Complete genome sequence of Mycobacterium goodii X7B, a facultative thermophilic biodesulfurizing bacterium with industrial potential

Yu, Bo; Tao, Fei; Li, Fu‐Li; Hou, Jianfeng; Tang, Hongzhi; Ma, Cuiqing; Xu, Ping

doi:10.1016/j.jbiotec.2015.08.004

Cited by 12 publications

(4 citation statements)

References 10 publications

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“…Among the numerous biodesulfurization cultures isolated, there are several that possess an extended 4S pathway such that 2-HPB is not the end product of DBT desulfurization, and instead 2-methoxybiphenyl (2-MBP) [38][39][40], biphenyl [41], or 2,2-dihydroxybiphenyl [42] are produced. The toxicity of 2-MBP is reported to be only slightly less than 2-HBP [43], but this study examined the inhibition of DBT biodesulfurization by externally added 2-MBP or 2-HBP and did not address the intracellular accumulation of 2-HBP [44].…”

Section: Discussionmentioning

confidence: 99%

Desulfurization of Dibenzothiophene by Pseudomonas fluorescens (UCP 1514) Leading to the Production of Biphenyl

Silva¹,

Schwartz²,

Souza³

et al. 2018

Recent Insights in Petroleum Science and Engineering

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Dibenzothiophene (DBT) is a typical recalcitrant thiophenic sulfur component of fuels, and its desulphurization has been a model reaction in the treatment of these compounds. Based on this information, the potential of Pseudomonas fluorescens (UCP 1514) on the desulfurization of dibenzothiphene was studied, in order to use it for reducing the sulfur content of diesel oil in compliance with environmental regulations. The result of biodegradation by the bacteria was determined by undertaking high-performance liquid chromatography of the metabolites produced. These can also be identified by gas chromatography with a mass spectrometry detector, and doing so revealed a sulfur-free product, biphenyl, as the final product of the degradation process. The results showed a decrease of 73% in dibenzothiophene content, which means that P. fluorescens removes sulfur from dibenzothiophene with a good selectivity to form biphenyl. These promising results indicate that P. fluorescens has an interesting potential to degrade sulfur-containing compounds in diesel oil and thereby could help in removing sulfur content from diesel oil. The process of microbial desulfurization described herein can be used particularly after carrying out hydrodesulfurization. Consequently, the sulfur content could be reduced even further. Applying P. fluorescens UCP 1514 in dibenzothiophene could help to understand the nature of the biodegradation process and to achieve the regulatory standards for sulfur level in fossil fuels.

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Section: Discussionmentioning

confidence: 99%

Desulfurization of Dibenzothiophene by Pseudomonas fluorescens (UCP 1514) Leading to the Production of Biphenyl

Silva¹,

Schwartz²,

Souza³

et al. 2018

Recent Insights in Petroleum Science and Engineering

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“…FRs catalyze the reduction of free flavins (riboflavin, FMN, or FAD) by using reduced pyridine nucleotides, NADPH, or NADH. MgFR from Mycobacterium goodii X7B [26] showed strict specificity toward NADH with high enzyme activity (678 U/mg), and thus was tested for the regeneration of NAD + in the Bs-7α-HSDH-catalyzed oxidation of CDCA. E. coli (BsMg) cells co-expressing Bs-7α-HSDH and MgFR were used as biocatalyst in the following studies ( Figure S9).…”

Section: Flavin Oxidoreductase-catalyzed Regeneration Of Nad + For Thmentioning

confidence: 99%

“…However, until now FR/FMN system has not been used to regenerate the cofactor NAD + or NADP + . Flavin oxidoreductase (MgFR) from Mycobacterium goodii X7B [26] showed 678 U/mg (pH 7.0) specific activity toward the hydride transfer from NADH to FMN, [24] yielding NAD + and the reduced FMN (FMNH 2 ). [27] The fast autoxidation of FMNH 2 [28] suggests that it may be not necessary to use the stoichiometric amount of FMN.…”

Section: Introductionmentioning

confidence: 99%

Flavin Oxidoreductase‐Mediated Regeneration of Nicotinamide Adenine Dinucleotide with Dioxygen and Catalytic Amount of Flavin Mononucleotide for One‐Pot Multi‐Enzymatic Preparation of Ursodeoxycholic Acid

et al. 2019

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Ursodeoxycholic acid (UDCA), a pharmaceutical ingredient widely used in clinics, can be prepared from chenodeoxycholic acid (CDCA) by the epimerization of the 7α-OH group. In this study, a nicotinamide adenine dinucleotide (NAD + ) regeneration system was developed by using flavin oxidoreductase (FR) and flavin mononucleotide (FMN). Only catalytic amount of FMN is required for the effective NAD + recycling. FR/FMN system was then applied in the oxidation of CDCA to 7-ketolithocholic acid (7-keto-LCA) by NAD + -dependent 7α-hydroxysteroid dehydrogenase (Bs-7α-HSDH) from Brevundimonas sp., which showed extremely high enzyme activity toward CDCA (k cat /K m = 8050 s À 1 · mM À 1 ). When Escherichia coli whole cells coexpressing Bs-7α-HSDH and FR genes were used as biocatalyst, CDCA (50 mM) was completely converted to 7-keto-LCA with the turnover number of FMN being 227 and 58.8 g · L À 1 · d À 1 space-time yield of 7-keto-LCA. For the reduction of 7-keto-LCA, nicotinamide adenine dinucleotide phosphate (NADPH)-dependent 7-β-hydroxysteroid dehydrogenase (Cm-7β-HSDH) from Clostridium sp. Marseille was employed with alcohol dehydrogenase from Thermoanaerobacter brockii (TbADH) and iso-propanol as co-factor regeneration system. When E. coli whole cells coexpressing Cm-7β-HSDH and TbADH genes were used as biocatalyst, 40 mM 7-keto-LCA was reduced to UDCA with 26.8 g · L À 1 · d À 1 space-time yield. The oxidation and reduction were then carried in a one-pot concurrent mode, 12.5 mM CDCA was completely converted to UDCA. The epimerization of CDCA to UDCA proceeded to completion at the substrate concentration of 30 mM in the one-pot sequential process. Therefore, the complete conversion of CDCA to UDCA in one-pot has been realized by employing 7α-HSDH and 7β-HSDH of different co-factor specificities with independent co-factor recycling systems. The cholic acids, especially UDCA, exert inhibitive effect on the activities of these enzymes, preventing the complete epimerization of 7α-OH at higher substrate loading. This inhibition issue should be solvable by engineering the involved enzymes, that is currently pursued in our laboratory.

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“…Although HDS can effectively remove thiols, sulfides, and disulfides from the fuels, it is less efficient in removing aromatic sulfur-containing compounds such as dibenzothiophene (DBT) and its derivatives. Moreover, requiring severe operating conditions such as high temperature and high hydrogen pressure is another disadvantage of this process. , Therefore, many other desulfurization methods having mild operations, e.g., biodesulfurization, , adsorption, − extraction, − and oxidative desulfurization (ODS), − have been studied. Oxidative desulfurization is regarded as one of the most promising methods in deep desulfurization of fuels under mild conditions.…”

Section: Introductionmentioning

confidence: 99%

Oxidative Desulfurization of Diesel Fuel Using a Brønsted Acidic Ionic Liquid Supported on Silica Gel

et al. 2017

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A novel heterogeneous catalyst was prepared by supporting the ionic liquid (IL) 1-octyl-3-methylimidazolium hydrogen sulfate ([Omim][HSO4]) in the silica-gel matrix using a sol–gel method and employed in oxidative desulfurization of a model oil containing dibenzothiophene (DBT) and real diesel fuel. The properties of the supported ionic liquid (SIL) were characterized by FT-IR, TGA, BET-BJH, SEM-EDS, and PSA. The results showed that the ionic liquid was successfully incorporated in the silica-gel matrix, and a catalyst having high surface area was prepared. The effects of H2O2/DBT molar ratio (O/S), temperature, SIL/oil mass ratio, initial S content, and sulfur species on the sulfur removal of the model oil were studied. The highest DBT removal efficiency of 99.1% was obtained with the prepared catalyst having 17 wt % supported IL loading in the conditions of SIL/oil (w/w) of 1:3 and O/S molar ratio of 5 in 50 min at 50 °C. The prepared catalyst can be efficiently applied in oxidative desulfurization with consumption of much lower IL compared to that in a desulfurization system using bulk IL. The order of oxidative removal efficiencies of different sulfur species is as follows: DBT > BT > TS > 4,6-DMDBT. The SIL can be separated by a simple filtration from the reaction system and reused four times without any significant decrease in its performance. A high sulfur removal of 75.7% was also obtained by applying the prepared catalyst in desulfurization of the hydrotreated real diesel fuel.

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Complete genome sequence of Mycobacterium goodii X7B, a facultative thermophilic biodesulfurizing bacterium with industrial potential

Cited by 12 publications

References 10 publications

Desulfurization of Dibenzothiophene by Pseudomonas fluorescens (UCP 1514) Leading to the Production of Biphenyl

Desulfurization of Dibenzothiophene by Pseudomonas fluorescens (UCP 1514) Leading to the Production of Biphenyl

Flavin Oxidoreductase‐Mediated Regeneration of Nicotinamide Adenine Dinucleotide with Dioxygen and Catalytic Amount of Flavin Mononucleotide for One‐Pot Multi‐Enzymatic Preparation of Ursodeoxycholic Acid

Oxidative Desulfurization of Diesel Fuel Using a Brønsted Acidic Ionic Liquid Supported on Silica Gel

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