Complete Genome Sequence of the Industrial Bacterium Ketogulonicigenium vulgare SKV

Nan, Jun; Ding, Ming-Zhu; Du, Yu-Zhang; Feng, Shuai; Gao, Feng; Yang, Yuan

doi:10.1128/genomea.01426-16

Cited by 6 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same recent studies on L. sericata bacterial content 11 , 35 revealed Proteus as the most abundant genus detected, being identified from the digestive tract, salivary glands and the adult stage 48 , 49 . However, the study of Gasz et al 46 revealed the dominant presence of Pseudomonas and Corynebacterium in all L. sericata organs, while Proteus was observed in low abundances.…”

Section: Discussionsupporting

confidence: 61%

Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica

Iancu

Angelescu

Paun

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The microbial diversity and quantitative dynamics during the insect’s development stages constitute recently developed putative tools in forensic and medical studies. Meanwhile, little is known on the role of insects in spreading foodborne pathogenic bacteria and on the impact of these pathogens on the overall insects and feeding substrate microbiome composition. Here, we provide the first characterization of the bacterial communities harbored in adult and immature stages of Lucilia sericata, one of the first colonizers of decomposed human remains, in the presence of the foodborne pathogen Salmonella enterica using 16S rRNA Illumina sequencing and qPCR. The pathogen transmission from the wild adults to the second generation was observed, with a 101.25× quantitative increase. The microbial patterns from both insect and liver samples were not influenced by the artificial introduction of this pathogenic foodborne bacteria, being dominated by Firmicutes and Proteobacteria. Overall, our results provided a first detailed overview of the insect and decomposed substrate microbiome in the presence of a human pathogen, advancing the knowledge on the role of microbes as postmortem interval estimators and the transmission of pathogenic bacteria.

show abstract

Section: Discussionsupporting

confidence: 61%

Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica

Iancu

Angelescu

Paun

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…vulgare strains (including K. vulgare SKV, SPU B805, Y25, Hbe602, and WSH-001) revealed that the strains SKV and SPU B805 lack 6-phosphofructokinase (EC 2.7.1.11, Pfk) of the EMP pathway, as well as the key enzymes 2-keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14, Eda) and 6-phosphogluconate dehydratase (EC 4.2.1.12, Edd) of the ED pathway. The EMP and ED pathways are therefore incomplete in these strains, and carbohydrate metabolism in the strains SKV and SPU B805 has been speculated to chiefly occur through the PPP pathway .…”

Section: Characteristics Of Key Strains Employed In the Classic Two-s...mentioning

confidence: 99%

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Liu,

Chen

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Vitamin C, also known as ascorbic acid, is an essential vitamin that cannot be synthesized by the human body and must be acquired through our diet. At present, the precursor of vitamin C, 2-keto-L-gulonic acid (2-KGA), is typically produced via a two-step fermentation process utilizing three bacterial strains. The second step of this traditional two-step fermentation method involves mixed-culture fermentation employing 2-KGA-producing bacteria (Ketogulonicigenium vulgare) along with associated bacteria. Because K. vulgare has defects in various metabolic pathways, associated bacteria are needed to provide key substances to promote K. vulgare growth and 2-KGA production. Unlike previous reviews where the main focus was the interaction between associated bacteria and K. vulgare, this Review presents the latest scientific research from the perspective of the metabolic pathways associated with 2-KGA production by K. vulgare and the mechanism underlying the interaction between K. vulgare and the associated bacteria. In addition, the dehydrogenases that are responsible for 2-KGA production, the 2-KGA synthesis pathway, strategies for simplifying 2-KGA production via a one-step fermentation route, and, finally, future prospects and research goals in vitamin C production are also presented.

show abstract

“…However, Ur Ba Nce et al [21] classified these bacteria as Ketogulonigenium vulgare after phenotype and genotype analysis in 2001. So far, whole-genome sequencing has been completed for K. vulgare Y25 [30], WSH-001 [31], Hbe602 [32], SKV [33], and SPU B805 [34].…”

Section: Metabolic Defects Of K Vulgare and The Effects Of Different ...mentioning

confidence: 99%

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Chen,

Liu,

Liu

et al. 2023

Fermentation

View full text Add to dashboard Cite

Vitamin C, a water-soluble vitamin with strong reducing power, cannot be synthesized by the human body and participates in a variety of important biochemical reactions. Vitamin C is widely used in the pharmaceutical, food, health care, beverage, cosmetics, and feed industries, with a huge market demand. The classical two-step fermentation method is the mainstream technology for vitamin C production. D-sorbitol is transformed into L-sorbose by Gluconobacter oxydans in the first step of fermentation; then, L-sorbose is transformed into 2-keto-L-gulonic acid (2-KGA) by a coculture system composed of Ketogulonicigenium vulgare and associated bacteria; and finally, 2-KGA is transformed into vitamin C through chemical transformation. The conversion of L-sorbose into 2-KGA in the second fermentation step is performed by K. vulgare. However, considering the slow growth and low 2-KGA production of K. vulgare when cultured alone, it is necessary to add an associated bacteria to stimulate K. vulgare growth and 2-KGA production. Although the mechanism by which the associated bacteria promote K. vulgare growth and 2-KGA production has extensively been studied, this remains a hot topic in related fields. Based on the latest achievements and research, this review summarizes the metabolic characteristics of K. vulgare and associated bacteria and elucidates the mechanism by which the associated bacteria promote the growth and 2-KGA production of K. vulgare.

show abstract

Complete Genome Sequence of the Industrial Bacterium Ketogulonicigenium vulgare SKV

Cited by 6 publications

References 15 publications

Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica

Microbiome pattern of Lucilia sericata (Meigen) (Diptera: Calliphoridae) and feeding substrate in the presence of the foodborne pathogen Salmonella enterica

Recent Advances in 2-Keto-l-gulonic Acid Production Using Mixed-Culture Fermentation and Future Prospects

Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review

Contact Info

Product

Resources

About