Complex assessment of distinct cognitive impairments following ouabain injection into the rat dorsoloateral striatum

Gornicka-Pawlak, Elzbieta; Janowski, Mirosław; Jabłońska, Anna; Sypecka, Joanna; Domanska-Janik, Krystyna

doi:10.1016/j.bbr.2015.03.061

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…On the other hand, animal with B7 and B9 serotonergic nuclei damaged, showed a tendency to worse its response in implicit test, but results did not show significance between the 1 st and 3 rd day of execution. Many disorders seen in patients without an effective treatment have encouraged basic researchers to look for novel approaches in the use of animal models of cognitive impairment, which is important for the understanding of its physiopathology and also the development of new therapies (Gornicka-Pawlak et al, 2015;Dalrymple-Alford et al, 2015;Huijgen and Samson, 2015;Danet et al, 2015). This work suggests that serotonergic nuclei together with Papez's circuit might be an important target in the study and understanding of the mechanisms involved in memory impairment.…”

Section: Discussionmentioning

confidence: 99%

Assessment of serotonergic system in formation of memory and learning

et al. 2017

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We evaluated the involvement of the serotonergic system on memory formation and learning processes in healthy adults Wistar rats. Fifty-seven rats of 5 groups had one serotonergic nuclei damaged by an electric current. Electrolytic lesion was carried out using a continuous current of 2mA during two seconds by stereotactic surgery. Animals were submitted to learning and memory tests. Rats presented different responses in the memory tests depending on the serotonergic nucleus involved. Both explicit and implicit memory may be affected after lesion although some groups showed significant difference and others did not. A damage in the serotonergic nucleus was able to cause impairment in the memory of Wistar. The formation of implicit and explicit memory is impaired after injury in some serotonergic nuclei.Keywords: memory, rat, serotonin, learning, neuronal plasticity. Avaliação do sistema serotonérgico na formação de memória e aprendizagem ResumoAvaliar a participação do sistema serotoninérgico em processos de formação de memória e aprendizagem em ratos Wistar adultos saudáveis. Cinquenta e sete ratos de 5 grupos tinham um núcleo serotoninérgico danificado por uma corrente elétrica. A lesão eletrolítica foi realizada utilizando uma corrente contínua de 2 mA durante dois segundos por cirurgia estereotáxica. Os animais foram submetidos a testes de aprendizagem e memória. Os ratos apresentaram respostas diferentes nos testes de memória, dependendo do núcleo serotoninérgica envolvido. A memória explícita e implícita pode ser afetada após a lesão, embora alguns grupos apresentaram diferença significativa e outros não. A lesão no núcleo serotoninérgico foi capaz de causar danos na memória de Wistar. A formação da memória implícita e explícita é prejudicada após a lesão em alguns núcleos serotoninérgicos.Palavras-chaves: memória, rato, serotonina, aprendizagem, plasticidade neuronal.

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Section: Discussionmentioning

confidence: 99%

Assessment of serotonergic system in formation of memory and learning

et al. 2017

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“…Male Wistar rats weighing around 250 g were used for experiments. Animals were kept in enriched environment housing conditions which was introduced just after receiving rats from animal house, 4 days before brain injury, as previously described in detail (15, 27, 28). Enriched environment cages (70 × 41 × 56 cm) contained beams, platforms, ladders etc.…”

Section: Methodsmentioning

confidence: 99%

“…The stereotaxic ouabain injection into the right dorsolateral striatum (Figure 1A) was made like previously described (15, 27, 28). Briefly, 1.5 μl 5 nmol ouabain (OUA) has been injected with coordinates A: 0.5, L: 3.8, V: 4.7 at a speed 1 μl/min.…”

Section: Methodsmentioning

confidence: 99%

“…The T-maze habit learning task was performed like previously described (28). Briefly, the T-maze was made of black wood.…”

Section: Methodsmentioning

confidence: 99%

“…In our model the ouabain injection into dorsolateral striatum was shown to produce significant motor deficits in walking beam task and changed patterns of exploratory behavior in the open field (27). This focal brain injury also caused the cognitive impairments i.e., habit learning and cognitive flexibility deficits (28). To maximize the effectiveness of cell treatment we used the large groups of experimental animals housed in enriched environment conditions.…”

Section: Introductionmentioning

confidence: 99%

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Intra-arterial Administration of Human Umbilical Cord Blood Derived Cells Inversed Learning Asymmetry Resulting From Focal Brain Injury in Rat

et al. 2019

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Background: Focal brain injury is a leading cause of serious disability significantly worsening patients' quality of life. Such damage disrupts the existing circuits, leads to motor, and cognitive impairments as well as results in a functional asymmetry. To date, there is still no therapy to effectively restore the lost functions. We examined the effectiveness of human umbilical cord blood (HUCB)-derived cells after their intra-arterial infusion following focal stroke-like brain damage. Methods: The model of stroke was performed using ouabain stereotactic injection into the right dorsolateral striatum in rats. Two days following the brain injury 10 7 cells were infused into the right carotid artery. The experimental animals were placed into enriched environment housing conditions to enhance the recovery process. Behavioral testing was performed using a battery of tasks visualizing motor as well as cognitive deficits for 30 days following brain injury. We assessed animal asymmetry while they were moving forward at time of testing in different tasks. Results: We found that intra-arterial infusion of HUCB-derived cells inversed lateralized performance resulting from the focal brain injury at the early stage of T-maze habit learning task training. The inversion was independent from the level of neural commitment of infused cells. The learning asymmetry inversion was observed only under specific circumstances created by the applied task design. We did not found such inversion in walking beam task, vibrissae elicited forelimb placing, the first exploration of open field, T-maze switching task as well as apomorphine induced rotations. Both the asymmetry induced by the focal brain injury and its inversion resulting from cell infusion decreased along the training. The inversion of learning asymmetry was also independent on the range of the brain damage. Conclusions: Intra-arterial infusion of HUCB-derived cells inversed lateralized performance of learning task resulting from focal brain damage. The inversion was not visible in any other of the used motor as well as cognitive tests. The observed behavioral effect of cell infusion was also not related to the range of the brain damage. Our findings contribute to describing the effects of systemic treatment with the HUCB-derived cells on functional recovery following focal brain injury.

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