Composition characterization and biological activity study of Achillea vermicularis extract and extract‐loaded nanoparticles

Abstract

Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High‐performance liquid chromatography diode array detector (HPLC‐DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8‐hydroxy salvigenin component.

New Thiazole Derivatives: Carbonic Anhydrase I–II and Cholinesterase Inhibition Profiles, Molecular Docking Studies

“…[3,4] Among the 12 hCA isoforms in the human body, hCA I and hCA II, in particular, play a role in epilepsy treatment as well as in other physiologically vital processes such as respiration, pH balance, electrolyte secretion, pathophysiology, and progression of vision loss in patients with diabetes and glaucoma patients. [5][6][7][8][9] Despite these innovations, drugs that inhibit hCA isoenzymes have many side effects. Owing to the multitude of known side effects, recent attention has been focused on the discovery of new alternative hCA inhibitors.…”