Complete Genome Sequence of the Cyanobacterium
            <i>Anabaena</i>
            sp. 33047

Pfeffer, Sarah; Brown, R. Malcolm

doi:10.1128/genomea.00809-16

Cited by 6 publications

(7 citation statements)

References 16 publications

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“…Most cyanobacterial genera have a worldwide distribution and are known to produce cyanotoxins that could pose public health risks, especially if present in springs that are used as spas for therapeutic purposes. However, the cyanobacterium Anabaena has been shown to survive in habitats where fluctuations between saline and freshwater environments occur frequently by accumulating sucrose internally when exposed to saltwater and secreting it into the external milieu when exposed to freshwater (Pfeffer & Brown, ). These sucrose osmolytes are a potential raw material for bioethanol production.…”

Section: Resultsmentioning

confidence: 99%

“…Cyanobacteria are distributed in all types of ecological niches from cold environments to hot springs (Ramganesh et al, 2014) where they constitute important components responsible for the formation of microbial mat tissue with interlaced filaments (Krienitz et al, 2003). shown to survive in habitats where fluctuations between saline and freshwater environments occur frequently by accumulating sucrose internally when exposed to saltwater and secreting it into the external milieu when exposed to freshwater (Pfeffer & Brown, 2016). These sucrose osmolytes are a potential raw material for bioethanol production.…”

Section: Cyanobacteriamentioning

confidence: 99%

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Thermophilic bacterial communities inhabiting the microbial mats of “indifferent” and chalybeate (iron‐rich) thermal springs: Diversity and biotechnological analysis

2017

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Microbial mats are occasionally reported in thermal springs and information on such mats is very scarce. In this study, microbial mats were collected from two hot springs (Brandvlei (BV) and Calitzdorp (CA)), South Africa and subjected to scanning electron microscopy (SEM) and targeted 16S rRNA gene amplicon analysis using Next Generation Sequencing (NGS). Spring water temperature was 55°C for Brandvlei and 58°C for Calitzdorp while the pH of both springs was slightly acidic, with an almost identical pH range (6.2–6.3). NGS analysis resulted in a total of 4943 reads, 517 and 736 OTUs for BV and CA at, respectively, a combined total of 14 different phyla in both samples, 88 genera in CA compared to 45 in BV and 37.64% unclassified sequences in CA compared to 27.32% recorded in BV. Dominant bacterial genera in CA microbial mat were Proteobacteria (29.19%), Bacteroidetes (9.41%), Firmicutes (9.01%), Cyanobacteria (6.89%), Actinobacteria (2.65%), Deinococcus‐Thermus (2.57%), and Planctomycetes (1.94%) while the BV microbial mat was dominated by Bacteroidetes (47.3%), Deinococcus‐Thermus (12.35%), Proteobacteria (7.98%), and Planctomycetes (2.97%). Scanning electron microscopy results showed the presence of microbial filaments possibly resembling cyanobacteria, coccids, rod‐shaped bacteria and diatoms in both microbial mats. Dominant genera that were detected in this study have been linked to different biotechnological applications including hydrocarbon degradation, glycerol fermentation, anoxic‐fermentation, dehalogenation, and biomining processes. Overall, the results of this study exhibited thermophilic bacterial community structures with high diversity in microbial mats, which have a potential for biotechnological exploitation.

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Section: Resultsmentioning

confidence: 99%

Section: Cyanobacteriamentioning

confidence: 99%

Thermophilic bacterial communities inhabiting the microbial mats of “indifferent” and chalybeate (iron‐rich) thermal springs: Diversity and biotechnological analysis

2017

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“…The strain was isolated from the Texas gulf coast more than 5 decades ago. It was then designated Anabaena CA ( 36 , 37 ) For conjugation experiments, cells were grown in BG11 medium with added nitrate, in shake flasks (∼150 rpm), under 150 μmol photons m −2 s −1 of white light in ambient air at 38°C. For physiological studies, cells were grown in ASP2 liquid medium with or without added nitrate at desired light intensities.…”

Section: Methodsmentioning

confidence: 99%

Antenna Modification Leads to Enhanced Nitrogenase Activity in a High Light-Tolerant Cyanobacterium

Bandyopadhyay

Benedikty

et al. 2021

mBio

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“…The strain was isolated from the Texas Gulf coast more than five decades ago. It was then designated as Anabaena CA (36,37) For conjugation experiments, cells were grown in BG11 medium with added nitrate, in shake flasks (~150 rpm), under 150μmol photons m -2 s -1 of white light in ambient air at 38℃. For physiological studies, cells were grown in ASP2 liquid medium with or without added nitrate at desired light intensities.…”

Section: Cyanobacterial Strains and Growth Conditionsmentioning

confidence: 99%

Antenna modification leads to enhanced nitrogenase activity in a high light tolerant cyanobacterium

Bandyopadhyay

Benedikty

et al. 2021

Preprint

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Biological nitrogen fixation is an energy intensive process that contributes significantly towards supporting life on this planet. Among nitrogen-fixing organisms, cyanobacteria remain unrivaled in their ability to fuel the energetically expensive nitrogenase reaction with photosynthetically harnessed solar energy. In heterocystous cyanobacteria light-driven, photosystem I (PSI)-mediated ATP synthesis plays a key role in propelling the nitrogenase reaction. Efficient light transfer to the photosystems rely on phycobilisomes (PBS), the major antenna protein complexes. PBS undergo degradation as a natural response to nitrogen starvation. Upon nitrogen availability, these proteins are resynthesized back to normal levels in vegetative cells, but their occurrence and function in heterocysts remains inconclusive. Anabaena 33047 is a heterocystous cyanobacterium that thrives under high light, harbors higher amounts of PBS in its heterocysts and fixes nitrogen at higher rates compared to other heterocystous cyanobacteria. To assess the relationship between PBS in heterocysts and nitrogenase function, we engineered a strain that retains high amounts of the antenna proteins in its heterocysts. Intriguingly, under high light intensities the engineered strain exhibited unusually high rates of nitrogenase activity compared to the wild type. Spectroscopic analysis revealed altered PSI kinetics in the mutant, with increased cyclic electron flow around PSI, a route that contributes to ATP generation and nitrogenase activity in heterocysts. Retaining higher levels of PBS in heterocysts appears to be an effective strategy to enhance nitrogenase function in cyanobacteria that are equipped with the machinery to operate under high light intensities.ImportanceThe function of phycobilisomes, the large antenna protein complexes in heterocysts has long been debated. This study provides direct evidence of the involvement of these proteins in supporting nitrogenase activity in Anabaena 33047, a heterocystous cyanobacterium that has affinity for very high light intensities. This strain was previously known to be recalcitrant to genetic manipulation and hence despite its many appealing traits, remained largely unexplored. We developed a genetic modification system for this strain and generated a ΔnblA mutant that exhibited resistance to phycobilisome degradation upon nitrogen starvation. Physiological characterization of the strain indicated that PBS degradation is not essential for acclimation to nitrogen deficiency and retention of PBS is advantageous for nitrogenase function.

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Complete Genome Sequence of the Cyanobacterium Anabaena sp. 33047

Cited by 6 publications

References 16 publications

Thermophilic bacterial communities inhabiting the microbial mats of “indifferent” and chalybeate (iron‐rich) thermal springs: Diversity and biotechnological analysis

Thermophilic bacterial communities inhabiting the microbial mats of “indifferent” and chalybeate (iron‐rich) thermal springs: Diversity and biotechnological analysis

Antenna Modification Leads to Enhanced Nitrogenase Activity in a High Light-Tolerant Cyanobacterium

Antenna modification leads to enhanced nitrogenase activity in a high light tolerant cyanobacterium

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