Comprehensive Analysis of MYB Gene Family and Their Expressions Under Abiotic Stresses and Hormone Treatments in Tamarix hispida

Abstract

The MYB transcription factors (TFs) is a plant TF families, which involves in hormone signal transduction, and abiotic stress tolerance, etc. However, there are few studies on the MYB TFs family and its regulatory mechanism in Tamarix hispida. In this study, 14 MYB genes (named ThMYB1 - ThMYB14) were cloned and characterized from T. hispida. The transcription profiles of ThMYBs in T. hispida under different abiotic stress conditions were monitored using qRT-PCR. Most of studied ThMYBs were significantly downregulated and/or upregulated by salt and osmotic stress, ABA, GA3 and JA treatments in at least one organ. Especially, ThMYB13 was induced in the leaves and roots of T. hispida when exposed to NaCl treatment at all study periods, indicating that it may involve in salt stress. To further study ThMYB13 function, ThMYB13 overexpression and knock-down plants and control plants transformed with an empty pROKII were obtained using a transient transformation system. Overexpression of ThMYB13 in T. hispida displayed the lowest O2-, H2O2 and MDA accumulation, minimal cell death, the most stable K+/Na+ ratio and the lowest electrolyte leakage rate among the three kinds of transient expression in T. hispida. Conversely, the RNAi-silencing, transiently transformed plants displayed the opposite physiological changes. Therefore, ThMYB13 might play a role in salt stress tolerance in transgenic T. hispida plants.

Meta-Analysis of Expression of the Stress Tolerance Associated Genes and Uncover their Cis-Regulatory Elements in Rice (Oryza sativa L.)

“…Heat shock element is a transcription factor that binds to HSE cis-acting elements in the promoter of stressinducible genes and plays central roles in the acquisition of plant tolerance against abiotic stresses [101]. MBS, which is a binding site for MYB transcription factors, controls many abiotic stress responses [102,103]. Molecular approaches discerned the functional characterization of MYB domain proteins, particularly the R2R3-type members in various plant species, including rice, maize and soybean [103,104].…”

“…MBS, which is a binding site for MYB transcription factors, controls many abiotic stress responses [102,103]. Molecular approaches discerned the functional characterization of MYB domain proteins, particularly the R2R3-type members in various plant species, including rice, maize and soybean [103,104]. A genome-wide comparative analysis of MYB genes and their expression in arabidopsis and rice suggested the potential role of MYB domain proteins in plant stress responses [105].…”

Screening and identification of miRNAs related to sexual differentiation of strobili in Ginkgo biloba by integration analysis of small RNA, mRNA, and degradome sequencing

“…MYB is a large family of transcription factors in plants and involved in many life activities such as plant secondary metabolism [42], hormone responses [43], leaf organ formation [44], ower development [45].…”

Overexpression of ThSOS from Tamarix hispida improves salt tolerance

“…The transient transformation of T. hispida plants was performed according to Zhang [24]. Three groups of transgenic plants were generated by transient transformation with 35S::ThSOS3 of overexpress ThSOS3 gene (OE), with pFGC::ThSOS3 for silencing ThSOS3 (SE) expression, or the empty pROK2 plasmid as control (Con).…”