Compound 48/80, a Mast Cell Stimulator, Enhances Synthesis of IgE and IgG Induced by Intranasal Application of Ovalbumin in Mice

Matsui, Nobuaki; Ito, Daisuke; Takabatake, Yukari; Nashioka, Eri; Tada, Shingo; Kanagawa, Masato; Fukuishi, Nobuyuki; Akagi, Masaaki

doi:10.1248/bpb.b15-00637

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…As a mixed polymer of p -methoxy- N -methyl phenylethylamine, the C48/80 compound is widely used to stimulate the release of histamine and TNF-α ( Matsui et al ., 2015 ). Ovalbumin and soy protein are also reported to induce an allergic reaction ( Cordle, 2004 ; Zhang et al ., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Hydrolysis by Alcalase Improves Hypoallergenic Properties of Goat Milk Protein

Jung¹,

Yun²,

Lee³

et al. 2016

Korean Journal for Food Science of Animal Resources

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Goat milk is highly nutritious and is consumed in many countries, but the development of functional foods from goat milk has been slow compared to that for other types of milk. The aim of this study was to develop a goat milk protein hydrolysate (GMPH) with enhanced digestibility and better hypoallergenic properties in comparison with other protein sources such as ovalbumin and soy protein. Goat milk protein was digested with four commercial food-grade proteases (separately) under various conditions to achieve the best hydrolysis of αs -casein and β-lactoglobulin. It was shown that treatment with alcalase (0.4%, 60℃ for 30 min) effectively degraded these two proteins, as determined by SDS-PAGE, measurement of nonprotein nitrogen content, and reverse-phase high-performance liquid chromatography. Hydrolysis with alcalase resulted in a significant decrease in β-lactoglobulin concentration (almost to nil) and a ~40% reduction in the level of αs-casein. Quantification of histamine and TNF-α released from HMC-1 cells (human mast cell line) showed that the GMPH did not induce an allergic response when compared to the control. Hence, the GMPH may be useful for development of novel foods for infants, the elderly, and convalescent patients, to replace cow milk.

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Section: Discussionmentioning

confidence: 99%

Hydrolysis by Alcalase Improves Hypoallergenic Properties of Goat Milk Protein

Jung¹,

Yun²,

Lee³

et al. 2016

Korean Journal for Food Science of Animal Resources

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“…In addition, the incorporation of c48/80 into chitosan nanoparticles as a delivery system has been demonstrated to further enhance mucosal immunity [133,135,137]. C48/80 can also efficiently enhance the synthesis of IgE and IgG upon the intranasal application of ovalbumin [138]. In contrast, c48/80 displayed no adjuvant activity for immunization with UV-attenuated Toxoplasma gondii [139].…”

Section: The Adjuvant Effect Of MC Activators In Vaccinationmentioning

confidence: 99%

Mast Cell Functions Linking Innate Sensing to Adaptive Immunity

Katsoulis‐Dimitriou

Kotrba

Voß

et al. 2020

Cells

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Although mast cells (MCs) are known as key drivers of type I allergic reactions, there is increasing evidence for their critical role in host defense. MCs not only play an important role in initiating innate immune responses, but also influence the onset, kinetics, and amplitude of the adaptive arm of immunity or fine-tune the mode of the adaptive reaction. Intriguingly, MCs have been shown to affect T-cell activation by direct interaction or indirectly, by modifying the properties of antigen-presenting cells, and can even modulate lymph node-borne adaptive responses remotely from the periphery. In this review, we provide a summary of recent findings that explain how MCs act as a link between the innate and adaptive immunity, all the way from sensing inflammatory insult to orchestrating the final outcome of the immune response.

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“…Most often, these studies use cholera toxin (CT) as a control since both the B and A1 subunits are commonly used and effective adjuvants in laboratory experiments [115,116]. One of the most widely used mast cell activators, c48/80, improved generation of neutralizing antibodies over antigen alone and to a comparable level as CT [96,98,99,[101][102][103][104][105]. Antigens included bacterial toxins, bacterial vaccine strains and viral surface antigens.…”

Section: Mast Cell Activators As Vaccine Adjuvantsmentioning

confidence: 99%

“…The potential safety of these adjuvants has been discussed, since they could potentially cause anaphylaxis [27]. With the exception of one study [103], most studies find little if any production of IgE when using mast cell activators as adjuvants [96,98,114]. Yoshino et al also report no adverse effects on the olfactory bulb or kidney when using polymyxins as adjuvants [113].…”

Section: Mast Cell Activators As Vaccine Adjuvantsmentioning

confidence: 99%

Harnessing the Power of Mast Cells in Unconventional Immunotherapy Strategies and Vaccine Adjuvants

Willows

Kulka

2020

Preprint

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Mast cells are long-lived, granular, myeloid-derived leukocytes that have significant protective and repair functions in tissues. Mast cells sense disruptions in the local microenvironment and are first responders to physical, chemical and biological insults. When activated, mast cells release growth factors, proteases, chemotactic proteins and cytokines thereby mobilizing and amplifying the innate and adaptive immune system. Mast cells are therefore significant regulators of homeostatic functions and may be essential in microenvironmental changes during pathogen invasion and disease. During infection by helminths, bacteria and viruses, mast cells release antimicrobial factors to facilitate pathogen expulsion and eradication. Mast cell-derived proteases and growth factors protect tissues from insect/snake bites and exposure to ultraviolet radiation. Finally, mast cells release mediators that promote wound healing in the inflammatory, proliferative and remodeling stages. Since mast cells have such a powerful repertoire of functions, targeting mast cells may be an effective new strategy for immunotherapy of disease and design of novel vaccine adjuvants. In this review, we will examine how certain strategies that specifically target and activate mast cells can be used to treat and resolve infections, augment vaccines and heal wounds. Although these strategies may be protective in certain circumstances, mast cells activation may be deleterious if not carefully controlled and any therapeutic strategy using mast cell activators must be carefully explored.

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Compound 48/80, a Mast Cell Stimulator, Enhances Synthesis of IgE and IgG Induced by Intranasal Application of Ovalbumin in Mice

Cited by 8 publications

References 15 publications

Hydrolysis by Alcalase Improves Hypoallergenic Properties of Goat Milk Protein

Hydrolysis by Alcalase Improves Hypoallergenic Properties of Goat Milk Protein

Mast Cell Functions Linking Innate Sensing to Adaptive Immunity

Harnessing the Power of Mast Cells in Unconventional Immunotherapy Strategies and Vaccine Adjuvants

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