The aim of the study was to investigate the variation in nutritional composition, antioxidant, and antibacterial activities of tomatoes grown in fly ash blended soils, i.e.,T1 (soil 100% + 0% fly ash), T2 (soil 75%+ 25% fly ash), and T3 (soil 50% + 50% fly ash) soils. The tomato samples were harvested and screened for their proximate analysis, mineral composition, vitamin C contents, phenolic profile, and antioxidant and antibacterial activities. Results showed that T3 soil crop has high proximate composition, vitamin C, and phenolic contents as compared with T2 and T1 soil crops. Furthermore, significant variation in the elements analysis was observed in the crops grown in T1, T2, and T3 soils. T3 soil crop exhibited better antioxidant activity in term of total phenolic contents (TPC), total flavonoids contents (TFC), DPPH radical scavenging capacity, and ferric reducing potential as compared with T2 and T1 soil crops. Similarly, maximum inhibition zones were observed for T3 soil crop when tested for Staphylococcus aureus isolate 1 and 3 and methicillin-resistant Staphylococcus aureus (MRSA). In conclusion, the crop grown in T3 blended soil had high antioxidant and antibacterial potentials and better nutritional composition.