Complete Genome Sequences of Nine Phages Capable of Infecting Paenibacillus larvae, the Causative Agent of American Foulbrood Disease in Honeybees

Tsourkas, Philippos K.; Yost, Diane G.; Krohn, Andrew; LeBlanc, Lucy; Zhang, Anna; Stamereilers, Casey; Amy, Penny S.

doi:10.1128/genomea.01120-15

Cited by 19 publications

(20 citation statements)

References 7 publications

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“…92% similarity with the TLS of vB_BceS-IEBH (YP_002154374.1) and 80% with the TLS of A118 (NP_463463.1) [ 20 , 23 ]. The TLS of PfEFR-4 and 11143 revealed 77% and 46% similarity, respectively, with that of Paenibacillus phage Harrison (YP_009193815.1), which has site-specific packaging with “cohesive ends with 3’ overhangs” [ 24 ]. The TLS of Tp250 shared 85% similarity with that of PBC1 (YP_006383455.1), which carries terminally redundant and partially permuted genomes [ 25 ], thus Tp250 might also have a headful packaging mechanism.…”

Section: Resultsmentioning

confidence: 99%

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

et al. 2017

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Cereulide-producing Bacillus cereus isolates can cause serious emetic (vomiting) syndrome and even acute lethality. As mobile genetic elements, the exploration of prophages derived from emetic B. cereus isolates will help in our understanding of the genetic diversity and evolution of these pathogens. In this study, five temperate phages derived from cereulide-producing B. cereus strains were induced, with four of them undergoing genomic sequencing. Sequencing revealed that they all belong to the Siphoviridae family, but presented in different forms in their hosts. PfNC7401 and PfIS075 have typical icosahedral heads, probably existing alone as phagemids in the host with self-replicating capability in the lysogenic state. PfEFR-4, PfEFR-5, and PfATCC7953 have elongated heads, with the genomes of the former two identified as linear dsDNA, which could be integrated into the host genome during the lysogenic state. Genomic comparison of the four phages with others also derived from emetic B. cereus isolates showed similar genome structures and core genes, thus displaying host spectrum specificity. In addition, phylogenic analysis based on the complete genome and conserved tail fiber proteins of 36 Bacillus species-derived phages confirmed that the phages derived from emetic B. cereus strains were highly similar. Furthermore, one endolysin LysPfEFR-4 was cloned and showed lytic activity against all tested emetic B. cereus strains and cross-lytic activity against some other pathogenic bacteria, implying a potential to control bacterial contamination in the food supply.

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Section: Resultsmentioning

confidence: 99%

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

et al. 2017

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“…The results demonstrated that the majority of these isolates were genetically unique. 42 GenBank accession numbers for 9 of the phages are as follows: KT361649, KT361650, KT361651, KT361652, KT361653, KT361654, KT361655, KT361656, KT361657.…”

Section: Resultsmentioning

confidence: 99%

Experimental bacteriophage treatment of honeybees (Apis mellifera) infected withPaenibacillus larvae, the causative agent of American Foulbrood Disease

2016

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American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium Paenibacillus larvae. One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse P. larvae. Therefore, bacteriophages specific for P. larvae were isolated to determine their efficacy in lysing P. larvae cells. Samples from soil, beehive materials, cosmetics, and lysogenized P. larvae strains were screened; of 157 total samples, 28 were positive for at least one P. larvae bacteriophage, with a total of 30. Newly isolated bacteriophages were tested for the ability to lyse each of 11 P. larvae strains. Electron microscopy demonstrated that the phage isolates were from the family Siphoviridae. Seven phages with the broadest host ranges were combined into a cocktail for use in experimental treatments of infected bee larvae; both prophylactic and post-infection treatments were conducted. Results indicated that although both pre- and post-treatments were effective, prophylactic administration of the phages increased the survival of larvae more than post-treatment experiments. These preliminary experiments demonstrate the likelihood that phage therapy could be an effective method to control AFB.

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“…Assembly was carried out using Geneious 7.1 (Biomatters, Auckland, NZ). 38 Details of the assembly process are given in 26 .…”

Section: Methodsmentioning

confidence: 99%

“… 40 We also used GeneMark.hmm ( exon.gatech.edu ). 41 Details of the annotation procedure are given in 26 .…”

Section: Methodsmentioning

confidence: 99%

Comparative genomics of 9 novelPaenibacillus larvaebacteriophages

et al. 2016

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American Foulbrood Disease, caused by the bacterium Paenibacillus larvae, is one of the most destructive diseases of the honeybee, Apis mellifera. Our group recently published the sequences of 9 new phages with the ability to infect and lyse P. larvae. Here, we characterize the genomes of these P. larvae phages, compare them to each other and to other sequenced P. larvae phages, and putatively identify protein function. The phage genomes are 38–45 kb in size and contain 68–86 genes, most of which appear to be unique to P. larvae phages. We classify P. larvae phages into 2 main clusters and one singleton based on nucleotide sequence identity. Three of the new phages show sequence similarity to other sequenced P. larvae phages, while the remaining 6 do not. We identified functions for roughly half of the P. larvae phage proteins, including structural, assembly, host lysis, DNA replication/metabolism, regulatory, and host-related functions. Structural and assembly proteins are highly conserved among our phages and are located at the start of the genome. DNA replication/metabolism, regulatory, and host-related proteins are located in the middle and end of the genome, and are not conserved, with many of these genes found in some of our phages but not others. All nine phages code for a conserved N-acetylmuramoyl-L-alanine amidase. Comparative analysis showed the phages use the “cohesive ends with 3′ overhang” DNA packaging strategy. This work is the first in-depth study of P. larvae phage genomics, and serves as a marker for future work in this area.

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Complete Genome Sequences of Nine Phages Capable of Infecting Paenibacillus larvae, the Causative Agent of American Foulbrood Disease in Honeybees

Cited by 19 publications

References 7 publications

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

Identification and genomic comparison of temperate bacteriophages derived from emetic Bacillus cereus

Experimental bacteriophage treatment of honeybees (Apis mellifera) infected withPaenibacillus larvae, the causative agent of American Foulbrood Disease

Comparative genomics of 9 novelPaenibacillus larvaebacteriophages

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