Composition and Behavior of Head Membrane Lipids of Fresh and Cryopreserved Boar Sperm

Buhr, M.M.; Curtis, E. F.; Kakuda, N.

doi:10.1006/cryo.1994.1028

Cited by 110 publications

(69 citation statements)

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“…Boar sperm membranes possess discrete domains of differing membrane fluidity, which is significantly affected by temperature [1][2]. Moreover, the sperm membranes appear to be the primary site of damage induced by reduced temperature and alterations in their molecular composition can impair the fertilization processes [3].…”

Section: Introductionmentioning

confidence: 99%

“…Several authors demonstrated that a fluorescent membrane probe, 1-anilinonaphthalene-8-sulfonic acid (1,, could be used to study the interactions of egg yolk components with ram or bovine spermatozoa [13][14]. It has been confirmed that ANS is hardly fluorescent in aqueous solutions, but becomes strongly fluorescent in apolar, organic solvents or when bound to hydrophobic molecules [15].…”

Section: Introductionmentioning

confidence: 99%

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mentioning

confidence: 99%

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Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.

Fraser

Zasiadczyk²,

Strzeżek³

2010

Folia Histochem Cytobiol.

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Abstract:The interactions of a fluorescent membrane probe, 1-anilinonaphthalene-8-sulfonic acid (1,, with boar spermatozoa were followed through the use of lipoprotein fraction of ostrich egg yolk (LPFo). Semen samples, extended in Kortowo 3 (K3) extender, were supplemented with 2% or 5% LPFo and stored for 3h at 16°C. Additionally, cold shocktreated spermatozoa (1h at 4°C) were stored in K3 extender supplemented with LPFo for 3h at 16°C. In each boar, the fluorescent enhancement of ANS was observed in K3-extended semen supplemented with LPFo, prior to storage. Following storage, there was a significant increase in LPFo-ANS fluorescence, particularly in the sperm membrane overlying the head and midpiece regions. There were significant differences among the boars with respect to the sperm populations defined by the LPFo-ANS fluorescence. Sperm viability was not significantly affected during the storage period. Furthermore, the proportions of spermatozoa defined by the different patterns of LPFo-ANS fluorescence were low and remained unchanged after storage of cold shock-treated spermatozoa with 2% or 5% LPFo, suggesting irreversible damage to the sperm membrane architecture. These findings indicate that the ANS fluorescent probe could be used to shed more light on the nature of the interactions between LPFo and sperm membrane following semen preservation. Such valuable information could contribute to the development of an optimal protocol for cryopreservation of boar semen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.

Fraser

Zasiadczyk²,

Strzeżek³

2010

Folia Histochem Cytobiol.

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“…A membrana espermática apresenta uma composição mista de fosfolipídios que pode diferir de espécie para espécie, além da temperatura de transição de fase ser variável para cada tipo de fosfolipídio (Buhr et al, 1994). Durante o resfriamento, a separação lateral de fases pode ocorrer em função disso, há aumento da permeabilidade da membrana com perda de cátions e enzimas, redução da atividade enzimática e perturbações nos processos de difusão controlados pela membrana (De Leeuw et al, 1991).…”

Section: Introductionunclassified

“…No entanto, o espermatozoide suíno é sensível a temperaturas inferiores a 15°C, abaixo da qual há redução da motilidade . Sabe-se que na faixa de temperatura de 25ºC a 5ºC ocorre a redução da fluidez dos lipídios da membrana do espermatozoide suíno, o que poderia explicar sua maior sensibilidade ao resfriamento (Buhr et al, 1994). Weber (1989), ao estudar o armazenamento do sêmen suíno a 5°C, verificou que a colocação direta a 5°C causa queda brusca da motilidade e do percentual de acrossomas normais.…”

Section: Introductionunclassified

Fontes de óleo e níveis de suplementação de vitamina E na ração sobre a qualidade do sêmen suíno acondicionado a 17 e 5ºC

et al. 2010

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Oil sources and levels of vitamin E supplementation in the diet on the quality of boar semen cooled at 17 and 5°CABSTRACT -The objective of this study was to evaluate the effect of the addition of oil sources and levels of vitamin E supplementation in the rations on the characteristics of boar semen cooled at 17 and 5ºC. It was used twenty-four Dalboar 85 boars distributed in a complete random design, in a 2 × 3 factorial scheme, with two sources of oil (soybean and salmon) and three levels of antioxidant (150, 300 and 450 vitamin E mg/kg). For animals under supplementation with salmon oil, the motility and hypoosmotic swelling test (HOST) of sperm after 24, 48, and 72 hours was higher than the ones observed in animals fed soybean oil ration. Salmon oil increased the vigor in both temperatures evaluated after 24 and 48 hours. The occurence of total morphological abnormalities was higher in the semen of animals fed soybean oil and cooled at 17ºC while in the lowest cooling temperature (5ºC) there was no difference among animals under supplementation. The abnormal morphology in the semen at 5ºC was higher than in the semen cooled at 17ºC. Salmon oil improves the sperm characteristics of the boar semen cooled at 17 and 5ºC from 24 to 48 hours.

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Capacitation-like changes occur in mouse spermatozoa cooled to low temperatures

Fuller

Whittingham

1997

Mol. Reprod. Dev.

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Composition and Behavior of Head Membrane Lipids of Fresh and Cryopreserved Boar Sperm

Cited by 110 publications

References 0 publications

Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.

Interactions of egg yolk lipoprotein fraction with boar spermatozoa assessed with a fluorescent membrane probe.

Fontes de óleo e níveis de suplementação de vitamina E na ração sobre a qualidade do sêmen suíno acondicionado a 17 e 5ºC

Capacitation-like changes occur in mouse spermatozoa cooled to low temperatures

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