Complete Genome Sequence of Streptococcus suis Serotype 14 Strain JS14

Hu, Pan; Yang, Ming; Zhang, Anding; Wu, Jiayan; Chen, Bin; Hua, Yafeng; Yu, Jun; Xiao, Jingfa; Jin, Meilin

doi:10.1128/jb.00083-11

Cited by 33 publications

(30 citation statements)

References 21 publications

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“…b Data are from assays using tetracycline (10 g/ml) for resistance selection, but closely comparable results were obtained using erythromycin (10 g/ml). (16,25). The tandem presence of a ribosomal protection tet gene and an efflux tet gene is consistent with the high-level tetracycline resistance displayed by S. suis 32457 and the transconjugants.…”

Section: Evidence Of a 50-to 60-kb Mobile Element Carrying Tet(o/w/ 3mentioning

confidence: 54%

Characterization of a Streptococcus suis tet(O/W/32/O)-Carrying Element Transferable to Major Streptococcal Pathogens

Palmieri

Magi

Mingoia

et al. 2012

Antimicrob Agents Chemother

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Mosaic tetracycline resistance determinants are a recently discovered class of hybrids of ribosomal protection tet genes. They may show different patterns of mosaicism, but their final size has remained unaltered. Initially thought to be confined to a small group of anaerobic bacteria, mosaic tet genes were then found to be widespread. In the genus Streptococcus, a mosaic tet gene identified). The novel element, which we designated 15K, also carried the macrolide resistance determinant erm(B) and an aminoglycoside resistance four-gene cluster including aadE (streptomycin) and aphA (kanamycin). 15K appeared to be an unstable genetic element that, in the absence of recombinases, is capable of undergoing spontaneous excision under standard growth conditions. In the integrated form, 15K was found inside a 54,879-bp integrative and conjugative element (ICE) (50.5% G؉C; 55 ORFs), which we designated ICESsu32457. An ϳ1.3-kb segment that apparently served as the att site for excision of the unstable 15K element was identified. The novel ICE was transferable at high frequency to recipients from pathogenic Streptococcus species (S. suis, Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae), suggesting that the multiresistance 15K element can successfully spread within streptococcal populations.

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Section: Evidence Of a 50-to 60-kb Mobile Element Carrying Tet(o/w/ 3mentioning

confidence: 54%

Characterization of a Streptococcus suis tet(O/W/32/O)-Carrying Element Transferable to Major Streptococcal Pathogens

Palmieri

Magi

Mingoia

et al. 2012

Antimicrob Agents Chemother

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“…A correlation was tentatively established between the CPS structure determined in this study and 7 genes encoding putative glycosyltransferases and a polymerase, which are clustered in the S. suis serotype 14 cps locus (Hu et al 2011;Wang et al 2011) (see Supplementary data Table S1 and Fig. 5).…”

Section: Biosynthesis Of the Capsular Polysaccharidementioning

confidence: 96%

“…Sialic acid has been shown to be an important virulence factor for other meningitis-causing bacteria (Severi et al 2007). The complete genome sequence of this serotype has recently been published (Hu et al 2011). We report here for the first time the structure determination of S. suis serotype 14 CPS using chemical, chromatographic, and spectroscopic methods.…”

Section: Introductionmentioning

confidence: 98%

Structure determination ofStreptococcus suisserotype 14 capsular polysaccharide

Calsteren

Gagnon

Calzas

et al. 2013

Biochem. Cell Biol.

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The capsular polysaccharide (CPS) of Streptococcus suis serotype 14 was purified, chemically modified, and characterized. Sugar and absolute configuration analyses gave the following CPS composition: D-Gal, 3; D-Glc, 1; D-GlcNAc, 1; D-Neu5Ac, 1. The Sambucus nigra lectin, which recognizes the Neu5Ac(α2-6)Gal/GalNAc sequence, showed binding to the native CPS. Sialic acid was found to be terminal, and the CPS was quantitatively desialylated by mild acid hydrolysis. It was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the native CPS or of its specifically modified products allowed to determine the repeating unit sequence: [6)[Neu5Ac(α2-6)Gal(β1-4)GlcNAc(β1-3)]Gal(β1-3)Gal(β1-4)Glc(β1-](n). S. suis serotype 14 CPS has an identical sialic acid-containing side chain as serotype 2 CPS, but differs by the absence of rhamnose in its composition. The same side chain is also present in group B Streptococcus type Ia CPS, except that in the latter sialic acid is 2,3- rather than 2,6-linked to the following galactose. A correlation between the S. suis CPS sequence and genes of the serotype 14 cps locus encoding putative glycosyltransferases and polymerase responsible for the biosynthesis of the repeating unit is proposed.

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“…In serotypes 6,11,12,13,15,17,18,20,21,22,24,27,28,29,30, and 31, partial sequences of the cps gene clusters (orfY-cpsD genes) have been determined and deposited in the GenBank database (accession numbers JF791152 to JF791167). Furthermore, complete genome sequences of 13 S. suis nonreference strains (seven of serotype 2 and one of serotypes 1, 1/2, 3, 7, 9, and 14) have been determined to date (19)(20)(21)(22)(23)(24). A previous study analyzing 15 S. suis cps gene clusters indicated that all the cps gene clusters are located between the orfZ (conserved hypothetical protein gene) and aroA (3-phosphoshikimate 1-carboxyvinyltransferase gene) genes on the chromosome.…”

mentioning

confidence: 99%