Comprehensive Analysis of Prognostic and immune infiltrates for FOXPs Transcription Factors in Human Breast Cancer

Yi, Jianing; Tan, Siyi; Zeng, Yuanjun; Zou, Lianhong; Zeng, Jie; Zhang, Chaojie; Liu, Luyao; Fan, Peizhi

doi:10.1038/s41598-022-12954-3

Cited by 6 publications

(11 citation statements)

References 56 publications

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“…NAT10/ac4C/FOXP1 axis was reported to facilitate immunosuppression and overexpression of FOXP1 was found to be positively related to the infiltration of activated CD4+ T cells and Tregs in cervical cancer, 33 which supports our hypothesis. In breast cancer, the expression of FOXP1 is positively correlated with the infiltration of CD4 + T cells, CD8 + T cells, neutrophils and macrophages, but different from our findings, the expression of FOXP1 is negatively correlated with B cells 52 . We speculated that FOXP1 may regulate different immune infiltrating cells depending on different tumours.…”

Section: Discussioncontrasting

confidence: 99%

An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

Ye,

Pan,

Zhu

et al. 2024

J Cellular Molecular Medi

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Forkhead box protein 1 (FOXP1) serves as a tumour promoter or suppressor depending on different cancers, but its effect in oesophageal squamous cell carcinoma has not been fully elucidated. This study investigated the role of FOXP1 in oesophageal squamous cell carcinoma through bioinformatics analysis and experimental verification. We determined through public databases that FOXP1 expresses low in oesophageal squamous cell carcinoma compared with normal tissues, while high expression of FOXP1 indicates a better prognosis. We identified potential target genes regulated by FOXP1, and explored the potential biological processes and signalling pathways involved in FOXP1 in oesophageal squamous cell carcinoma through GO and KEGG enrichment, gene co‐expression analysis, and protein interaction network construction. We also analysed the correlation between FOXP1 and tumour immune infiltration levels. We further validated the inhibitory effect of FOXP1 on the proliferation of oesophageal squamous cell carcinoma cells through CCK‐8, colony formation and subcutaneous tumour formation assays. This study revealed the anticarcinogenic effect of FOXP1 in oesophageal squamous cell carcinoma, which may serve as a novel biological target for the treatment of tumour.

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Section: Discussioncontrasting

confidence: 99%

An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

Ye,

Pan,

Zhu

et al. 2024

J Cellular Molecular Medi

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“…But the prognostic value of FOXP3 in cancer appears to be divergent. As a transcription factor, FOXP3 may play a regulatory role by directly binding to specific tumor-related genes in BC (9). This ability of transcriptional regulation may well be the potential superiority of FOXP3 in relative to prior biomarkers of BC.…”

Section: Introductionmentioning

confidence: 99%

The expression landscape of FOXP3 and its prognostic value in breast cancer

Li¹,

Zhang²,

Liu³

et al. 2022

Ann Transl Med

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Background: Forkhead Box Protein 3 (FOXP3), as an essential marker of regulatory T cell (Treg) development, is reportedly overexpressed in invasive breast carcinoma (BRCA) and could be a potential prognostic factor for BRCA. However, the biological function of FOXP3 in BRCA is still unclear. In this study, we comprehensively explored the expression landscape of FOXP3 and its prognostic value in BRCA.Methods: FOXP3 transcriptomic expression data were mainly obtained from The Cancer Genome Atlas (TCGA). The Kaplan-Meier plotter and receiver operating characteristic (ROC) curve were used to assess the prognostic and diagnostic value of FOXP3 in BRCA. UALCAN, cBio-Portal, and MethSurv were used to evaluate the genomic variation of FOXP3. Gene set enrichment analysis (GSEA) was performed to explore the FOXP3 pathways involved in BRCA. Morover, we detected the expression of FOXP3 in 123 BRCA specimens and 5 BRCA cell lines to verify the biological value of FOXP3 in BRCA. The Kaplan-Meier method was adopted for the overall survival (OS) analysis, and a Cox proportional hazards model was used to estimate the hazard ratio (HR) for OS.Results: FOXP3 was more highly expressed in BRCA than in normal tissues (2.808±1.020 vs. 1.409±0.656, P<0.001), and overexpressed FOXP3 was associated with a better prognosis. The ROC curve demonstrated a significant diagnostic value of FOXP3 in BRCA (area under the ROC curve, AUC: 0.877). Genomic analysis revealed that promoter hypomethylation of FOXP3 may be the underlying mechanism of FOXP3's upregulation in BRCA. GSEA found that FOXP3 coexpressed genes were mainly involved in the Toll-like receptor pathway, JAK/STAT pathway, cell cycle, and apoptosis. Moreover, high FOXP3 expression was an independent protective factor for OS in our 123 BRCA tissues (HR: 0.367; P=0.036). In vitro, we found that FOXP3 knockdown with siRNA promoted migration and invasion in MCF-7 cells.Conclusions: This study demonstrated that FOXP3 shows prognostic and diagnostic value for BRCA.We provided evidence that promoter hypomethylation and a high expression of FOXP3 were both related to a favorable prognosis in BRCA, which maybe associated with the Toll-like receptor pathway, JAK/STAT pathway, cell cycle, and apoptosis.

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“…4 The previous studies have showed that FOXPs and GPI were prognostic biomarkers for breast cancer. 5,6 However, these biomarkers were not suitable for all breast cancer patients, owing to tumor heterogeneity and individual differences. 7 Therefore, to improve patient survival, identifying additional suitable prognostic biomarkers is essential to enhance the precision of clinical outcome predictions, tailor personalized treatment strategies more effectively, and innovate new therapeutic approaches for breast cancer.…”

Section: Introductionmentioning

confidence: 99%

RPGRIP1L as a new biomarker for prognosis and tumor immune of breast cancer

Yi,

Liu,

Chen

et al. 2024

The FASEB Journal

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The Retinitis pigmentosa GTPase regulator interacting protein 1‐like (RPGRIP1L) gene encodes an important protein that performs various physiological functions. Variants of RPGRIP1L are related to a number of diseases. However, it is currently unknown whether RPGRIP1L is correlated with breast invasive carcinoma (BRCA). In BRCA tissue specimens, the expression of RPGRIP1L was found to be elevated in comparison to its levels in normal breast tissue. A notable decline in survival rates was associated with patients exhibiting heightened RPGRIP1L gene expression. Consistent with these findings, our data also show the above results. Furthermore, elevated expression of RPGRIP1L corresponded with a spectrum of unfavorable clinicopathological features, including the presence of human epidermal growth factor receptor 2 (HER2) positive, estrogen receptor (ER) positive, over 60 years old, T2, N0, and N3. At the same time, our research indicated that 50 genes and 15 proteins were positively related to RPGRIP1L, and that these proteins and genes were mostly involved in T cell proliferation, immune response, cytokine activity, and metabolic regulation. In addition, in the present study, there was a significant correlation between RPGRIP1L expression and immune cell infiltration. Finally, we found that four Chemicals could downregulate the expression of RPGRIP1L. Altogether, our results strongly indicated that RPGRIP1L might serve as a new prognostic biomarker for BRCA.

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Comprehensive Analysis of Prognostic and immune infiltrates for FOXPs Transcription Factors in Human Breast Cancer

Cited by 6 publications

References 56 publications

An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

The expression landscape of FOXP3 and its prognostic value in breast cancer

RPGRIP1L as a new biomarker for prognosis and tumor immune of breast cancer

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