Complete Nucleotide Sequence of a Cryptic Plasmid from the Ruminal Bacterium Selenomonas ruminantium HD4 and Identification of Two Predicted Open Reading Frames

Al-Khaldi, Sufian F.; Evans, J. D.; Martin, Scott A.

doi:10.1006/plas.1999.1405

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…Plasmids isolated from rumen bacteria have been characterized as having replication and mobilization functions, and some contain accessory genes (25)(26)(27)(28). As most reticulorumen microorganisms cannot be cultured, the ability to study their resident plasmids is limited by the constraints of traditional microbiology methods.…”

mentioning

confidence: 99%

Insights into the bovine rumen plasmidome

Kav

Sasson

Jami

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

137

115

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Plasmids are self-replicating genetic elements capable of mobilization between different hosts. Plasmids often serve as mediators of lateral gene transfer, a process considered to be a strong and sculpting evolutionary force in microbial environments. Our aim was to characterize the overall plasmid population in the environment of the bovine rumen, which houses a complex and dense microbiota that holds enormous significance for humans. We developed a procedure for the isolation of total rumen plasmid DNA, termed rumen plasmidome, and subjected it to deep sequencing using the Illumina paired-end protocol and analysis using public and custom-made bioinformatics tools. A large number of plasmidome contigs aligned with plasmids of rumen bacteria isolated from different locations and at various time points, suggesting that not only the bacterial taxa, but also their plasmids, are defined by the ecological niche. The bacterial phylum distribution of the plasmidome was different from that of the rumen bacterial taxa. Nevertheless, both shared a dominance of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. Evidently, the rumen plasmidome is of a highly mosaic nature that can cross phyla. Interestingly, when we compared the functional profile of the rumen plasmidome to two plasmid databases and two recently published rumen metagenomes, it became apparent that the rumen plasmidome codes for functions, which are enriched in the rumen ecological niche and could confer advantages to their hosts, suggesting that the functional profiles of mobile genetic elements are associated with their environment, as has been previously implied for viruses.

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mentioning

confidence: 99%

Insights into the bovine rumen plasmidome

Kav

Sasson

Jami

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

137

115

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Selenomonas

Shouche¹,

Dighe²,

Dhotre³

et al. 2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Se.le.no.mo'nas. Gr. n. selene the moon; Gr. n. monas a unit, monad; N.L. fem. n. Selenomonas moon (‐shaped) monad. Firmicutes / “Clostridia” / Clostridiales / Veillonellaceae / Selenomonas Curved to helical rods , usually 0.9–1.1 × 3.0–6.0 µm. The ends are usually tapered and rounded to give short kidney‐ to crescent‐shaped or vibrioid cells . Long cells and chains of cells are often helical. Capsules are not formed. Resting stages are not known. Gram‐stain‐negative. Motile with active tumbling ; flagella ( up to 16 ) are arranged linearly as a tuft near the center of the concave side in the area of cell fission . Strictly anaerobic . Optimum temperature 35–40°C; maximum 45°C; minimum 20–30°C. Chemoorganotrophic, having a fermentative type of metabolism . Carbohydrates and lactate can serve as fermentable substrates . Fermentation of glucose chiefly yields acetate , propionate , CO 2 , and / or lactate . Small amounts of H 2 and succinate may be produced. Catalase negative. DNA G + C content ( mol %): 40–61. Type species : Selenomonas sputigena (Flügge 1886) Boskamp 1922, 70 AL ( Spirillum sputigenum Flügge 1886, 387).

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Plasmids ofSelenomonas ruminantium and development of host-vector system

et al. 2001

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A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50% tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25% of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coli vector pBluescriptSK+ and partially characterized. The plasmid pSRD181 is 1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data. Strain S. ruminantium 28 was successfully transformed with pJW1 derived plasmid pJ1B1 using ampicillin resistance gene as marker. This is the first report on transformation of selenomonads with foreign DNA.

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Complete Nucleotide Sequence of a Cryptic Plasmid from the Ruminal Bacterium Selenomonas ruminantium HD4 and Identification of Two Predicted Open Reading Frames

Cited by 5 publications

References 22 publications

Insights into the bovine rumen plasmidome

Insights into the bovine rumen plasmidome

Selenomonas

Plasmids ofSelenomonas ruminantium and development of host-vector system

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