Complete nucleotide sequence of a new potexvirus, ‘Cnidium virus X’, isolated from Cnidium officinale in Japan

Honma, Hiroshi; Tsushima, Daiki; Kawakami, Hiroko; Fujihara, Noború; Tsusaka, Takeshi; Kawashimo, Midori; Nishimura, Tomohiro; Fuji, Shin Ichi

doi:10.1007/s00705-019-04261-6

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…This capability is particularly crucial in the context of viral diseases, where early detection plays a pivotal role in implementing effective control strategies. In previous studies, researchers have investigated viruses infecting chunkugn plants [ 9 , 28 ]. However, comprehensive studies on the chunkugn virome have been limited [ 3 , 4 , 9 , 29 ], and there were no reports of DNA viruses.…”

Section: Discussionmentioning

confidence: 99%

“…For example, monocultures, irrigation systems, extended growing seasons, the transport of seedlings, changes in land use, and the application of artificial soil amendments can alter ecological dynamics and favor the emergence and spread of specific plant viruses [ 20 , 27 ]. Several economically important viral diseases affect chunkung [ 28 , 29 ], and the emergence of novel viruses is increasingly being detected because of advances in high-throughput sequencing (HTS) technologies.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Belete,

Kim,

Gudeta

et al. 2024

Virol J

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Background Viruses have notable effects on agroecosystems, wherein they can adversely affect plant health and cause problems (e.g., increased biosecurity risks and economic losses). However, our knowledge of their diversity and interactions with specific host plants in ecosystems remains limited. To enhance our understanding of the roles that viruses play in agroecosystems, comprehensive analyses of the viromes of a wide range of plants are essential. High-throughput sequencing (HTS) techniques are useful for conducting impartial and unbiased investigations of plant viromes, ultimately forming a basis for generating further biological and ecological insights. This study was conducted to thoroughly characterize the viral community dynamics in individual plants. Results An HTS-based virome analysis in conjunction with proximity sampling and a tripartite network analysis were performed to investigate the viral diversity in chunkung (Cnidium officinale) plants. We identified 61 distinct chunkung plant-associated viruses (27 DNA and 34 RNA viruses) from 21 known genera and 6 unclassified genera in 14 known viral families. Notably, 12 persistent viruses (7 DNA and 5 RNA viruses) were exclusive to dwarfed chunkung plants. The detection of viruses from the families Partitiviridae, Picobirnaviridae, and Spinareoviridae only in the dwarfed plants suggested that they may contribute to the observed dwarfism. The co-infection of chunkung by multiple viruses is indicative of a dynamic and interactive viral ecosystem with significant sequence variability and evidence of recombination. Conclusions We revealed the viral community involved in chunkung. Our findings suggest that chunkung serves as a significant reservoir for a variety of plant viruses. Moreover, the co-infection rate of individual plants was unexpectedly high. Future research will need to elucidate the mechanisms enabling several dozen viruses to co-exist in chunkung. Nevertheless, the important insights into the chunkung virome generated in this study may be relevant to developing effective plant viral disease management and control strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Belete,

Kim,

Gudeta

et al. 2024

Virol J

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“…Basic residues are in red and hydrophobic residues are in green. Viruses were selected based on the RdRp phylogeny of potexviruses [40]. OpVX, opuntia virus X; CsCMV, cassava common mosaic virus; PapMV, papaya mosaic virus; ClYMV, clover yellow mosaic virus; BaMV, bamboo mosaic virus; PlAMV, plantago asiatica mosaic virus; CsVX, cactus virus X; PepMV, pepino mosaic virus; AVX, actinidia virus X.…”

Section: Prediction Of Membrane-immersed Amino Acidsmentioning

confidence: 99%

The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization

Jiang

Luan

Chai

et al. 2022

Viruses

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Positive-sense single-stranded RNA viruses replicate in virus-induced membranous organelles for maximum efficiency and immune escaping. The replication of potato virus X (PVX) takes place on the endoplasmic reticulum (ER); however, how PVX-encoded RNA-dependent RNA polymerase (RdRp) is associated with the ER is still unknown. A proline-kinked amphipathic α-helix was recently found in the MET domain of RdRp. In this study, we further illustrate that the first α-helix of the MET domain is also required for ER association. Moreover, we found that the MET domain forms multimers on ER and the first α-helix is essential for multimerization. These results suggest that the RdRp of PVX adopts more than one hydrophobic motif for membrane association and for multimerization.

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Complete genome sequence of cnidium closterovirus 1, a novel member of the genus Closterovirus infecting Cnidium officinale

et al. 2022

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Complete nucleotide sequence of a new potexvirus, ‘Cnidium virus X’, isolated from Cnidium officinale in Japan

Cited by 7 publications

References 20 publications

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

Deciphering the virome of Chunkung (Cnidium officinale) showing dwarfism-like symptoms via a high-throughput sequencing analysis

The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization

Complete genome sequence of cnidium closterovirus 1, a novel member of the genus Closterovirus infecting Cnidium officinale

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