Complex vertebral malformation in Holstein cows in Japan and its inheritance to crossbred F1 generation

Ghanem, Mohamed Elshabrawy; Akita, Masashi; Suzuki, Toshihiko; Kasuga, Asako; Nishibori, Masahide

doi:10.1016/j.anireprosci.2007.05.006

Cited by 24 publications

(25 citation statements)

References 12 publications

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“…Although the frequency of CVM carriers found in this study was very low, higher frequencies of CVM carriers were reported by Meydan et al (2010) in Turkish Holstein cows (3.4%), Ghanem et al (2008) in Japanese Holstein cows (13%), and Chu et al (2008) in Chinese Holstein bulls (15%). The frequency of carriers for CVM in some countries has been reported at alarming levels, e.g., Swedish Holsteins (23%) (Berglund et al, 2004) and Polish Holstein-Friesian bulls (24.79%) (Ruść and Kamiński, 2007).…”

Section: Discussionmentioning

confidence: 81%

Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in Brazilian Girolando cattle

Paiva¹,

Fonseca²,

Pinto³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Among the various hereditary diseases that have been widely studied in dairy cattle, bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine-5-monophosphate synthase (DUMPS), and complex vertebral malformation (CVM) are noteworthy because of their high impact on overall herd productivity as a consequence of increased calf mortality. The aim of this study was to verify the frequency of carriers of BLAD, CVM, and DUMPS mutant alleles in cows and bulls from the National Girolando Progeny Test carried out in Brazil by using polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific PCR assays. A total of 777 animals were genotyped for BLAD, 783 for CVM, and 122 for DUMPS. The frequencies of carriers for BLAD and CVM were 0.77 and 1.53%, respectively, whereas no carriers of DUMPS were observed.

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Section: Discussionmentioning

confidence: 81%

Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in Brazilian Girolando cattle

Paiva¹,

Fonseca²,

Pinto³

et al. 2013

Genet. Mol. Res.

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“…However, in a recent report [21] 150 CVM carriers (24.8%) were identified from 605 Polish Holstein sires. In another recent study performed in 200 Japanese Holstein cows, 26 animals were CVM carriers (13.0%) [58]. Hence, in some Holstein populations, the frequency of CVM carriers still seems to be high.…”

Section: Discussionmentioning

confidence: 99%

Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey

2010

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BackgroundBovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID) are autosomal recessive hereditary disorders, which have had significant economic impact on dairy cattle breeding worldwide. In this study, 350 Holstein cows reared in Turkey were screened for BLAD, DUMPS, CVM, BC and FXID genotypes to obtain an indication on the importance of these defects in Turkish Holsteins.MethodsGenomic DNA was obtained from blood and the amplicons of BLAD, DUMPS, CVM, BC and FXID were obtained by using PCR. PCR products were digested with TaqI, AvaI and AvaII restriction enzymes for BLAD, DUMPS, and BC, respectively. These digested products and PCR product of FXID were analyzed by agarose gel electrophoresis stained with ethidium bromide. CVM genotypes were detected by DNA sequencing. Additionally, all genotypes were confirmed by DNA sequencing to determine whether there was a mutant allele or not.ResultsFourteen BLAD, twelve CVM and four FXID carriers were found among the 350 Holstein cows examined, while carriers of DUMPS and BC were not detected. The mutant allele frequencies were calculated as 0.02, 0.017, and 0.006 for BLAD, CVM and FXID, respectively with corresponding carrier prevalence of 4.0% (BLAD), 3.4% (CVM) and 1.2% (FXID).ConclusionThis study demonstrates that carriers of BLAD, CVM and FXID are present in the Turkish Holstein population, although at a low frequency. The actual number of clinical cases is unknown, but sporadic cases may appear. As artificial insemination is widely used in dairy cattle breeding, carriers of BLAD, CVM and FXID are likely present within the population of breeding sires. It is recommended to screen breeding sires for these defective genes in order to avoid an unwanted spread within the population.

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“…The use of CVM carriers will inevitably result in economic losses in the management of dairy cattle herds Thus, many countries have carry out the detection of CVM and report the distribution of CVM carriers (Berglund et al, 2004;Thomsen et al, 2006;Rusc and Kaminski, 2007;Ghanem et al, 2008;Chu et al, 2008). The frozen semen and embryos of our country are imported from the United States and Denmark and other countries.…”

Section: Several Methods Used To Detect Cvm Have Been Reportedmentioning

confidence: 99%

“…For instance, PCR-single-stranded conformational polymorphism (PCR-SSCP) (Rusc and Kaminski, 2007;Chu et al, 2008), allele-specific PCR (AS-PCR) (Bendixen et al, 2002;Ghanem et al, 2008), microsatellite symbol method (Berglund et al, 2004) and PCR primerintroduced restriction analysis (PCR-PIRA) (Kanae et al, 2005). However, CRS-PCR, applied in this study, is a simple and efficient technique to identify single nucleotide polymorphism (SNP) genotypes, in which one or more mismatch bases are introduced in a primer to create a re-, in which one or more mismatch bases are introduced in a primer to create a rene or more mismatch bases are introduced in a primer to create a reintroduced in a primer to create a reed in a primer to create a restriction site by combining the SNP site after PCR.…”

Section: Several Methods Used To Detect Cvm Have Been Reportedmentioning

confidence: 99%

Identification of complex vertebral malformation carriers in Holstein cattle in south China

Wang

Tong²,

Hu³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Complex vertebral malformation (CVM) is a recently described monogenic autosomal recessive hereditary defect of Holstein dairy cattle that causes premature birth, aborted fetuses and stillborn calves. Guanine is substituted by thymine (G>T) in the solute carrier family 35 member A3 gene (SLC35A3). A valine is changed to a phenylalanine at position 180 of uridine 5'-diphosphate-N-acetylglucosamine transporter protein. CVM is expected to occur in many countries due to the widespread use of sire semen. We developed a created restriction site PCR (CRS-PCR) method to diagnose CVM in dairy cows. This was tested on 217 cows and 125 bulls selected randomly from a Holstein cattle population in south China. Five Holstein cows and five Holstein bulls were identified to be CVM carriers; the percentages of CVM carriers were estimated to be 2.3, 4.0 and 2.9% in the cows, bulls and entire Holstein cattle sample, respectively.

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Complex vertebral malformation in Holstein cows in Japan and its inheritance to crossbred F1 generation

Cited by 24 publications

References 12 publications

Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in Brazilian Girolando cattle

Incidence of bovine leukocyte adhesion deficiency, complex vertebral malformation, and deficiency of uridine-5-monophosphate synthase carriers in Brazilian Girolando cattle

Screening for bovine leukocyte adhesion deficiency, deficiency of uridine monophosphate synthase, complex vertebral malformation, bovine citrullinaemia, and factor XI deficiency in Holstein cows reared in Turkey

Identification of complex vertebral malformation carriers in Holstein cattle in south China

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