Complete genome sequence of Kosakonia sacchari type strain SP1T

Chen, Mingyue; Zhu, Bo; Lin, Li; Yang, Li‐Tao; Li, Yang–Rui; An, Qianli

doi:10.4056/sigs.5779977

Cited by 36 publications

(26 citation statements)

References 19 publications

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“…The increasing cell densities observed between the different root regions indicate that K. sacchari proliferated along the growing root. The strain used in this study is very closely related to type strain K. sacchari SP1 T (99.89% 16S rRNA sequence identity, Chen et al, 2014), a heterotrophic bacterium that has been reported to colonize sugarcane plants and to exhibit plantgrowth promotion through nitrogen fixation. In this study, the dense colonization of all observed regions indicates that carbon substrates provided by the plant (i.e., the necessary food source for K. sacchari in our experimental system) may not only have been available at the root tip but also at the elongation zone, as well was in the zone of maturity.…”

Section: Bacterial Cell Distributions On Rootsmentioning

confidence: 99%

Recognizing Patterns: Spatial Analysis of Observed Microbial Colonization on Root Surfaces

Schmidt

Nunan

Hock

et al. 2018

Front. Environ. Sci.

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Root surfaces are major sites of interactions between plants and associated microorganisms. Here, plants and microbes communicate via signaling molecules, compete for nutrients, and release substrates that may have beneficial or harmful effects on each other. Whilst the body of knowledge on the abundance and diversity of microbial communities at root-soil interfaces is now substantial, information on their spatial distribution at the microscale is still scarce. In this study, a standardized method for recognizing and analyzing microbial cell distributions on root surfaces is presented. Fluorescence microscopy was combined with automated image analysis and spatial statistics to explore the distribution of bacterial colonization patterns on rhizoplanes of rice roots. To test and evaluate the presented approach, a gnotobiotic experiment was performed using a potential nitrogen-fixing bacterial strain in combination with roots of wetland rice. The automated analysis procedure resulted in reliable spatial data of bacterial cells colonizing the rhizoplane. Among all replicate roots, the analysis revealed an increasing density of bacterial cells from the root tip to the region of root cell maturation. Moreover, bacterial cells showed significant spatial clustering and tended to be located around plant root cell borders. The quantitative data suggest that the structure of the root surface plays a major role in bacterial colonization patterns. Possible adaptations of the presented approach for future studies are discussed along with potential pitfalls such as inaccurate imaging. Our results demonstrate that standardized recognition and statistical evaluation of microbial colonization on root surfaces holds the potential to increase our understanding of microbial associations with roots and of the underlying ecological interactions.

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Section: Bacterial Cell Distributions On Rootsmentioning

confidence: 99%

Recognizing Patterns: Spatial Analysis of Observed Microbial Colonization on Root Surfaces

Schmidt

Nunan

Hock

et al. 2018

Front. Environ. Sci.

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“…Kosakonia sacchari (formerly known as Enterobacter sacchari) is a Gram-negative aerobic diazotrophic bacterium belonging to the class of Gammaproteobacteria [27]. An endo polygalacturonase gene was reported in the complete genome sequence of K. sacchari strain BO-1 (NZ_CP016337.1).…”

Section: Discussionmentioning

confidence: 99%

LC/MS detection of oligogalacturonic acids obtained from tragacanth degradation by pectinase producing bacteria

et al. 2018

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Tragacanth, a highly branched carbohydrate polymer isolated from Astragalus, is one of the most commonly used gums in food industry. The primary structure of tragacanth is composed of galacturonic acid monomers connected with α 1-4 links, and it is very similar to the pectin. Tragacanth degradation by microorganisms is significant in two aspects: first, food preservation and microbial growth control due to too much use of tragacanth in the food industry, second, therapeutic and pharmaceutical potential of obtained oligosaccharides. In the present study, we report three new strains of bacteria, Acinetobacter guillouiae strain TD1, Kosakonia sacchari strain TD2, and Bacillus vallismortis strain PD1 with the capability of growing in tragacanth as an only source of carbon and energy. The evolutionary history of the isolated strains was analyzed based on 16S rRNA gene sequences in MEGA7 using the neighbor-joining method. The production of di and tri galacturonic acid due to pectinase activities of the strains were detected by thin layer chromatography (TLC) and liquid chromatography/ Mass spectroscopy (LC/MS) analysis. Here is the first report of the ability to grow in tragacanth and pectinase activity monitoring in bacteria. Our results revealed that all of the isolated strains are capable of degrading pectin and tragacanth to oligo-galacturonic acids. The obtained products, which have different structures depending on the tragacanth structures and types of pectinolytic enzymes, would show therapeutic and pharmaceutical potentials. K E Y W O R D SAcinetobacter guillouiae, Bacillus vallismortis, Kosakonia sacchari, pectinase, tragacanth

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“…Kosakonia sacchari was isolated, identified and proved to cause potato soft rot [18]. On the other hand, Kosakonia sacchari was isolated as a nitrogen fixing bacteria that colonize sugar cane promoting its growth [26].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

Ea¹,

AlKhzindar²,

Eta³

2018

J Bacteriol Parasitol

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Potato crop is one of the most economically important crops, and is considered as the fourth main food crop in the world. One of the most important serious diseases of potato is bacterial soft rot disease. In our study, soil samples were collected from potato rhizosphere from a potato cultivated field in Giza, Egypt. Two bacteriophages were isolated on Kosakonia sacchari as a host, causing potato soft rot disease. Based on the genome characterization and electron microscopy, the two phages were named as vB_KsaM-C1 belonging to Myoviridae and vB_KsaO-C2 belonging to Microviridae. Both phages sustained their activity more than 20 months with a remarkable decrease in phage titre. In addition both recorded the same TIP at 65°C and showed the optimum activity at pH 7. The DEP of vB_KsaM-C1 was 10-7, while that of phage vB_KsaO-C2 was 10-9. A small scale application of the two isolated bacteriophages on infected potato tubers discs, in the lab, successfully inhibited bacterial soft rot caused by Kosakonia sacchari.

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Complete genome sequence of Kosakonia sacchari type strain SP1T

Cited by 36 publications

References 19 publications

Recognizing Patterns: Spatial Analysis of Observed Microbial Colonization on Root Surfaces

Recognizing Patterns: Spatial Analysis of Observed Microbial Colonization on Root Surfaces

LC/MS detection of oligogalacturonic acids obtained from tragacanth degradation by pectinase producing bacteria

Isolation and Characterization of Two Bacteriophages Infecting Kosakonia sacchari Bacterium Causing Potato Soft Rot Disease

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