Complex glycosphingolipids with blood‐group a specificity

Slomiany, Bronislaw L.; Slomiany, Amalia

doi:10.1016/0014-5793(77)80975-7

Cited by 41 publications

(10 citation statements)

References 18 publications

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“…Earlier this sequence has been established in the carbohydrate peptide linkage region of GlcNAc-+Asn-type oligosaccharide chains of glycoproteins [14,15]. The GlcNAcl j 3 G l c N A c sequence was found in A (H) bloodgroup substance from pig gastric much [I61 and the 1-4 isomer of the disaccharide in complex glycosphingolipids from the some source [17]. The anomeric type of linkages were not determined.…”

Section: Discussionmentioning

confidence: 99%

Studies on the Structure and I-Blood-Group Activity of Poly(glycosyl)ceramides

Zdebska

Kościelak

1978

Eur J Biochem

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Employing a modified technique of acetolysis, which allows almost a complete recovery of constituent sugars from poly(glycosyl)ceramides, the glycolipids were found to contain an excess of N-acetylglucosamine over galactose. On the basis of Smith degradation, methylation study, chromium trioxide degradation and the structures of oligosaccharides released from the glycolipids by partial acid hydrolysis, the presence of two types of sugar sequences has been established in poly(glycosy1)ceramides :The repeating unit of poly(glycosy1)ceramides seems to be the GlcNAcfll+3Gal sequence.The specificity of one anti-I serum (Woj) is directed against the non-reducing ending of the first kind of chain. Three other anti-I sera reacted with inner portions of the oligosaccharide chains of the glycolipids.Poly(glycosy1)ceramides are a family of very complex glycosphingolipids of human erythrocyte membranes which may contain up to 59 carbohydrate residues/mol [l]. They exhibit A, B, H and I bloodgroup activities. Previously we proposed tentative structures for these compounds, which were based mainly on their quantitative composition and methylation data. Now we shall describe the results of further structural and immunological studies of poly(glyc0-sy1)ceramides. A preliminary report of these studies has been presented [2]. MATERIALS AND METHODS Glycol@ idsPoly(glycosy1)ceramides were obtained from outdated blood of group 0, which was supplied by Dr J. Ostaszewski of the Warsaw Blood Bank. The isolation

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Section: Discussionmentioning

confidence: 99%

Studies on the Structure and I-Blood-Group Activity of Poly(glycosyl)ceramides

Zdebska

Kościelak

1978

Eur J Biochem

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“…A large number of fucose containing substances, chemically characterized as oligosaccharides, glycoproteins or glycolipids have been isolated from cells, tissues or fluids of various animal species including those of human, bovine, pig, dog and rat [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Activity of 2‐α‐L‐fucosyltransferase in human sera and red cell membranes A study of common ABH blood donors, rare ‘Bombay’ and ‘Parabombay’ individuals

et al. 1977

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“…Aside from differences in the degree of branching, two types of carbohydrate sequences have been distinguished in the region adjacent to ceramidecore. One type was found to contain GlcNAc-(fl1-+3)Gal(fl1-.4)Gal(~1-+4)Glc+Cer and the other contained GlcNac(fl1+4)GlcNAc(/il+3)Gal(/~1-+4)-Glc+Cer [4,6,11]. The flGlcNacl-+4~GlcNAc sequence originally found in carbohydrate chains of sulfated glycoprotein from hog gastric mucosa [ 17,201, has also been recently detected in complex fucolipids of human erythrocyte membrane [2].…”

Section: Disc Us Si 0 Nmentioning

confidence: 99%

“…Controlled periodate oxidation of the fucolipid was performed with 0.015 M sodium metaperiodate in methanol water (1 : 2, v/v) at room temperature for 24 h in the dark [4]. Following reduction with NaBH4 and destruction of excess of borohydride with diluted acetic acid, the reaction mixture was dialyzed against distilled water and subjected to completion of Smith degradation [I 71.…”

Section: Periodutc O~:idationmentioning

confidence: 99%

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Complex Fucolipids of Hog Gastric Mucosa Structure of the Ceramide Eikosahexoside

Slomiany

Galicki

et al. 2005

European Journal of Biochemistry

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The structure of a complex fucolipid from hog gastric mucosa containing twenty sugar residues and exhibiting blood‐group (A+H) activity has been investigated. Based on the results of immunological assays, partial acid hydrolysis, sequential degradation with specific exoglycosidases, oxidation with periodate and chromium trioxide, and permethylation analysis, we suggest that the carbohydrate chain of this fucolipid contains four termini. One of the termini bears βGal1→ 4βGlcNAc disaccharide, two bear blood‐group A determinant and one bears H determinant. Two of the branches, terminated by βGal1 → 4βGlcNAc and blood‐group A determinant, and two terminated by blood‐group A and H determinants, are linked through βGal1→ 4βGlcNAc1 → 3/6 and βGal1 → 4βGlcNAc1→ 4βGlcNAc1→ 3/6 to the galactose residue adjacent to glucosylceramide core.

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Complex glycosphingolipids with blood‐group a specificity

Cited by 41 publications

References 18 publications

Studies on the Structure and I-Blood-Group Activity of Poly(glycosyl)ceramides

Studies on the Structure and I-Blood-Group Activity of Poly(glycosyl)ceramides

Activity of 2‐α‐L‐fucosyltransferase in human sera and red cell membranes A study of common ABH blood donors, rare ‘Bombay’ and ‘Parabombay’ individuals

Complex Fucolipids of Hog Gastric Mucosa Structure of the Ceramide Eikosahexoside

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