Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel

Galinier, Richard; Beurden, Steven van; Amilhat, Elsa; Castric, J.; Schoehn, Guy; Verneau, Olivier; Fazio, G.; Allienne, Jean‐François; Engelsma, Marc; Sasal, Pierre; Faliex, Elisabeth

doi:10.1016/j.virusres.2012.02.020

Cited by 26 publications

(29 citation statements)

References 71 publications

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“…Phylogenetic analysis also revealed SCRV as the closest relative of MoARV, and they were further grouped with the recently characterized EVEX. More over, these viruses formed a genetic cluster distinct from the one represented by SVCV and PFRV, supporting the potential existence of a new fish vesiculovirus clade as previously re ported (Galinier et al 2012, Stone et al 2013. Rice field eels belong to the Synbranchiformes, whose members are taxonomically closely related to those of the Perciformes (Johnson & Patterson 1993).…”

Section: Discussionsupporting

confidence: 59%

“…EVEX was considered a member of the species Anguillid rhabdovirus (Stone et al 2013). After EVEX was isolated in a shipment of European elvers Anguilla anguilla from France to Tokyo, another rhabdo virus appearing to be serologically similar to EVEX was reported in Japan (Kobayashi & Miyazaki 1996, Galinier et al 2012). This information suggests that rhabdoviruses also have a noticeable impact on percid farming in Asia and may evolve to infect taxonomically related host species.…”

Section: Discussionmentioning

confidence: 99%

“…For the MoARV N and G proteins, the highest similarities (35.4−97.3%) are to members of the genus Vesi cu lovirus. The family Rhabdoviridae currently includes 6 genera: Vesiculovirus, Lyssavirus, Ephemerovirus, Novi rhabdovirus, Cytorhabdovirus, and Nucleo rhabdovirus (Tordo et al 2005), and to date all reported rhabdoviruses infecting fish have been classified in the genera Vesiculovirus or Novirhabdovirus (Galinier et al 2012). Phylogenetic analysis also revealed SCRV as the closest relative of MoARV, and they were further grouped with the recently characterized EVEX.…”

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confidence: 99%

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Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus

Ou¹,

Zhu²,

Chen³

et al. 2013

Dis. Aquat. Org.

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We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 × 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease. KEY WORDS: Fish viral disease · Rice field eel · Monopterus albus rhabdovirus · MoARV · Histopathology · Electron microscopy · Phylogenetic analysisResale or republication not permitted without written consent of the publisher Dis Aquat Org 106: 197-206, 2013 (SMRV), have been identified and isolated from diseased mandarin fish Siniperca chuatsi (Zhang & Li 1999), Chinese sucker Myxocyprinus asiaticus (Zhang et al. 2000), and turbot Scophthalmus maximus (Zhang et al. 2007), respectively. These viruses have been reported to be associated with high morbidity and mortality in a number of aquaculture species and have tentatively been assigned to the family Rhabdoviridae. Rhabdoviruses have a serious socio-economic impact on aquaculture, particularly infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), and spring viremia of carp virus (SVCV), which are all responsible for major disease epizootics in aquaculture and generally result in an acute hemorrhagic syndrome (Betts et al. 2003, Crane & Hyatt 2011.In this study, we collected diseased fish showing typical signs and attempted to isolate a virus. Based on a series of observations and analysis, a rhabdovirus was identified as the causative pathogen responsible for the systemic hemorrhagic disease. This is the first disease caused by a virus to be recorded in rice field eels. MATERIALS AND METHODS Sample coll...

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus

Ou¹,

Zhu²,

Chen³

et al. 2013

Dis. Aquat. Org.

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“…Synonymous names used for EVEX/EVA were rhabdovirus anguilla (Hill et al 1980, Shchelkunov et al 1989) and rhabdoviral dermatitis of Japanese eel (Kobayashi & Miyazaki 1996). EVEX/EVA belongs to the group of fish vesiculovirus-like isolates, family Rhabdoviridae, order Mononegavirales (Galinier et al 2012). …”

mentioning

confidence: 99%

Viral diseases of wild and farmed European eel Anguilla anguilla with particular reference to the Netherlands

Beurden

Engelsma²,

Roozenburg³

et al. 2012

Dis. Aquat. Org.

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“…For EVEX Galinier et al . (), isolate CVI NL‐108778 was used. For EVE, the genetically closely related Infectious pancreatic necrosis virus (IPNV) strain Ab (Blake et al .…”

Section: Methodsmentioning

confidence: 99%

Development and validation of a real‐time PCR assay for the detection of anguillid herpesvirus 1

Beurden

Voorbergen-Laarman

Roozenburg

et al. 2015

Journal of Fish Diseases

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Anguillid herpesvirus 1 (AngHV1) causes a haemorrhagic disease with increased mortality in wild and farmed European eel, Anguilla anguilla (L.) and Japanese eel Anguilla japonica, Temminck & Schlegel). Detection of AngHV1 is currently based on virus isolation in cell culture, antibody-based typing assays or conventional PCR. We developed, optimized and concisely validated a diagnostic TaqMan probe based real-time PCR assay for the detection of AngHV1. The primers and probe target AngHV1 open reading frame 57, encoding the capsid protease and scaffold protein. Compared to conventional PCR, the developed real-time PCR is faster, less labour-intensive and has a reduced risk of cross-contamination. The real-time PCR assay was shown to be analytically sensitive and specific and has a high repeatability, efficiency and r 2 -value. The diagnostic performance of the assay was determined by testing 10% w/v organ suspensions and virus cultures from wild and farmed European eels from the Netherlands by conventional and realtime PCR. The developed real-time PCR assay is a useful tool for the rapid and sensitive detection of AngHV1 in 10% w/v organ suspensions from wild and farmed European eels.

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Complete genomic sequence and taxonomic position of eel virus European X (EVEX), a rhabdovirus of European eel

Cited by 26 publications

References 71 publications

Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus

Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus

Viral diseases of wild and farmed European eel Anguilla anguilla with particular reference to the Netherlands

Development and validation of a real‐time PCR assay for the detection of anguillid herpesvirus 1

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