Complete Genome Sequences of Mannanase-Producing
            <i>Bacillus</i>
            and
            <i>Niallia</i>
            Strains Isolated from the Intestine of the Black Tiger Shrimp (Penaeus monodon)

Sathitkowitchai, Witida; Wongsurawat, Thidathip; Jenjaroenpun, Piroon; Angthong, Pacharaporn; Nuanpirom, Jiratchaya; Sathapondecha, Ponsit; Rungrassamee, Wanilada

doi:10.1128/mra.00112-22

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…We previously determined complete full genome sequences of our bacterial candidates, Man26 and Man122, in which they showed similarity to Niallia sp. and Bacillus sp., respectively [ 43 ]. The morphological characteristics of the two isolates showed that both Man26 and Man122 were Gram positive with a rod shape ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Mannanase-Producing Bacteria for Potential Synbiotic Application in Shrimp Farming

Sathitkowitchai

Sathapondecha

Angthong

et al. 2022

Animals

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Prebiotics such as mannan-oligosaccharides (MOS) are a promising approach to improve performance and disease resistance in shrimp. To improve prebiotic utilization, we investigated the potential probiotics and their feasibility of synbiotic use in vitro. Two bacterial isolates, Man26 and Man122, were isolated from shrimp intestines and screened for mannanase, the enzyme for mannan digestion. The crude mannanase from both isolates showed optimal activities at pH 8 with optimum temperatures at 60 °C and 50 °C, respectively. The enzymes remained stable at pH 8–10 for 3 h (>70% relative activity). The thermostability range of Man26 was 20–40 °C for 20 min (>50%), while that of Man122 was 20–60 °C for 30 min (>50%). The Vmax of Man122 against locust bean gum substrate was 41.15 ± 12.33 U·mg−1, six times higher than that of Man26. The Km of Man26 and Man122 were 18.92 ± 4.36 mg·mL−1 and 34.53 ± 14.46 mg·mL−1, respectively. With the addition of crude enzymes, reducing sugars of copra meal, palm kernel cake, and soybean meal were significantly increased (p < 0.05), as well as protein release. The results suggest that Man26 and Man122 could potentially be used in animal feeds and synbiotically with copra meal to improve absorption and utilization of feedstuffs.

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Section: Resultsmentioning

confidence: 99%