Compound Lactobacillus sp. administration ameliorates stress and body growth through gut microbiota optimization on weaning piglets

Yang, Jiajun; Wang, Chonglong; Huang, Kehe; Zhang, Min-Hong; Wang, Jing; Pan, Xiaocheng

doi:10.1007/s00253-020-10727-4

Cited by 42 publications

(36 citation statements)

References 46 publications

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“…Up to now, the few studies that have investigated the effect of L. salivarius on human intestinal microbiota (Drago et al, 2012;Larsen et al, 2013) have been performed on faecal (and not on colonic) samples and have followed only specific bacterial populations, making difficult any comparison with our study. Of note, a very recent study performed by Yang et al (2020) showed that a mix of L. salivarius and L. reuteri strains induced both a decrease in Bacteroidetes and an increase in Ruminococcaceae relative abundances in piglet caecal microbiota, which is in line with our results. By using mutants, Riboulet-Bisson et al (2012) showed that the effect of L. salivarius on gut microbiota seems to be, at least partially, bacteriocin-dependant.…”

Section: Beneficial Microbessupporting

confidence: 93%

Impact of oral galenic formulations of Lactobacillus salivarius on probiotic survival and interactions with microbiota in human in vitro gut models

Arnal

Denis

Uriot

et al. 2021

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Health benefits of probiotics in humans essentially depend on their ability to survive during gastrointestinal (GI) transit and to modulate gut microbiota. To date, there is few data on the impact of galenic formulations of probiotics on these parameters. Even if clinical studies remain the gold standard to evaluate the efficacy of galenic forms, they stay hampered by technical, ethical and cost reasons. As an alternative approach, we used two complementary in vitro models of the human gut, the TNO gastrointestinal (TIM-1) model and the Artificial Colon (ARCOL), to study the effect of three oral formulations of a Lactobacillus salivarius strain (powder, capsule and sustained-release tablet) on its viability and interactions with gut microbiota. In the TIM-1 stomach, no or low numbers of bacteria were respectively released from the capsule and tablet, confirming their gastro-resistance. The capsule was disintegrated in the jejunum on average 76 min after administration while the core of sustained-release tablet was still intact at the end of digestion. Viability in TIM-1 was significantly influenced by the galenic form with survival percentages of 0.003±0.004%, 2.8±0.6% and 17.0±1.8% (n=3) for powder, capsule and tablet, respectively. In the ARCOL, the survival of the strain tended to be higher in the post-treatment phase with the tablet compared to capsule, but gut microbiota composition and activity were not differently modulated by the two formulations. In conclusion, the sustained-release tablet emerged as the formulation that most effectively preserved viability of the tested strain during GI passage. This study highlights the usefulness of in vitro gut models for the pre-screening of probiotic pharmaceutical forms. Their use could also easily be extended to the evaluation of the effects of food matrices and age on probiotic survival and activity during GI transit.

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Section: Beneficial Microbessupporting

confidence: 93%

Impact of oral galenic formulations of Lactobacillus salivarius on probiotic survival and interactions with microbiota in human in vitro gut models

Arnal

Denis

Uriot

et al. 2021

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“…and Lactobacillus), decrease E. coli, and enhance the immunity and antioxidant capacity in the colon and plasma of offspring weaned piglets. Dietary probiotics or synbiotics supplementation is usually considered an effective way to positively modulate the intestinal microbiota in weaned piglets (9,23). Based on the colonic microbiota analyses, we found that dietary probiotics or synbiotics supplementation significantly increase the relative abundances of Firmicutes and Bacteroidetes.…”

Section: Discussionmentioning

confidence: 74%

“…Probiotics refer to live microorganisms, which are administered in adequate amounts and confer microbial balance, particularly in the gastrointestinal tract (7). Several studies demonstrated that the provision of probiotics could optimize the intestinal microbiota composition, present significant antioxidant abilities, and improve immune function in weaned piglets (8)(9)(10). When xylo-oligosaccharide (XOS) are used together with probiotics, they are called synbiotics, which can reconstruct intestinal microbiota by promoting the proliferation of probiotics bacteria (11).…”

Section: Introductionmentioning

confidence: 99%

Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets

et al. 2020

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This study was conducted to investigate the effect of dietary probiotics or synbiotics supplementation on colonic microbiota, antioxidant capacity, and immune function in weaned piglets. A total of 64 pregnant Bama mini-sows and then 128 of their weaned piglets were randomly assigned into control group, antibiotics group, probiotics group, or synbiotics group. The results showed that colonic Firmicutes and Bifidobacterium abundances in the probiotics group and total bacteria, Bacteroidetes, and Lactobacillus abundances in the synbiotics group were increased (P < 0.05), while Escherichia coli abundance in the synbiotics group was decreased (P = 0.061) compared with the control group. Firmicutes, Bifidobacterium, and total bacteria abundances were increased (P < 0.05) in the probiotics and synbiotics groups compared with the antibiotics group. Probiotics supplementation up-regulated (P < 0.05) the mRNA expression of GPR109A compared with the control and antibiotics groups. Dietary probiotics or synbiotics supplementation improved the antioxidant capacity by increasing (P < 0.05) the colonic CAT, GSH-Px, SOD, and T-AOC levels and plasma CAT, GSH, GSH-Px, and SOD levels and by decreasing (P < 0.05) the colonic and plasma MDA and H2O2 levels. Compared to the control group, the colonic IL-10, IFN-α, and sIgA concentrations and plasma IgA and IgM concentrations were significantly increased (P < 0.05) in the probiotics and synbiotics groups. Spearman's correlation analysis showed that the changed colonic microbiota, such as Lactobacillus and Bifidobacterium were correlated with the alteration of antioxidant indexes, cytokines, and immunoglobulins. In conclusion, dietary probiotics or synbiotics supplementation during gestation, lactation, and nursery periods could be used as an alternative for antibiotics in terms of gut health of weaned piglets.

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“…The primers for amplifying the V3–V4 hypervariable regions of the bacterial 16S rRNA genes were 338F-ACTCCTACGGGAGGCAGCAG and 806R-GGACTACHVGGGTWTCTAAT and an ABI GeneAmp ® 9700 PCR thermocycler (ABI, CA, USA) was used ( 31 ). The PCR amplification system and conditions have been previously described ( 32 ). The PCR products were extracted from 2% agarose gels and purified using AxyPrep DNA gel extraction kits (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA was extracted from the microbial community in cecal digestive samples using QIAamp Fast DNA Stool mini kits (Qiagen, Hilden, Germany) following the manufacturer's (31). The PCR amplification system and conditions have been previously described (32). The PCR products were extracted from 2% agarose gels and purified using AxyPrep DNA gel extraction kits (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus TM fluorometer (Promega, USA).…”

Section: Dna Extraction Polymerase Chain Reaction Amplification and Sequencingmentioning

confidence: 99%

Effects of Combined Supplementation of Macleaya cordata Extract and Benzoic Acid on the Growth Performance, Immune Responses, Antioxidant Capacity, Intestinal Morphology, and Microbial Composition in Weaned Piglets

et al. 2021

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Because the use of antibiotics is forbidden, piglets experience a considerable weanling stress, resulting in increased incidence of diarrhea and death. Macleaya cordata extract or benzoic acid have anti-inflammatory, antioxidant, and antimicrobial activities that makes them potential antibiotic alternatives. The objective of this study was to evaluate the potential effects of feed supplemented with Macleaya cordata extract and benzoic acid on growth performance, immunity, antioxidant capacity, intestinal morphology, and microflora in weaned piglets. Twenty-four weaned piglets [Duroc × (Large White × Landrace)] 28 days of age and weighing 8.41 ± 0.13 kg were randomly divided in equal numbers (n = 8) into three groups fed a basal diet (CON), CON + 20 mg/kg flavomycin + 50 mg/kg quinocetone (AGP), or CON + 50 mg/kg Macleaya cordata extract + 1,000 mg/kg benzoic acid (MB). Compared with the CON diet, dietary MB or AGP increased the final weight and average daily gain, and reduced feed efficiency and the diarrhea rate (P < 0.05). Compared with the CON diet, MB supplementation increased serum superoxide dismutase (SOD activity) and decreased malondialdehyde (MDA) content (P < 0.05). Serum interleukin (IL)-10 IgA and IgM were higher (P < 0.05) in MB-fed piglets than in CON-fed piglets. Piglets fed the MB diet had greater villus height and villus height to crypt depth ratio (VC) in the duodenum, villus height in the ileum, and lower crypt depth in the jejunum than did piglets given the CON diet (P < 0.5). Piglets in the MB group had increased concentrations of acetate, propionate, butyrate, and total short-chain fatty acids in the ileum or cecum compared with the CON and AGP groups (P < 0.05). Streptococcus proportion was lower in the MB than in the AGP group. Dietary MB increased the Lactobacillus and decreased Escherichia-Shigella populations compared with the CON group (P < 0.05). The study results indicate that MB can be used to replace AGP as a feed supplement for weaned piglets.

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Compound Lactobacillus sp. administration ameliorates stress and body growth through gut microbiota optimization on weaning piglets

Cited by 42 publications

References 46 publications

Impact of oral galenic formulations of Lactobacillus salivarius on probiotic survival and interactions with microbiota in human in vitro gut models

Impact of oral galenic formulations of Lactobacillus salivarius on probiotic survival and interactions with microbiota in human in vitro gut models

Dietary Probiotics or Synbiotics Supplementation During Gestation, Lactation, and Nursery Periods Modifies Colonic Microbiota, Antioxidant Capacity, and Immune Function in Weaned Piglets

Effects of Combined Supplementation of Macleaya cordata Extract and Benzoic Acid on the Growth Performance, Immune Responses, Antioxidant Capacity, Intestinal Morphology, and Microbial Composition in Weaned Piglets

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