Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China

Abstract

A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. The first segment was 2088 bp long and contained a single ORF (ORF1) encoding the RNA-dependent RNA polymerase (RdRP) (72.26 kDa). The second segment was 1634 bp long and also contained a single ORF (ORF2) encoding a hypothetical protein of 37.472 kDa. We named this novel mycovirus “Trichoderma harzianum bipartite mycovirus 1” (ThBMV1). Phylogenetic analysis showed that ThBMV1 clusters with other unclassified dsRNA mycoviruses.Electronic supplementary materialThe online version of this article (10.1007/s00705-019-04145-9) contains supplementary material, which is available to authorized users.

The Newly Identified Trichoderma harzianum Partitivirus (ThPV2) Does Not Diminish Spore Production and Biocontrol Activity of Its Host

“…In this context, it is worth noting that the genomes of only eight mycoviruses, including ThPV2, have been sequenced worldwide to date. The other viruses are two mycoviruses from Trichoderma atroviride (Trichoderma atroviride mycovirus 1 [ 15 , 16 ] and Trichoderma atroviride partitivirus 1 [ 17 ]), four mycoviruses from T. harzianum (Trichoderma harzianum bipartite mycovirus 1 (ThMBV1) [ 19 ], Trichoderma harzianum mycovirus 1 (ThMV1) [ 20 ], Trichoderma harzianum hypovirus 1 (ThHV1) [ 22 ], and Trichoderma harzianum partitivirus 1 (ThPV1) [ 17 ]), and a single virus from Trichoderma asperellum (Trichoderma asperellum dsRNA virus 1 [ 18 ]). Of these, one mycovirus belongs to the Fusagraviridae , two belong to the Partitiviridae , one is a Betahypovirus , and three mycoviruses remain unclassified so far.…”

“…Complete genome sequencing of dsRNA viruses from T. atroviride NFCF394 and T. harzianum NFCF319 identified Trichoderma atroviride partitivirus 1 (TaPV1) and Trichoderma harzianum partitivirus 1 (ThPV1) as novel mycoviruses of the family of Partitiviridae [ 16 , 17 ]. More recently, five novel mycoviruses, i.e., Trichoderma asperellum dsRNA virus 1 (TaRV1) [ 18 ], Trichoderma harzianum mycovirus 1 (ThMBV1) [ 19 ], Trichoderma harzianum mycovirus 1 (ThMV1) [ 20 ], Trichoderma harzianum hypovirus 1 (ThHV1) [ 21 ], and the new (+)ss RNA hypovirus Trichoderma harzianum hypovirus 2 (ThHV2) [ 22 ], were identified.…”

Viruses of endophytic and pathogenic forest fungi

“…Viruses have been detected in all forms of fungi, from endophytes (e.g., Trichoderma harzianum) to obligate parasites (e.g., Puccinia striiformis) [30,31]. Bao and Roossinck [32] provided an overview of the putative viruses of 52 distinct endophytes, revealing that almost all endophyte classes are assumed to have mycoviruses living in them (for a more detailed description refer to [32]).…”

Molecular characterization of a novel victorivirus isolated from the phytopathogenic fungus Phaeobotryon rhois

“…Mycoviruses can infect almost all types of fungi, including fungi, yeasts, and various oomycetes, such as phytopathogenic fungi, biocontrol fungi, and endophytic fungi [1][2][3]. Mycoviruses can have different genomic structures, including double-stranded (dsRNA), positive single-stranded RNA (+ ssRNA), negative single-stranded RNA (-ssRNA), and single-stranded DNA (ssDNA) [1,4].…”