within the range

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Complete Genome Sequence of Escherichia coli O157:H7 Phage PhiG17

Cited by 9 publications

References 20 publications

Bridging the gap in African biodiversity genomics and bioinformatics

Bridging the gap in African biodiversity genomics and bioinformatics

Review of Escherichia coli O157:H7 Prevalence, Pathogenicity, Heavy Metal and Antimicrobial Resistance, African Perspective

Musa Paradaisica and <i>Vitis vinifera</i> Functionalised Ag-NPs: Electrochemical and Optical Detection of <i>Escherichia coli</i> in Seawater

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Complete Genome Sequence of Escherichia coli O157:H7 Phage PhiG17

Cited by 9 publications

References 20 publications

Bridging the gap in African biodiversity genomics and bioinformatics

Bridging the gap in African biodiversity genomics and bioinformatics

Review of Escherichia coli O157:H7 Prevalence, Pathogenicity, Heavy Metal and Antimicrobial Resistance, African Perspective

Musa Paradaisica and &lt;i&gt;Vitis vinifera&lt;/i&gt; Functionalised Ag-NPs: Electrochemical and Optical Detection of &lt;i&gt;Escherichia coli&lt;/i&gt; in Seawater

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Musa Paradaisica and <i>Vitis vinifera</i> Functionalised Ag-NPs: Electrochemical and Optical Detection of <i>Escherichia coli</i> in Seawater