Complete Genome Sequence of “ Candidatus Viadribacter manganicus” Isolated from a German Floodplain Area

Abstract

Iron- and manganese-depositing bacteria occur in many soils and all water systems, and their biogenic depositions of ochre in technical systems may cause severe clogging problems and monetary losses. “Candidatus Viadribacter manganicus” is a small coccoid, iron- and manganese-depositing bacterium isolated from the Lower Oder Valley National Park, Germany.

Evaluation of V2 18S rDNA barcode marker and assessment of sample collection and DNA extraction methods for metabarcoding of autotrophic euglenids

“…Water DNA Kit (Omega Bio‐tek; WAT(O)). The selected methods are commonly used in metabarcoding surveys (Kaden and Krolla‐Sidenstein, 2015 ; Braun and Szewzyk, 2016 ; Wang et al ., 2018 ; Gołębiewski and Tretyn, 2019 ; Jeunen et al ., 2019 ). All isolation steps were performed according to the manufacturer's instructions.…”

“…presence of amplification inhibitors, such as humic acids), (iv) cost of reagents, (v) preparation complexity and time required to process a single sample. (Kaden and Krolla-Sidenstein, 2015;Braun and Szewzyk, 2016;Wang et al, 2018;Gołębiewski and Tretyn, 2019;Jeunen et al, 2019). All isolation steps were performed according to the manufacturer's instructions.…”

Draft Genome Sequence of Actinobacterial Strain Kineosporia sp. R_H_3, a Neutrophilic Iron-Depositing Bacterium

“…Extraction of genomic DNA was done as previously described ( 7 ), and a paired-end library was prepared according to the Illumina Nextera XT DNA library prep kit protocol. Genome sequencing was performed on an Illumina NextSeq 500 sequencer using NextSeq mid output kit v2 (300-cycle) chemistry, which generated 19,900,890 raw reads.…”

Draft Genome Sequence of Strain R_RK_3, an Iron-Depositing Isolate of the Genus Rhodomicrobium , Isolated from a Dewatering Well of an Opencast Mine

“…Extraction of genomic DNA was done as previously described ( 12 ). The paired-end library was prepared by following the Illumina Nextera XT DNA library prep kit protocol.…”