Compositional analysis of soybean event IND-ØØ41Ø-5

Abstract

Soybean ( Glycine max L.) is the world’s largest source of protein feed and the second largest source of vegetable oil. Water restriction is the main limiting factor to achieve maximum soybean yields. Therefore, development of varieties that maintain yield under environmental stresses is a major objective of soybean breeding programs. The HaHB4 ( Helianthus annuus homeobox 4) gene from sunflower encodes for a transcription factor involved in the plant´s tolerance to environmental stress. The introduction of HaHB4 in soybean led to the development of event IND-ØØ41Ø-5 (HB4® soybean), which displayed higher yield in environments having low productivity potential, compared with the parental control variety. Compositional analyses of soybean event IND-ØØ41Ø-5 were conducted both in Argentina and the United Sates. A total of 44 components were analyzed in grain and 9 components in forage. Based on the results of these studies it was concluded that soybean event IND-ØØ41Ø-5 was compositionally equivalent to its non-transgenic parental control.

Biofortification to avoid malnutrition in humans in a changing climate: Enhancing micronutrient bioavailability in seed, tuber, and storage roots

“…Equally important observations include the idea that seed chemistry was alike between GR2E and the near-isogenic control except for β-carotene and related carotenoids ( Mallikarjuna Swamy et al., 2019 ), suggesting that golden rice cultivars are comparable to conventional rice for grain quality and nutritional traits and safe for human consumption. The drought-tolerant soybean (IND-ØØ41Ø-5) and wheat (IND-ØØ412-7) containing events HaHB4 were compositionally equivalent to their non-transgenic parental controls ( Ayala et al., 2019 ; Chiozza et al., 2020 ). Furthermore, a 42-day broiler feeding trial involving isoenergetic diets containing 40% flour from transgenic wheat and its non-GM counterpart “Cadenza” support the nutritional equivalence of transgenic lines when compared with conventional wheat.…”

Genetically modified organisms: adapting regulatory frameworks for evolving genome editing technologies

“…Soybean and wheat, two of the most consumed crops, have been modified to express sunflower Hahb-4 transcription factor related to water stress responses [ 101 ]. These transgenic crops (termed HB4 crops) are currently commercially available and do not differ in nutritional content compared to their non-transgenic counterparts [ 102 – 104 ]. Under field conditions, HB4 soybean has increased seed yield and water use efficiency in dry environments compared to non-transgenic crops [ 105 ].…”

Three strategies of transgenic manipulation for crop improvement

“…According to the survey carried out by ISAAA (2022), most (227) of the singular transgenic events (265) approved for commercial release worldwide were developed by the first strategy: 210 by bacterial genes (Stalker et al, 1988;Ye et al, 2000;Paine et al, 2005;Castiglioni et al, 2008;Napier et al, 2019), 8 by exogenous genes from mold, algae, fungus, and yeast (Knutzon et al, 1998;Napier et al, 2019;Kinney et al, 2022), 5 by exogenous genes from sexually incompatible plant species (Song et al, 2003;Takagi et al, 2005;Preuss et al, 2012;Rice et al, 2014), and 2 by the mutant copies of the endogenous genes for enhancing herbicide tolerance (McNaughton et al, 2008;EFSA Panel on Genetically Modified Organisms [GMO]et al, 2018;Karthik et al, 2020), respectively (Table 1). Thirty-eight events were developed by the third strategy and transformed by synthetic sequences transcribing antisense or double-stranded RNAs for suppressed expression of undesirable endogenous genes of pathogens, pest insects, and recipient crops themselves (Chen et al, 2003;Davis and Ying, 2004;Tennant et al, 2005;Otani et al, 2007;Ilardi and Nicola-Negri, 2011;Aragao et al, 2013;Ramaseshadri et al, 2013;Carvalho et al, 2015;Orbegozo et al, 2016;Borah et al, 2018;Wu et al, 2018;Callahan et al, 2019;Chiozza et al, 2020). Only one event was transformed by endogenous genes for restoring male fertility (Unger et al, 2002) and another event for pyramiding herbicide tolerance.…”

“…Similar to drought tolerance, the vast majority of the transgenic events overexpressing endogenous disease-resistant genes or homologous disease-resistant genes from sexually incompatible species remain at the testing stage (Anand et al, 2003;Zhao et al, 2005;Yang et al, 2008;Zhou et al, 2009). Twenty-five of the 29 approved events have been developed by the third strategy and transformed with synthetic DNA sequences to transcribe antisense or double-stranded RNAs for the interference of disease viruses (Chen et al, 2003;Davis and Ying, 2004;Tennant et al, 2005;Aragao et al, 2013;Carvalho et al, 2015; 10.3389/fpls.2022.948518 Borah et al, 2018;Wu et al, 2018;Callahan et al, 2019;Chiozza et al, 2020), and only the other four potato events are transformed with exogenous genes of pathogenesis-related proteins from distant species of the nightshade family (Solanum bulbocastanum and Solanum venturii) (Table 1; Halterman et al, 2008;Foster et al, 2009). RNA interference (RNAi) triggered by antisense or double-stranded RNAs described by transformed synthetic DNA sequences is a versatile, effective, safe, and ecofriendly technology for crop protection against viruses and other pathogens as well as insect pests, and delaying maturation of fruits with positive economic, environmental, and human health implications (Klee, 1993;Taning et al, 2020;Giudice et al, 2021;Hernández-Soto and Chacón-Cerdas, 2021).…”