Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements

Dunn, John J.; Studier, F. William; Gottesman, M.

doi:10.1016/s0022-2836(83)80282-4

Cited by 1,308 publications

(884 citation statements)

References 84 publications

Supporting

Mentioning

864

Contrasting

Unclassified

Order By: Relevance

“…The phage T7 early transcriptional terminator (t E ) was isolated by polymerase chain reaction (PCR) amplification of T7 basepairs 7550-7607 (Dunn and Studier, 1983), such that convenient restriction sites were added to the termini of the PCR product. This DNA fragment was then digested with Hin dIII and BamHI and inserted into the polycloning site of pUC19 to yield pRS3064.…”

Section: Methodsmentioning

confidence: 99%

“…3Ј region of 'mini-rnc ' genes. Shown are the nucleotide sequence and putative secondary structure of the rho-independent early transcriptional terminator (t E ) from bacteriophage T7 (Dunn and Studier, 1983), fused at various positions to the rnc gene (numbered from the start of transcription) just downstream of an engineered, in frame stop codon (enlarged) and sequences added during construction. The resulting 'mini-rnc ' genes (see Fig.…”

Section: Increased Rnc Gene Translation Abrogates Rnase III Regulationmentioning

confidence: 99%

See 1 more Smart Citation

Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation

Matsunaga

Simons

1997

Molecular Microbiology

View full text Add to dashboard Cite

SummaryControl of mRNA stability is an established means of regulating gene expression. However, the detailed mechanisms by which such control is achieved are only now emerging. In particular, there remains a question about the involvement of translation. Escherichia coli ribonuclease III (RNase III) negatively autoregulates expression of its own gene (rnc) approximately 10-fold, by cleaving the untranslated leader and initiating approximately 10-fold more rapid decay of the rnc mRNA, after which RNase III plays no further role. Here, we define the mechanism of this control further. Mutations that increase rnc gene translation abolish autoregulation by increasing the stability of the RNase III-cleaved transcript RNA approximately 10-fold, with no effect on the uncleaved species. Mutations that decrease translation destabilize the rnc mRNA in the presence or absence of RNase III. In so doing, they reveal a pathway of rnc transcript decay distinct from the RNase III-dependent pathway. Stability of a 'minirnc' transcript containing the rnc leader and only the first two codons of the rnc gene is unaffected by decreased translation, presumably because sequences required for this pathway were removed. Importantly, this mini-rnc transcript is regulated normally by RNase III. Moreover, rnc transcripts synthesized in vitro do not decay in cell-free extracts lacking ribosomes, unless they are first cleaved by RNase III. These two results show that RNase III cleavage can initiate rnc transcript decay independently of rnc gene translation, unambiguously establishing that control of mRNA decay need not involve changes in translation. How rnc gene translation is optimized for efficient autoregulation will also be discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Increased Rnc Gene Translation Abrogates Rnase III Regulationmentioning

confidence: 99%

Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation

Matsunaga

Simons

1997

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…Purified products were cloned using the pGem ® -T Easy cloning kit (Promega, Madison, USA) after which cloned inserts were amplified directly from colonies using the vector-specific primers T7 and SP6 (Butler and Chamberlin 1982;Dunn et al 1983). The latter PCRs utilized the same PCR reaction and cycling conditions as before, with the only exception that 30 amplification cycles instead of 35 were used.…”

Section: Pcr Cloning and Sequencing Of The Mating Type Genesmentioning

confidence: 99%

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

View full text Add to dashboard Cite

In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses on agar plates, the heterothallic nature of the wood-inhabiting fungus Ophiostoma quercus was confirmed and mating types were assigned to 10 isolates. Primers were subsequently designed to target both the mating type 1 (MAT1-1) and 2 (MAT1-2) idiomorphs in these isolates. The results showed that all isolates contained sequence fragments representing both idiomorphs. This was unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs. An atypical mating system such as the one described in this study has not previously been encountered in any other Ascomycota mating locus described to date.

show abstract

“…Treatment of various concentrations (25 to 100 μg/ml) of T7 DNA (37.9 kbp; molecular weight 25 × 10 6 daltons; 48% GC [19]) with a range of concentrations of 90CE up to 200 μM gave a 90CE concentration dependent increase in the level of cross-linking ( Figure 2A) that was independent of the concentration of DNA ( Figure 2B). The molar yield of cross-links produced under these conditions was readily calculated from the fractional cross-linking value (i.e., the fraction of DNA molecules containing one or more cross-links per molecule) by assuming a Poisson distribution ( Figure 2C, 2D).…”

Section: Relationship Between Dna Cross-linking and Concentrations Ofmentioning

confidence: 99%

Lethality to leukemia cell lines of DNA interstrand cross-links generated by Cloretazine derived alkylating species

Penketh¹,

Baumann²,

Ishiguro³

et al. 2008

Leukemia Research

View full text Add to dashboard Cite

Cloretazine [1, 2-bis(methylsulfonyl)-1-(2-chloroethyl)-2-[(methylamino)carbonyl]-hydrazine; VNP40101M; 101M] is a relatively new prodrug with activity in elderly acute myelogenous leukemia patients. Its therapeutic action is due largely to the production of 1-(3-cytosinyl),2-(1-guanyl)ethane cross-links (G-C ethane cross-links) in DNA. The number of cross-links produced in three experimental leukemia lines (L1210, U937 and HL-60) were fewer than 10 per genome at their respective LC 50 concentrations. Only 1 in approximately 20,000 90CE molecules produce a crosslink in the AGT (O 6 -alkylguanine-DNA alkyltransferase) negative L1210 and U937 cell lines and 1 in 400,000 in the AGT positive HL-60 cell line.

show abstract

Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements

Cited by 1,308 publications

References 84 publications

Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation

Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Lethality to leukemia cell lines of DNA interstrand cross-links generated by Cloretazine derived alkylating species

Contact Info

Product

Resources

About