Comprehensive Analysis of the Expression Profiles of Hepatic lncRNAs in the Mouse Model of Alcoholic Liver Disease

Abstract

Background and Aim: The worldwide prevalence of alcoholic liver disease (ALD) due to escalating alcohol consumption has presented an unprecedented pressure on human health. A few studies have determined long non-coding RNAs (lncRNAs) involved in the pathogenesis of liver diseases. However, the roles of lncRNAs in ALD development is still poorly understood.Methods: An ALD mouse model was established and confirmed. Expression profiles of lncRNAs were obtained by whole transcriptome sequencing. The altered lncRNAs in ALD mice were further verified by qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to enrich the functions of these lncRNAs. In combination with miRNA and mRNA profiles, we constructed concise endogenous RNA (ceRNA) networks. The function of the most up/downregulated lnRNA was further verified and investigated in both ALD model and AML-12 cells.Results: Totally, five downregulated lncRNAs were obtained and verified in ALD mice. The GO term and KEGG pathway analyses revealed that the identified lncRNAs were associated with alcohol-induced hepatic oxidative damage, cellular inflammation, and lipid metabolism. Combination the differentially modulated miRNAs and mRNAs with ceRNA network analysis, we constructed five ceRNA networks and obtained 30 miRNAs and 25 mRNAs that may participate in ALD. Further, we verified and investigate the function of the most downregulated lnc_1700023H06Rik. Depletion lnc_1700023H06Rik reduced genes encoding for lipid metabolism, especially mRNA Acat2 (ENSMUST00000159697) and Pgrmc2 (ENSMUST00000058578) both in vivo and in vitro. Knocking down lnc_1700023H06Rik induced triglyceride accumulation and lactate dehydrogenase leakage in AML12 cells, consisting with that in alcohol-treated cells.Conclusion: The five remarkably downregulated lncRNAs in ALD mouse model were identified as novel biomarkers, highlighting the key role of lncRNAs in the development of ALD. The effect of lnc_1700023H06Rik plays a pivotal role in lipid deposition and its pathological pathway in ALD needs further investigation.

Regulatory Networks of Prognostic mRNAs in Pediatric Acute Myeloid Leukemia

“…The miRNA and lcRNA are a large number of functional noncoding RNAs in the body, which can regulate transcription. The miRNA and lcRNA have specific structures and biological functions and participate in various physiological and pathological processes of the body [ 7 – 12 ]. At present, studies have shown that the role of the lncRNA and miRNA in tumors has been initially recognized, and some studies have shown that the lncRNA and miRNA can be used as biomarkers of AML [ 13 , 14 ].…”

Epigenetics of alcohol-related liver diseases

“…Multiple studies have linked lncRNA to the development of ARLD and progression to HCC. Dou et al [101] analyzed the effect of alcohol on lncRNA expression profiles in a murine ARLD model. In total, 29 lncRNAs were identified, 17 of which were downregulated.…”

“…Furthermore, 5 regulatory networks were constructed to provide a deeper understanding of the mechanism of action of these lncRNAs in ARLD, but validation studies are awaited. [101] Also, through its interaction with SIRT1, the lncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) has J o u r n a l P r e -p r o o f been shown to propagate fibrosis and inflammation. During liver injury, lnc-MALAT1 is overexpressed.…”

“…Dou et al. 100 analysed the effect of alcohol on lncRNA expression profiles in a murine ARLD model. In total, 29 lncRNAs were identified, 17 of which were downregulated.…”

Editorial: Alcohol Consumption and Liver Diseases: From Pathology to Phytotherapy

“…Ethanol oxidation by alcohol dehydrogenase and aldehyde dehydrogenase leads to a reduction of the NAD + /NADH ratio, and then disturbs fatty acid oxidation, glycolysis and glucogenesis, and the tricarboxylic acid cycle, contributing to hepatic steatosis; meanwhile, ethanol oxidation by the microsomal ethanol oxidizing system causes excessive reactive oxygen species generation, which further attacks biological macromolecules resulting in liver injury ( Ceni et al, 2014 ). Emerging evidence shows that alcohol-mediated modification on intracellular biological macromolecules, such as RNA, DNA, and protein, as well as epigenetic modification, contributes to the development of ALD ( Smathers et al, 2011 ; Chen et al, 2020 ; Dou et al, 2020 ; Dou et al, 2021 ; Zhang et al, 2021 ).…”