2014
DOI: 10.1128/genomea.00889-14
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Complete Genome Sequence of Flavobacterium psychrophilum Strain CSF259-93, Used To Select Rainbow Trout for Increased Genetic Resistance against Bacterial Cold Water Disease

Abstract: The genome sequence of Flavobacterium psychrophilum strain CSF259-93, isolated from rainbow trout (Oncorhynchus mykiss), consists of a single circular genome of 2,900,735 bp and 2,701 predicted open reading frames (ORFs). Strain CSF259-93 has been used to select a line of rainbow trout with increased genetic resistance against bacterial cold water disease.

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Cited by 25 publications
(24 citation statements)
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“…Bacterial challenge was carried out in the NCCCWA challenge facility with F. psychrophilum strain CSF-259-93 (initially provided by Dr. S. LaPatra, Clear Springs Foods, Inc.). This strain was previously isolated from a BCWD field-case and maintained at −80°C in TYES media supplemented with 10% (v/v) glycerol and has been consistently utilized as the challenge strain within the selective breeding program (Hadidi et al, 2008 ; Silverstein et al, 2009 ; Leeds et al, 2010 ; Wiens et al, 2013a ) and the complete genome sequence determined (Wiens et al, 2014 ). Frozen stock was cultivated on TYES media for 5 days at 15°C, suspended in PBS and O.D.…”
Section: Methodsmentioning
confidence: 99%
“…Bacterial challenge was carried out in the NCCCWA challenge facility with F. psychrophilum strain CSF-259-93 (initially provided by Dr. S. LaPatra, Clear Springs Foods, Inc.). This strain was previously isolated from a BCWD field-case and maintained at −80°C in TYES media supplemented with 10% (v/v) glycerol and has been consistently utilized as the challenge strain within the selective breeding program (Hadidi et al, 2008 ; Silverstein et al, 2009 ; Leeds et al, 2010 ; Wiens et al, 2013a ) and the complete genome sequence determined (Wiens et al, 2014 ). Frozen stock was cultivated on TYES media for 5 days at 15°C, suspended in PBS and O.D.…”
Section: Methodsmentioning
confidence: 99%
“…Water quality parameters at the NCCCWA have been described previously [38]. Fish were challenged by intraperitoneal injection with 500 L of PBS alone, or PBS containing 2.3 ± 0.4 x 10 8 CFU of the genomesequenced strain of F. psychrophilum CSF259-93 [40]. During the experimental challenge period, one ARS-Fp-S line fish died on day 4 and Fp was confirmed by plate culture using TYES media.…”
Section: Samplingmentioning
confidence: 99%
“…BCWD has a significant economic impact on aquaculture operations in the United States and Canada. The genome sequences of the F. psychrophilum vaccine strain ( 5 ), type strain ( 6 ), and a European isolate are available ( 7 ). Here, we report the sequencing of three BCWD outbreak-associated F. psychrophilum strains isolated from rainbow trout ( Oncorhynchus mykiss ) from three different hatcheries in the United States.…”
Section: Genome Announcementmentioning
confidence: 99%