Comprehensive Analysis of Gut Microbiota and Fecal Bile Acid Profiles in Children With Biliary Atresia

Yang, Ting; Shen, Yang; Wang, Peize; Li, Siqi; Jin, Yuyan; Liu, Zhaozhou; Zhang, Xinyue; Zhang, Yanan; Zhao, Yong; Liao, Junmin; Li, Shuangshuang; Hua, Kaiyun; Gu, Yichao; Wang, Dingding; Huang, Jinshi

doi:10.3389/fcimb.2022.914247

Cited by 16 publications

(12 citation statements)

References 59 publications

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“…Recently, the role of intestinal flora in BA has received attention. A disturbed gut microbiota structure was identified in the BA group [ 52 ] and correlated with the occurrence of postoperative cholangitis and jaundice clearance [ 53 , 54 ]. Wang et al conducted 16S ribosomal RNA gene sequencing on the intestinal flora of 16 BA patients after surgery in 2020, and the results showed that the intestinal flora diversity of BA patients decreased and the overall structure changed, among which the proportion of Clostridium decreased significantly [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Biliatresone: progress in biliary atresia study

et al. 2022

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Background Biliary atresia (BA) is one of the main causes of neonatal end-stage liver disease. Without timely diagnosis and treatment, most children with BA will develop irreversible liver fibrosis within the first two months. While current theorized causes of BA include viral infection, immune disorders, and genetic defects, the comprehensive etiology is still largely unknown. Recently, biliatresone attracted much interest for its ability to induce BA in both zebrafish and mice, so we summarized the latest progress of biliatresone research in BA and tried to answer the question of whether it could provide further clues to the etiology of human BA. Data sources We conducted a PubMed search for any published articles related to the topic using search terms including “biliary atresia”, “biliatresone”, “GSH”, and “HSP90”. Relevant data were extracted from the original text or supplementary materials of the corresponding articles. Results Biliatresone had shown its unique toxicity in multiple species such as zebrafish and mice, and pathogenic factors involved included glutathione (GSH), heat shock protein 90 (HSP90) and the related pathways. In combination with epidemiological evidence and recent studies on the intestinal flora in biliary atresia, a new pathogenic hypothesis that the occurrence of biliary atresia is partly due to biliatresone or its structure-like compounds depositing in human body via vegetables or/and the altered intestinal flora structure can be tentatively established. Conclusions Based on the existing evidence, we emphasized that GSH and HSP90 are involved in the development of BA, and the maternal diet, especially higher vegetable intake of Asian women of childbearing age, accompanied by the altered intestinal flora structure, may contribute to the occurrence of biliary atresia and the higher incidence in the Asia group. However, the evidence from large sample epidemiological research is necessary.

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Section: Introductionmentioning

confidence: 99%

Biliatresone: progress in biliary atresia study

et al. 2022

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“…Colidextribacter was significantly positively correlated with pro-inflammatory factors, negatively correlated with antioxidant enzyme activities and anti-inflammatory factors [ 51 ], and significantly reduced the abundance of Colidextribacter in the colon in weaned piglet diets supplemented with probiotics Clostridium butyricum [ 18 ]. In addition, there are few reports on norank_f__Selenomonadaceae , which belongs to the family level Selenomonadaceae and is enriched in patients with episodes of cholangitis [ 52 ]. Correlation analysis also showed that norank_f__Selenomonadaceae and Colidextribacter were significantly negatively correlated with serum immune indexes (IgG, IgM), antioxidant index T-AOC, and intestinal-barrier-related indexes (ZO-1, Occludin).…”

Section: Discussionmentioning

confidence: 99%

Dietary Fiber-Derived Butyrate Alleviates Piglet Weaning Stress by Modulating the TLR4/MyD88/NF-κB Pathway

Huangfu,

Ma,

Zhang

et al. 2024

Nutrients

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During weaning, piglets are susceptible to intestinal inflammation and impairment in barrier function. Dietary fiber (DF) plays an active role in alleviating weaning stress in piglets. However, the effects of different sources of dietary fiber on the performance of weaned piglets are inconsistent, and the mechanisms through which they affect intestinal health need to be explored. Therefore, in this study, sixty weaned piglets were randomly divided into three treatment groups: basal diet (control, CON), beet pulp (BP), and alfalfa meal (AM) according to the feed formulation for a 28-day trial. The results showed that both AM and BP groups significantly reduced diarrhea rate and serum inflammatory factors (IL-1β and TNF-α) and increased antioxidant markers (T-AOC and SOD), in addition to decreasing serum MDA and ROS concentrations in the AM group. At the same time, piglets in the AM group showed a significant reduction in serum intestinal permeability indices (LPS and DAO) and a substantial increase in serum immunoglobulin levels (IgA, IgG, and IgM) and expression of intestinal barrier-associated genes (Claudin1, Occludin, ZO-1, and MUC1), which resulted in an improved growth performance. Interestingly, the effect of DF on intestinal inflammation and barrier function can be attributed to its modulation of gut microbes. Fiber-degrading bacteria enriched in the AM group (Christensenellaceae_R-7_group, Pediococcus and Weissella) inhibited the production of TLR4- through the promotion of SCFAs (especially butyrate). MyD88-NF-κB signaling pathway activation reduces intestinal inflammation and repairs intestinal barrier function. In conclusion, it may provide some theoretical support and rationale for AM to alleviate weaning stress and improve early intestinal dysfunction, which may have implications for human infants.

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“…[ 3 ] Blautia is a new functional genus with putative probiotic properties. [ 49 ] Moreover, as the dominant genus of bacteria in kefir‐treated mice, Parasutterella has impacted BA transport and synthesis and thereby participated in the maintenance of BA homeostasis. [ 50 ] In conclusion, bacterial alterations in alcohol‐related diseases are largely characterized by reductions in beneficial communities and increases in the pathogenic taxa.…”

Section: Discussionmentioning

confidence: 99%

Kefir Ameliorates Alcohol‐Induced Liver Injury Through Modulating Gut Microbiota and Fecal Bile Acid Profile in Mice

Cui,

Jing,

Yue

et al. 2023

Molecular Nutrition Food Res

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ScopeAlcoholic liver disease (ALD) is the leading cause of liver‐related deaths worldwide. Kefir has been studied for its properties of anti‐obesity, rebuilding intestinal homeostasis, and alleviating non‐alcoholic fatty liver disease. However, the possible role of kefir in the prevention or treatment of ALD has not been carefully considered. Here, it evaluated the protective effects of kefir supplementation on alcohol‐induced liver injury.Methods and resultsC57BL/6J mice are fed to Lieber‐DeCarli liquid diet containing alcohol to build ALD mouse model, followed by oral administration with kefir. Results indicate that kefir treatment improves liver pathological changes, decreases the expression levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and inflammatory markers, and increases antioxidant levels. Kefir supplementation also restores the intestinal barrier and altered microbial composition, indicates as increases of Blautia, Bacteroides, and Parasutterella and decreases in the Firmicutes/Bacteroidetes (F/B) ratio and populations of Psychrobacter, Bacillus, and Monoglobus. Moreover, kefir supplementation decreases the levels of total bile acids (BAs) and primary BAs and increases the secondary/primary BA ratio. Gut microbes play a key role in the conversion of primary to secondary fecal BAs.ConclusionKefir can ameliorate ALD through regulating the composition of the gut microbiota.

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Comprehensive Analysis of Gut Microbiota and Fecal Bile Acid Profiles in Children With Biliary Atresia

Cited by 16 publications

References 59 publications

Biliatresone: progress in biliary atresia study

Biliatresone: progress in biliary atresia study

Dietary Fiber-Derived Butyrate Alleviates Piglet Weaning Stress by Modulating the TLR4/MyD88/NF-κB Pathway

Kefir Ameliorates Alcohol‐Induced Liver Injury Through Modulating Gut Microbiota and Fecal Bile Acid Profile in Mice

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