Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil

Mouez, Mamdouh. Abdel; Nasr, Maha; Abdel-Mottaleb, Mona M.A.; Geneidi, Ahmed S.; Mansour, Samar

doi:10.1016/j.ijbiomac.2016.09.027

Cited by 64 publications

(42 citation statements)

References 37 publications

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“…Purification of PEVs from the non-encapsulated drug was done by exhaustive dialysis, in which PEVs formulae were placed in a dialysis tubing (Dialysis membrane Spectra/Por 12.000–14.000 molecular weight) 31 . The dialysis was carried out against 1000 ml distilled water for 2 hours 7 , which was found appropriate for the removal of the non-entrapped DMH in the medium.…”

Section: Methodsmentioning

confidence: 99%

Intranasally administered in situ gelling nanocomposite system of dimenhydrinate: preparation, characterization and pharmacodynamic applicability in chemotherapy induced emesis model

Barakat

Nasr

Ahmed

et al. 2017

Sci Rep

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The aim of the current manuscript was to test the applicability of a nanocomposite system of penetration enhancer vesicles (PEVs) within polymeric in situ forming gel network composed of poloxamer and hyaluronic acid for the intranasal delivery of the antiemetic dimenhydrinate (DMH). PEVs were prepared using phospholipids and labrasol/transcutol/PEG 400 as penetration enhancers, and characterized for entrapment efficiency (EE%), particle size, zeta potential and morphology. The nanocomposite in situ forming gel system was characterized for its sol-gel temperature, viscosity and mucoadhesiveness, and was pharmacodynamically tested on a cisplatin induced emesis model in rats in terms of food, water, kaolin intake and stomach weight content. The selected PEVs formula displayed EE% of 83% for DMH, particle size of 121 nm and a surface charge of 0.83 mV. The selected nanocomposite in situ gelling formula showed a viscosity of 2.13 Pa.S, mucoadhesive force of 0.62 N and DMH controlled release over 6 hours. The pharmacodynamic study showed the superiority of the nanocomposite in situ gelling formula; being administered at a lower dose than the oral marketed formula. The described nanocomposite system proved to be successful for the intranasal delivery of DMH, thus presenting a promising delivery modality for similar antiemetics.

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Section: Methodsmentioning

confidence: 99%

Intranasally administered in situ gelling nanocomposite system of dimenhydrinate: preparation, characterization and pharmacodynamic applicability in chemotherapy induced emesis model

Barakat

Nasr

Ahmed

et al. 2017

Sci Rep

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“…A computerized inspection system is used for the process. The Zeta potential is a measure of colloidal suspension stability 22,23 .…”

Section: Vesicles Shape Size and Zeta Potentialmentioning

confidence: 99%

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2019

IJPSR

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The solar UV radiation is the major trigger factor that causes skin cancer. Nearly 65% of cases of melanoma occurs dues to high exposure to the UV radiation. It also accounts for 90% of the nonmelanoma skin cancers also referred to as NMSC also including the cases of basal cell carcinoma (BCC) and the squamous cell carcinoma (SCC). The rate of malignancy in other cases has seemed to get reduced but this is not the situation in case of NMSC, which seemed to have increased and also affect people of younger ages. Cases of NMSC can be accounted for 15,000 deaths in the USA alone. This is surely one of the major health concerns of the nation also. In order to find a solution to this problem a new form of drug delivery system using the specific ligand that has receptor on the surface or the target cells is being proposed. The current discussion deals with the making of the mannosylated quercetin loaded liposomes that can be used for targeting cancerous cells. The methods of transmission electron microscopy (TEM) used for the surface morphology. The MTT assay was done against two different cell lines so that their cancer-preventive or curative properties can be assured. The research, hence establishes the fruitful development of Mannosylated Quercetin loaded liposomes (MA-QuLps) for the targeting of the skin cancer cells.

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“…Cationic STa liposomes were prepared using the thin film hydration method [21,22]. Briefly, 90 mg soyabean lecithin and 10 mg cationic lipid (DOTAP) were dissolved in 4 mL chloroform and 2 mL methanol, which were evaporated using a rotary evaporator (Janke and Kunkel, Germany) at 50ºC to form a thin lipid film on the wall of the flask.…”

Section: Preparation Of Cationic Nanoliposomes Loaded With Stamentioning

confidence: 99%

“…The morphological features of the cationic STa loaded nanoliposomes were examined using transmission electron microscopy (Jeol, JEM 1010, Tokyo, Japan), after allowing the vesicular sample to be adsorbed onto a carbon coated grid and become negatively stained using 2% uranyl acetate solution [12,22,23].…”

Section: Morphology Of the Cationic Sta Loaded Nanoliposomesmentioning

confidence: 99%

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Novel Heat-Stable Enterotoxin (STa) Immunogen Based on Cationic Nanoliposomes: Preparation, Characterization and Immunization

Aref¹,

Nasr²,

Osman³

2017

J Vaccines Vaccin

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The novelties encountered in the field of formulation have provided promising solutions for the problematic vaccine development of hapten molecules. In the current study, the novel preparation of a cationic nanoliposomal immunogen of the heat stable enterotoxin (STa) was reported. STa was produced from clinically ETEC isolate of diarrheic neonatal calves and purified using RP-HPLC. STa was loaded into the cationic vesicles which were characterized for their particle size, surface charge, morphology, STa loading, and stability. The STa loaded cationic nanoliposome was used for mice immunization and the generation of STa antibody was monitored using ELISA. Results displayed the spherical nature of the STa loaded vesicles, their suitable size and homogeneity represented by a particle size of 228.1 nm and a PDI of 0.202. The surface charge of the STa nanoliposomes was +29.9, demonstrating sufficient stability during refrigeration storage. The STa loaded cationic nanoliposome was able to elicit specific STa antibody response, and to confer effective protection against STa challenge in mice. The STa antibody binding titer and neutralization capacity were 10 5 and 10 4 mouse units/ml serum, respectively. The developed system is a one-step procedure, which overcomes the disadvantage of the complexity of generation of the hapten-carrier conjugate. In conclusion, the developed STa-cationic nanoliposomal immunogen is feasible and has the potential to improve effectiveness against ETEC, suggesting its applicability in preventing the harmful effects of ETEC infection in neonatal calves.

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Composite chitosan-transfersomal vesicles for improved transnasal permeation and bioavailability of verapamil

Cited by 64 publications

References 37 publications

Intranasally administered in situ gelling nanocomposite system of dimenhydrinate: preparation, characterization and pharmacodynamic applicability in chemotherapy induced emesis model

Intranasally administered in situ gelling nanocomposite system of dimenhydrinate: preparation, characterization and pharmacodynamic applicability in chemotherapy induced emesis model

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Novel Heat-Stable Enterotoxin (STa) Immunogen Based on Cationic Nanoliposomes: Preparation, Characterization and Immunization

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