Complex protein nanopatterns over large areas via colloidal lithography

Kristensen, Stine H.; Pedersen, Gitte A.; Ogaki, Ryosuke; Bochenkov, Vladimir E.; Nejsum, Lene N.; Sutherland, Duncan S.

doi:10.1016/j.actbio.2013.01.011

Cited by 20 publications

(28 citation statements)

References 39 publications

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“…Proteins were eluted with the elution buffer: 250 mM imidazole in interaction buffer. Ecad-fragment crystallizable (Fc) covers the extracellular domain of Ecad and has previously been shown to be functional in in vitro binding and adhesion studies (30)(31)(32). HEK293 cells expressing Epstein-Barr virus nuclear antigen-1, expressing Ecad-Fc, were obtained from Prof. W. James Nelson.…”

Section: Protein Purificationmentioning

confidence: 99%

The soluble extracellular domain of E‐cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex

Jensen

Pedersen

et al. 2018

The FASEB Journal

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Enteropathogenic Escherichia coli (EPEC) causes watery diarrhea when colonizing the surface of enterocytes. The translocated intimin receptor (Tir):intimin receptor complex facilitates tight adherence to epithelial cells and formation of actin pedestals beneath EPEC. We found that the host cell adherens junction protein E-cadherin (Ecad) was recruited to EPEC microcolonies. Live-cell and confocal imaging revealed that Ecad recruitment depends on, and occurs after, formation of the Tir:intimin complex. Combinatorial binding experiments using wild-type EPEC, isogenic mutants lacking Tir or intimin, and E. coli expressing intimin showed that the extracellular domain of Ecad binds the bacterial surface in a Tir:intimin-dependent manner. Finally, addition of the soluble extracellular domain of Ecad to the infection medium or depletion of Ecad extracellular domain from the cell surface reduced EPEC adhesion to host cells. Thus, the soluble extracellular domain of Ecad may be used in the design of intervention strategies targeting EPEC adherence to host cells.-Login, F. H., Jensen, H. H., Pedersen, G. A., Amieva, M. R., Nejsum, L. N. The soluble extracellular domain of E-cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex.

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Section: Protein Purificationmentioning

confidence: 99%

The soluble extracellular domain of E‐cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex

Jensen

Pedersen

et al. 2018

The FASEB Journal

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“…Similar to the previous section, relevant examples of nanofabrication techniques using different approaches, such as optical (nanometer-scale optical photolithography [49,58,64–65], nanoimprint lithography [66]), etching (focused-ion beam [67] and electron-beam nanolithography [68]), electrical (electrospinning [69–72]), mechanical (nanoskiving [46,73], nanoimprint lithography [66]) and colloidal (colloidal lithgraphy [74–75]) are given here.…”

Section: Reviewmentioning

confidence: 99%

“…The size of the colloids is tunable and determines the resolution of the pattern. Resolutions of few tens of nanometers can be achieved [74–75]. …”

Section: Reviewmentioning

confidence: 99%

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

Greiner¹,

Sales²,

Chen³

et al. 2016

Beilstein J. Nanotechnol.

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The extracellular environment of vascular cells in vivo is complex in its chemical composition, physical properties, and architecture. Consequently, it has been a great challenge to study vascular cell responses in vitro, either to understand their interaction with their native environment or to investigate their interaction with artificial structures such as implant surfaces. New procedures and techniques from materials science to fabricate bio-scaffolds and surfaces have enabled novel studies of vascular cell responses under well-defined, controllable culture conditions. These advancements are paving the way for a deeper understanding of vascular cell biology and materials–cell interaction. Here, we review previous work focusing on the interaction of vascular smooth muscle cells (SMCs) and endothelial cells (ECs) with materials having micro- and nanostructured surfaces. We summarize fabrication techniques for surface topographies, materials, geometries, biochemical functionalization, and mechanical properties of such materials. Furthermore, various studies on vascular cell behavior and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on potential interesting future studies.

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“…More importantly, binary colloidal assemblies are capable of producing highly ordered structures, which can be readily controlled by the particle size, size ratio of the particles and number of small particles with respect to large particles. This approach has mostly only been used for the manufacture of masks to generate surface patterns when combined with other deposition methods and subsequent removal of the particles [21][22][23][24]. However, binary colloidal assemblies have recently also been proposed as cell culture substrates [25].…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach to Quantitatively Assess the Uniformity of Binary Colloidal Crystal Assemblies

et al. 2016

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Colloidal self-assembly into highly ordered binary systems represents a versatile and inexpensive approach to generate well defined surface topographical features with submicron resolution. In addition, the use of surface-functionalized particles where each particle bears a different surface functionality enables the generation of highly resolved surface chemical patterns. Such topographical, as well as chemical features, are of great interest in biomaterials science particularly in the context of investigating and controlling the cellular response. While colloidal crystals have been used to generate a wide range of surface patterns, it has not been possible until now to quantitatively describe the degree of uniformity within such systems. In the present work we describe a novel approach to quantitatively assess the uniformity within binary colloidal assemblies based on image processing methods, primarily the Circular Hough Transform and distance calculations. We believe that the methodology presented here will find broad application in the field of colloidal crystals to quantitatively describe the integrity and homogeneity of assemblies.

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Complex protein nanopatterns over large areas via colloidal lithography

Cited by 20 publications

References 39 publications

The soluble extracellular domain of E‐cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex

The soluble extracellular domain of E‐cadherin interferes with EPEC adherence via interaction with the Tir:intimin complex

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

A Novel Approach to Quantitatively Assess the Uniformity of Binary Colloidal Crystal Assemblies

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