Complete nucleotide sequences of genome segments 1 and 3 of Rosellinia anti-rot virus in the family Reoviridae

Wei, Chuan Zhao; Osaki, Hideki; Iwanami, Toru; Matsumoto, Naoyuki; Ohtsu, Yoshihiro

doi:10.1007/s00705-003-0259-6

Cited by 39 publications

(14 citation statements)

References 15 publications

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“…Genus Mycoreovirus was then created to accommodate three species, Mycoreovirus 1 to Mycoreovirus 3 (including well-characterized representative isolates MyRV1 to MyRV3, respectively). MyRV1 and -2 were isolated from Cryphonectria parasitica, and MyRV3 from Rosellinia necatrix (Hillman and Suzuki, 2004;Wei et al, 2004). All mycoreoviruses confer hypovirulence to their respective natural hosts.…”

Section: Reoviridae (Dsrna)mentioning

confidence: 99%

50-plus years of fungal viruses

et al. 2015

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Section: Reoviridae (Dsrna)mentioning

confidence: 99%

50-plus years of fungal viruses

et al. 2015

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“…6Q). It has been predicted that sense-strandspecific inverted repeat and panhandle structures exist between the 5= and 3= termini of genome segments of RnQV1, RnVV1, and RnMyRV3 (29,34,40,61); therefore, these secondary structures might be diced. In contrast, we found that vsRNA peaks were densely distributed at the 5= region of both RnPV1-S1 and RnPV1-S2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Differential Inductions of RNA Silencing among Encapsidated Double-Stranded RNA Mycoviruses in the White Root Rot Fungus Rosellinia necatrix

Yaegashi

Shimizu

Ito

et al. 2016

J Virol

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RNA silencing acts as a defense mechanism against virus infection in a wide variety of organisms. Here, we investigated inductions of RNA silencing against encapsidated double-stranded RNA (dsRNA) fungal viruses (mycoviruses), including a partitivirus (RnPV1), a quadrivirus (RnQV1), a victorivirus (RnVV1), a mycoreovirus (RnMyRV3), and a megabirnavirus (RnMBV1) in the phytopathogenic fungus Rosellinia necatrix. Expression profiling of RNA silencing-related genes revealed that a dicer-like gene, an Argonaute-like gene, and two RNA-dependent RNA polymerase genes were upregulated by RnMyRV3 or RnMBV1 infection but not by other virus infections or by constitutive expression of dsRNA in R. necatrix. Massive analysis of viral small RNAs (vsRNAs) from the five mycoviruses showed that 19-to 22-nucleotide (nt) vsRNAs were predominant; however, their ability to form duplexes with 3= overhangs and the 5= nucleotide preferences of vsRNAs differed among the five mycoviruses. The abundances of 19-to 22-nt vsRNAs from RnPV1, RnQV1, RnVV1, RnMyRV3, and RnMBV1 were 6.8%, 1.2%, 0.3%, 13.0%, and 24.9%, respectively. Importantly, the vsRNA abundances and accumulation levels of viral RNA were not always correlated, and the origins of the vsRNAs were distinguishable among the five mycoviruses. These data corroborated diverse interactions between encapsidated dsRNA mycoviruses and RNA silencing. Moreover, a green fluorescent protein (GFP)-based sensor assay in R. necatrix revealed that RnMBV1 infection induced silencing of the target sensor gene (GFP gene and the partial RnMBV1 sequence), suggesting that vsRNAs from RnMBV1 activated the RNA-induced silencing complex. Overall, this study provides insights into RNA silencing against encapsidated dsRNA mycoviruses. IMPORTANCEEncapsidated dsRNA fungal viruses (mycoviruses) are believed to replicate inside their virions; therefore, there is a question of whether they induce RNA silencing. Here, we investigated inductions of RNA silencing against encapsidated dsRNA mycoviruses (a partitivirus, a quadrivirus, a victorivirus, a mycoreovirus, and a megabirnavirus) in Rosellinia necatrix. We revealed upregulation of RNA silencing-related genes in R. necatrix infected with a mycoreovirus or a megabirnavirus but not with other viruses, which was consistent with the relatively high abundances of vsRNAs from the two mycoviruses. We also showed common and different molecular features and origins of the vsRNAs from the five mycoviruses. Furthermore, we demonstrated the activation of RNA-induced silencing complex by mycoviruses in R. necatrix. Taken together, our data provide insights into an RNA silencing pathway against encapsidated dsRNA mycoviruses which is differentially induced among encapsidated dsRNA mycoviruses; that is, diverse replication strategies exist among encapsidated dsRNA mycoviruses. RNA silencing is a sequence-specific RNA degradation mechanism that is widely conserved among eukaryotic organisms, including animals, plants, and fungi (1-4). This mechanism is triggered by th...

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“…Esses segmentos são encapsidados em diferentes partículas icosaédricas de capsídeo duplo, com 80 nm de diâmetro (HILLMAN; SUZUKI, 2005;WEI et al, 2004). Todas as espécies relatadas conferem hipovirulência a seus hospedeiros (GHABRIAL et al, 2015).…”

Section: Taxonomia De Micovírusunclassified

Vírus que infectam fungos fitopatogênicos

et al. 2018

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Mycovirus are viruses that infect all taxa of fungi.They are generally cryptic or latent, but they can induce minor or imperceptible changes in hosts. Mycoviruses can interfere with the symptoms induced by phytopathogenic fungi hosts, and in some cases they reduce the virulence of its host. Therefore, they are objects of study since they are potential biocontrol agents and are important tools for knowledge of fungal pathogenesis mechanisms. The aim of this review was to gather literature data concerning general aspects of biology and behavior of mycovirus, focusing some phytopathogenic mycovirus.KEYWORDS: mycovirus; biocontrol; hypovirulence; vegetative incompatibility. RESUMO: Micovírus são vírus que infectam todos os taxa de fungos.São geralmente crípticos (latentes), mas podem causar pequenas ou imperceptíveis alterações no hospedeiro. Nos fungos fitopatogênicos, os vírus podem interferir com os sintomas e, em alguns casos, reduzir a virulência de seu hospedeiro; por esta razão, são objeto de estudo, por serem um potencial agente de biocontrole e por serem ferramentas importantes para o conhecimento sobre os mecanismos de patogênese de fungos. A presente revisão teve o objetivo de reunir os dados de literatura relacionados aos aspectos gerais da biologia e do comportamento dos micovírus presentes em alguns fungos fitopatogênicos. PALAVRAS-CHAVE: micovírus; biocontrole; hipovirulência;incompatibilidade vegetativa.

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Complete nucleotide sequences of genome segments 1 and 3 of Rosellinia anti-rot virus in the family Reoviridae

Cited by 39 publications

References 15 publications

50-plus years of fungal viruses

50-plus years of fungal viruses

Differential Inductions of RNA Silencing among Encapsidated Double-Stranded RNA Mycoviruses in the White Root Rot Fungus Rosellinia necatrix

Vírus que infectam fungos fitopatogênicos

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