Complete Genome Sequence of Staphylococcus aureus M013, a pvl-Positive, ST59-SCCmec Type V Strain Isolated in Taiwan

Huang, Tsung-Hui; Chen, Feng-Jui; Miu, Wei-Chieh; Liao, Tsai-Lien; Lin, Ann-Chi; Huang, I-Wen; Kk, Wu; Tsai, Shih‐Feng; Chen, Ying-Tsong; Lauderdale, Tsai‐Ling

doi:10.1128/jb.06666-11

Cited by 29 publications

(26 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lastly, the complete genome sequence of an ST59 CA-MRSA isolate, a lineage predominant in Taiwan, was reported recently (Huang et al, 2012a, 2012b). Since the original identification of ST59, these isolates have frequently been associated with multi-drug resistance.…”

Section: Community-associated Mrsamentioning

confidence: 99%

“…Since the original identification of ST59, these isolates have frequently been associated with multi-drug resistance. Compared to the USA300 strain FPR3757 and the USA4000 strain MW2, most of the open reading frames in the ST59 strain M013 were highly conserved (Huang et al 2012a). However, genomic islands SaPI5 and νSa3 as well as prophages ϕSa3usa and ϕSa3 are absent in M013 (Huang et al, 2012a, 2012b).…”

Section: Community-associated Mrsamentioning

confidence: 99%

See 1 more Smart Citation

Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus

Uhlemann

Otto

Lowy

et al. 2014

Infection, Genetics and Evolution

164

166

View full text Add to dashboard Cite

Staphylococcus aureus is a prominent cause of human infections globally. The high prevalence of infections is compounded by antibiotic resistance—a significant problem for treatment. Methicillin-resistant S. aureus (MRSA) is endemic in hospitals and healthcare facilities worldwide, and is an increasingly common cause of community-associated bacterial infections in industrialized countries. Although much focus is placed on the role of S. aureus as a human pathogen, it is in fact a human commensal organism that has had a relatively long coexistence with the human host. Many S. aureus infections can be explained by host susceptibility or other predisposing risk factors. On the other hand, the emergence/re-emergence of successful S. aureus clones (referred to as epidemic waves) suggests a rapid bacterial adaption and evolution, which includes the emergence of antibiotic resistance and increased virulence and/or transmissibility. It is within this context that we review our understanding of selected S. aureus epidemic waves, and highlight the use of genome sequencing as a means to better understand the evolution of each lineage.

show abstract

Section: Community-associated Mrsamentioning

confidence: 99%

Section: Community-associated Mrsamentioning

confidence: 99%

Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus

Uhlemann

Otto

Lowy

et al. 2014

Infection, Genetics and Evolution

164

166

View full text Add to dashboard Cite

show abstract

“…However, an ST59 community-associated MRSA clinical Taiwanese strain, M013 (43), which shares three cmk synonymous and cmk(S203T) mutations with the two ST45 strains, is susceptible to vancomycin (MIC, Յ0.5 mg/liter) (T. L. Lauderdale, personal communication). Therefore, it is not clear if cmk(S203T) is directly associated with raised vancomycin resistance or not.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Identification of Mutations Responsible for Heterogeneous Vancomycin-Intermediate Staphylococcus aureus (hVISA)-to-VISA Conversion in Laboratory-Generated VISA Strains Derived from hVISA Clinical Strain Mu3

Matsuo

Cui

Kim

et al. 2013

Antimicrob Agents Chemother

View full text Add to dashboard Cite

bHeterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) spontaneously produces VISA cells within its cell population at a frequency of 10 ؊6 or greater. We established a total of 45 VISA mutant strains independently obtained from hVISA Mu3 and its related strains by one-step vancomycin selection. We then performed high-throughput whole-genome sequencing of the 45 strains and their parent strains to identify the genes involved in the hVISA-to-VISA phenotypic conversion. A comparative genome study showed that all the VISA strains tested carried a unique set of mutations. All of the 45 VISA strains carried 1 to 4 mutations possibly affecting the expression of a total of 48 genes. Among them, 32 VISA strains carried only one gene affected by a single mutation. As many as 20 genes in more than eight functional categories were affected in the 32 VISA strains, which explained the extremely high rates of the hVISA-to-VISA phenotypic conversion. Five genes, rpoB, rpoC, walK, pbp4, and pp2c, were previously reported as being involved in vancomycin resistance. Fifteen remaining genes were newly identified as associated with vancomycin resistance in this study. The gene most frequently affected (6 out of 32 strains) was cmk, which encodes cytidylate kinase, followed closely by rpoB (5 out of 32), encoding the ␤ subunit of RNA polymerase. A mutation prevalence study also revealed a sizable number of cmk mutants among clinical VISA strains (7 out of 38 [18%]). Reduced cytidylate kinase activity in cmk mutant strains is proposed to contribute to the hVISA-to-VISA phenotype conversion by thickening the cell wall and reducing the cell growth rate.

show abstract

“…To broaden our survey, we analyzed cell-free culture extracts of 12 additional S. aureus strains or clinical isolates ( Table S1) . N-AgrD peptides were produced by three USA300 lineage MRSA, two ST59 MRSA (Huang et al, 2012), and clonal colony ST45 of USA600 MRSA lineage ( Figure 1C ). In successive MS experiments, peptide sequences most abundantly mapped to AgrD (McIlwain et al, 2012), herein designated (i) N-AgrD F20, (ii) N-AgrD F24, and (iii) N-AgrD D20, were synthesized and isolated at >95% purity for functional assessment.…”

Section: Resultsmentioning

confidence: 99%

N-Terminal ArgD Peptides from the Classical Staphylococcus aureus Agr System Have Cytotoxic and Proinflammatory Activities

Gonzalez¹,

Corriden²,

Akong-Moore³

et al. 2014

Chemistry & Biology

View full text Add to dashboard Cite

SUMMARY AgrD is the precursor for the auto-inducing peptide in a quorum sensing system regulating virulence phenotypes of the pre-eminent pathogen Staphylococcus aureus. Mass spectrometry-based methods, including molecular networking, identified formylated and non-formylated peptide variants derived from the AgrD N-terminal leader domain in S. aureus cell-free culture supernatants. Functional assessment of these peptides revealed the unexpected bioactivities including human cell-line cytotoxicity, modulation of neutrophil chemotaxis, neutrophil extracellular trap formation, and the aggravation of skin lesions in vivo.

show abstract

Complete Genome Sequence of Staphylococcus aureus M013, a pvl-Positive, ST59-SCCmec Type V Strain Isolated in Taiwan

Cited by 29 publications

References 16 publications

Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus

Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus

Comprehensive Identification of Mutations Responsible for Heterogeneous Vancomycin-Intermediate Staphylococcus aureus (hVISA)-to-VISA Conversion in Laboratory-Generated VISA Strains Derived from hVISA Clinical Strain Mu3

N-Terminal ArgD Peptides from the Classical Staphylococcus aureus Agr System Have Cytotoxic and Proinflammatory Activities

Contact Info

Product

Resources

About