Complete Genome Sequence of Staphylococcus aureus Strain Wood 46

Balachandran, Manasi; Riley, Matthew C.; Bemis, David A.; Kania, Stephen A.

doi:10.1128/genomea.00087-17

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…We used Wood46 as a model strain for PNAG-positive biofilm because strain Wood46 is known to produce PNAG ( O’Brien et al, 2001 ) and known for its low surface expression of IgG binding staphylococcal protein A (SpA). This is an advantage in antibody binding assays because nonspecific binding of the IgG1 Fc-domain to SpA complicates the detection of antibodies ( Amend et al, 1984 ; Balachandran et al, 2017b ). As a model strain for PNAG-negative biofilms, we used LAC ( Kennedy et al, 2008 ; Voyich et al, 2005 ; Montgomery et al, 2008 ), a member of the USA300 lineage that has emerged as the common cause of healthcare-associated MRSA infections, including implant infections ( Seybold et al, 2006 ; Carrel et al, 2015 ; See et al, 2020 ; Haque et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

“…S. aureus strains Wood46 (ATCC 10832) ( Amend et al, 1984 ; Balachandran et al, 2017b ; Balachandran et al, 2017a ), USA300 LAC (AH1263) ( Boles et al, 2010 ), and USA300 LAC ∆spa, sbi ::Tn (AH4116) were used in this study. Strain USA300 LAC ∆spa, sbi ::Tn (AH4116) was constructed by transducing sbi ::Tn from Nebraska Transposon Library ( Fey et al, 2013 ) into USA300 LAC ∆spa (AH3052) ( Ibberson et al, 2014 ) with phage 11.…”

Section: Methodsmentioning

confidence: 99%

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Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

Vor

Dijk

Kessel

et al. 2022

eLife

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Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and potential treatment of biofilm-related infections. Here, we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. The mAbs were also shown to bind a collection of clinical isolates derived from different biofilm-associated infections (endocarditis, prosthetic joint, catheter). We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. Furthermore, we show that a mAb recognizing wall teichoic acid (clone 4497) specifically localizes to a subcutaneously implanted pre-colonized catheter in mice. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

Vor

Dijk

Kessel

et al. 2022

eLife

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show abstract

“…This is consistent with the fact that Wood46 is known to produce PNAG (45). An extra advantage of Wood46 is its low surface expression of IgG binding Staphylococcal protein A (SpA), which complicates the detection of antibodies(46, 47). As a model strain for PNAG-negative biofilms we used LAC (48–50), a member of the USA300 lineage which has emerged as the common cause of healthcare-associated MRSA infections, including implant infections (51–54).…”

Section: Resultsmentioning

confidence: 99%

“…S. aureus strains Wood46 (ATCC 10832) (46, 47, 77), USA300 LAC (AH1263) (29) and USA300 LAC Δ spa, sbi ∷Tn (AH4116) were used in this study. Strain USA300 LAC Δ spa, sbi ∷Tn (AH4116) was constructed by transducing sbi ∷Tn from Nebraska Transposon Library (78) into USA300 LAC Δ spa (AH3052) (79) with phage 11.…”

Section: Methodsmentioning

confidence: 99%

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

Vor

Kessel

et al. 2021

Preprint

View full text Add to dashboard Cite

Implant-associated Staphylococcus aureus infections are difficult to treat because of biofilm formation. Bacteria in a biofilm are often insensitive to antibiotics and host immunity. Monoclonal antibodies (mAbs) could provide an alternative approach to improve the diagnosis and/or treatment of biofilm-related infections. Here we show that mAbs targeting common surface components of S. aureus can recognize clinically relevant biofilm types. We identify two groups of antibodies: one group that uniquely binds S. aureus in biofilm state and one that recognizes S. aureus in both biofilm and planktonic state. In a mouse model, we show that mAb 4497 (recognizing wall teichoic acid (WTA)) specifically localizes to biofilm-infected implants. In conclusion, we demonstrate the capacity of several human mAbs to detect S. aureus biofilms in vitro and in vivo. This is an important first step to develop mAbs for imaging or treating S. aureus biofilms.

show abstract

Complete Genome Sequence of Staphylococcus aureus Strain Wood 46

Cited by 2 publications

References 15 publications

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm

Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

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