Comprehensive Analysis of CRISPR-Cas9 Editing Outcomes in Yeast Xanthophyllomyces dendrorhous

The biological principles and advanced applications of DSB repair in CRISPR-mediated yeast genome editing

“…lipolytica – – AtoB, HMGR, HMGS α-farnesene production [ 80 ] Y . lipolytica CRISPR–Cas9, gRNA Random integration: 1.6 × 10 4 colonies/μg DNA Leu2 Enhance production of lipase and β‐carotene [ 81 ] Xanthophyllomyces dendrorhous CRISPR–Cas9, gRNA 64.3% of point mutants, 23.8 of deletion, 4.8% of chromosome rearrangement CrtE and CrtS – [ 89 , 90 ] …”

“…Although NHEJ efficiency in some yeast species is relatively low, applying the CRISPR system and NHEJ for genome editing in these yeast species could still be achieved. Researchers have achieved Cas9 cleavage in the yeast Xanthophyllomyces dendrorhous with less NHEJ dominance, along with performing Sanger/Illumina sequencing to identify diverse DNA repair patterns, including DNA insertions/deletions, intrachromosomal translocations, and on-target nucleotide substitutions (point mutations) [ 89 ]. Two genes, CrtE and CrtS, encoding the color of X .…”