Composition and antioxidant capacity of the essential oil of leaves ofVitex megapotamica(Sprengel) Moldenke

Abstract

This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Vitex megapotamica. Gas chromatography-mass spectrometry analysis resulted in the detection of 27 components, representing 92.36% of the total oil composition. The main components in the oil were butylated hydroxytoluene (BHT) (34.17%), phytol (12.66%), α-caryophyllene (11.84%), δ-elemene (10.65%), β-caryophyllene (7.82%), γ-elemene (4.24%) and germacrene D (2.82%). The antioxidant activity of the oil was evaluated in terms of their free-radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl. The oil showed percentage inhibition of 35.62% and 75.25% at concentrations of 76 and 101.6 mg mL(-1), respectively. BHT (36.30%) was also determined by HPLC-DAD in the hexane fraction from the leaves. To the best of our knowledge, this is the first study of the composition and antioxidant activity of the essential oil of the species V. megapotamica.

HPLC Analysis of Phenolics Compounds and Antioxidant Capacity of Leaves of Vitex megapotamica (Sprengel) Moldenke

“…A phytochemical screening of tarumã revealed the presence of anthocyanin glycosides, tannins, catechins, flavonols, xanthones, triterpenoid steroids, cardioactive glycosides, coumarins and organic acids [12]. Recently, the chemical composition of the essential oil of V. megapotamica leaves was verified, proving their antioxidant activity [13]. Therefore, this study aimed to quantify total polyphenols, flavonoids and tannins in the crude extract and dichloromethane, ethyl acetate and n-butanol fractions from leaves of V. megapotamica.…”

Essential Oils and Biological Activities of the Genus Vitex (Lamiaceae) – A Review

“…In addition, the oxygenated monoterpenes were identified in V. cymosa (β-copaene-4-α-ol) (Leitao et al, 1999), V. obovata (1,8-cineole) (Nyiligira et al, 2004), and V. zeyheri (1,8-cineole) (Nyiligira et al, 2004) essential oils. On the other hand, another group of components that presents in Vitex essential oils were butylated hydroxytoluene and methyl linoleate, which were identified in the oils of V. megapotamica (De Brum et al, 2013) and V. kwangsiensis (Tian and Liu, 2018). It must be mentioned here that butylated hydroxytoluene is not a natural compound but an impurity.…”

“…Besides, the oil showed a dose-response relationship in β-carotene oxidation with the best activity at 500 μg/mL (Vale et al, 2019). De Brum et al, (2013) reported the V. megapotamica essential oil showed percentage inhibition of 35.62% and 75.25% at concentrations of 76 and 101.6 mg/mL against DPPH free-radical scavenging assay. However, this report should be read with caution since the main component in the oil is an antioxidant added to diethyl ether for protection.…”

Essential Oil Composition, Antioxidant and Antimicrobial Activities of Guazuma Ulmifolia from Brazil

“…Many in vitro studies have addressed the antioxidant and radicalscavenging properties of essential oils [1,3,16,17]. In particular, DPPH radical is widely used for quickly assessing the ability of antioxidants to transfer labile H atoms to radicals [18].…”

“…Ethanol was used to calibrate the spectrophotometer. The test was performed in triplicate and the calculation of the antioxidant activity followed the equation: % Inhibition=[(A 0 -A 1 )/A 0 ]×100, where A 0 was the absorbance of the control sample (without essential oil) and A 1 was the absorbance in the presence of the sample [17].…”