Complete genome sequence of the kirromycin producer Streptomyces collinus Tü 365 consisting of a linear chromosome and two linear plasmids

Rückert, Christian; Szczepanowski, Rafael; Albersmeier, Andreas; Goesmann, Alexander; Iftime, Dumitrita; Musiol, Ewa Maria; Blin, Kai; Wohlleben, Wolfgang; Pühler, Alfred; Kalinowski, Jörn; Weber, Tilmann

doi:10.1016/j.jbiotec.2013.10.004

Cited by 24 publications

(18 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Streptomyces bottropensis ATCC 25435 Produces bottromycin antibiotics. Streptomyces collinus Tu 365 (Rückert et al, 2013) Producer the elfamycin-family antibiotic kirromycin. Streptomyces exfoliatus DSMZ 41693 (Martínez et al, 2014) Degrades poly(3-hydroxyalkanoate).…”

Section: Species and Strain Motivation For Sequencingmentioning

confidence: 99%

Recently published Streptomyces genome sequences

Harrison

Studholme

2014

Microbial Biotechnology

View full text Add to dashboard Cite

Section: Species and Strain Motivation For Sequencingmentioning

confidence: 99%

Recently published Streptomyces genome sequences

Harrison

Studholme

2014

Microbial Biotechnology

View full text Add to dashboard Cite

“…Recently, the genome of S. collinus Tü 365 was sequenced using a combination of Sanger, 454 and illumina technology (Genbank ID: CP006259) [43]. S. collinus Tü 365 has a linear chromosome and contains two linear plasmids, SCO1 (85,041 bp, 68.3 % G+C), and SCO2 (19,314 bp, 69.9 % G+C).…”

Section: Genome Mining Of S Collinus Tü 365mentioning

confidence: 99%

“…A long terminal inverted repeat was found at both ends of the chromosome. Its size was determined to be 630 kb [43]. Genome sequencing indicated that both ends have identical sequences.…”

Section: Genome Mining Of S Collinus Tü 365mentioning

confidence: 99%

Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365

Iftime

Kulik

Härtner

et al. 2016

Journal of Industrial Microbiology and Biotechnology

Self Cite

View full text Add to dashboard Cite

Streptomycetes are prolific sources of novel biologically active secondary metabolites with pharmaceutical potential. S. collinus Tü 365 is a Streptomyces strain, isolated 1972 from Kouroussa (Guinea). It is best known as producer of the antibiotic kirromycin, an inhibitor of the protein biosynthesis interacting with elongation factor EF-Tu. Genome Mining revealed 32 gene clusters encoding the biosynthesis of diverse secondary metabolites in the genome of Streptomyces collinus Tü 365, indicating an enormous biosynthetic potential of this strain. The structural diversity of secondary metabolisms predicted for S. collinus Tü 365 includes PKS, NRPS, PKS-NRPS hybrids, a lanthipeptide, terpenes and siderophores. While some of these gene clusters were found to contain genes related to known secondary metabolites, which also could be detected in HPLC-MS analyses, most of the uncharacterized gene clusters are not expressed under standard laboratory conditions. With this study we aimed to characterize the genome information of S. collinus Tü 365 to make use of gene clusters, which previously have not been described for this strain. We were able to connect the gene clusters of a lanthipeptide, a carotenoid, five terpenoid compounds, an ectoine, a siderophore and a spore pigment-associated gene cluster to their respective biosynthesis products.

show abstract

“…The Actinomycete strain Streptomyces collinus Tü 365 (isolated in 1972 by Wolf and Zähner from Kouroussa, Guinea) is a producer of the antibiotic kirromycin (elfamycin family) . The genome of S. collinus Tü 365 was fully sequenced and annotated: 8.27 Mb encoding 7116 proteins . A genome mining approach led to the identification of a new representative of class IV lanthipeptides in S. collinus Tü 365.…”

Section: Introductionmentioning

confidence: 99%

“…[13] The genome of S. collinus Tü 365 was fully sequenced and annotated: 8.27 Mb encoding 7116 proteins. [14] Ag enome mininga pproach [15] led to the identification of an ew representative of class IV lanthipeptides in S. collinus Tü 365. Here we report the identification, cloning, and analysis of this lanthipeptide,w hich we name streptocollin.…”

Section: Introductionmentioning

confidence: 99%

Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365

et al. 2015

Self Cite

View full text Add to dashboard Cite

Lanthipeptides are ribosomally synthesized and post‐translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5‐ and 5.5‐fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster.

show abstract

Complete genome sequence of the kirromycin producer Streptomyces collinus Tü 365 consisting of a linear chromosome and two linear plasmids

Cited by 24 publications

References 12 publications

Recently published Streptomyces genome sequences

Recently published Streptomyces genome sequences

Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365

Streptocollin, a Type IV Lanthipeptide Produced by Streptomyces collinus Tü 365

Contact Info

Product

Resources

About