Complex-specific immunoglobulin M antibody patterns in humans infected with alphaviruses

Calisher, Charles H.; el-Kafrawi, A O; Mahmud, M. I. A.; Rosa, A.P.A. Travassos da; Bartz, C. R.; Brummer-Korvenkontio, M; Haksohusodo, Suwardji; Wuryadi, Suharyono

doi:10.1128/jcm.23.1.155-159.1986

Cited by 79 publications

(23 citation statements)

References 12 publications

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“…This is reminiscent of responses to some other groups of antigenically related viruses (e.g. alphaviruses) where cross-reactive IgM responses have been documented [Calisher et al, 1986]. The viral antigens in the CMV IgM ELISA assay may be different to those relevant in the ACIF assay to detect rising antibody titres to CMV.…”

Section: Discussionmentioning

confidence: 96%

Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients

et al. 1997

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Diagnosis of significant infections by human herpesvirus 6 (HHV6) and 7 (HHV7) in transplant patients has proved difficult because both viruses are ubiquitous and can cause persistent infections in their hosts. The significance of viral DNA detected in peripheral blood leukocytes (PBLs; DNAemia) by PCR is therefore unclear. The interpretation of serological results is complicated by the fact that both primary and secondary infections with other herpesviruses may be associated with a concurrent antibody response to HHV6. Fifty-four renal allograft recipients were studied prospectively and their serological response to HHV6, HHV7 and CMV were compared with the detection of viral DNAemia from the homologous and heterologous viruses. Serum and heparinished blood samples were collected prospectively from 54 renal allograft recipients. DNA was extracted from PBLs and tested for the presence of HHV6, HHV7 and CMV DNA by PCR. Antibodies to HHV6 and HHV7 were measured by an indirect immunofluorescence test and to CMV by an anticomplement immunofluorescence (ACIF) test. CMV IgM antibodies were detected by a commercial enzyme immunoassay. CMV and HHV7 DNAemia were each significantly associated with serological responses to the homologous virus but no such association was found for HHV6 DNAemia. However, patients with consecutively positive DNAemia to any of the viruses (including HHV6) were more likely to have a homologous serological response. Patients who had detectable CMV IgM without a concurrent rise in CMV antibodies were significantly less likely to have CMV DNAemia (odds ratio = 0.16; 95% CI 0.02-0.9). CMV IgM antibodies may be associated with HHV6 or HHV7 DNAemia (odds ratio 2.3; 95% CI 0.5-15). This serological profile may reflect a crossreactive response to HHV6, HHV7 or other herpesviruses. CMV IgM should not be used in isolation for the diagnosis of CMV infection or disease in this group of patients.

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Section: Discussionmentioning

confidence: 96%

Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients

et al. 1997

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“…In a broader context, analysis of IgM response in humans with alphaviruses infection has revealed the potential usefulness of IgM as a diagnostic tool especially during endemics. 53 Indeed, IgM-based ELISA has been developed for CHIKV serodiagnosis of adult patients infected with La Reunion strain. 54 At present, there are no prior studies done on understanding the use of CHIKV-specific IgM as an immunotherapy in patients and animal models.…”

Section: Discussionmentioning

confidence: 99%

A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis

Lam

Min

Phuektes

et al. 2015

mAbs

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Keywords: envelope 2 protein, epitope, monoclonal antibody, virus pathogenesis Chikungunya virus (CHIKV) is a medically important human viral pathogen that causes Chikungunya fever accompanied with debilitating and persistent joint pain. Host-elicited or passively-transferred monoclonal antibodies (mAb) are essential mediators of CHIKV clearance. Therefore, this study aimed to generate and characterize a panel of mAbs for their neutralization efficacy against CHIKV infection in a cell-based and murine model.To evaluate their antigenicity and neutralization profile, indirect enzyme-linked immunosorbent assay (ELISA), an immunofluorescence assay (IFA) and a plaque reduction neutralization test were performed on mAbs of IgM isotype. CHIKV escape mutants against mAb 3E7b neutralization were generated, and reverse genetics techniques were then used to create an infectious CHIKV clone with a single mutation. 3E7b was also administered to neonate mice prior or after CHIKV infection. The survival rate, CHIKV burden in tissues and histopathology of the limb muscles were evaluated. Both IgM 3E7b and 8A2c bind strongly to native CHIKV surface and potently neutralize CHIKV replication. Further analyses of 3E7b binding and neutralization of CHIKV single-mutant clones revealed that N218 of CHIKV E2 protein is a potent neutralizing epitope. In a pre-binding neutralization assay, 3E7b blocks CHIKV attachment to permissive cells, possibly by binding to the surface-accessible E2-N218 residue. Prophylactic administration of 3E7b to neonate mice markedly reduced viremia and protected against CHIKV pathogenesis in various mice tissues. Given therapeutically at 4 h post-infection, 3E7b conferred 100% survival rate and similarly reduced CHIKV load in most mice tissues except the limb muscles. Collectively, these findings highlight the usefulness of 3E7b for future prophylactic or epitope-based vaccine design.

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“…There is too little information relating to IgM antibody responses in anamnestic a1phavirus infections in humans to determine whether these three apparent dual infections with Ross River and Barmah Forest viruses could have been due to a primary infection with one virus and an anamnestic response to antigens shared between this virus and one causing an earlier, perhaps subclinical, infection. However the low levels of cross-reactivity between alphavirus IgM antibodies makes this unlikely.,, 22 As well as collecting the data necessary to complete a comprehensive picture of clinical Barmah Forest virus disease, tile interval from infection to the onset of symtoms needs to be determined, as does the duration of viraemia. A knowledge of the duration of viraemia and of the incidence in the community (estimated to be 0.23% of the population per annum in Queensland) may indicate whether the continuous transmission of Barmah Forest virus requires a non-human host or whether human-mosquito-human cycles are liIely to be self-sustaining.…”

Section: Discussionmentioning

confidence: 99%

Clinical and subclinical Barmah Forest virus infection in Queensland

Phillips

Murray

Aaskov

et al. 1990

Medical Journal of Australia

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Barmah Forest virus is a mosquito‐borne agent (alphavirus) reported to cause both clinical and subclinical infections in New South Wales. This report describes 29 cases of clinical Barmah Forest virus infection diagnosed between July 1988 and March 1989 (21 from Queensland, six from New South Wales and two from Victoria) and provides evidence of extensive subclinical infection with this virus (0.23% of the population per annum) throughout Queensland. It also includes a description of the first isolation of Barmah Forest yirus from a patient. Data obtained in the course of the study suggest that Barmah Forest virus infections may not be diagnosed correctly in many instances because of the similarity of the symptoms of this disease to those of epidemic polyarthritis and the small number of laboratories providing the necessary serological services.

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Complex-specific immunoglobulin M antibody patterns in humans infected with alphaviruses

Cited by 79 publications

References 12 publications

Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients

Correlation between the detection of viral DNA by the polymerase chain reaction in peripheral blood leukocytes and serological responses to human herpesvirus 6, human herpesvirus 7, and cytomegalovirus in renal allograft recipients

A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis

Clinical and subclinical Barmah Forest virus infection in Queensland

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