Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

Fukushima, Hiroshi; Katsube, K.; Tsunomori, Yoshie; Kishi, Ryoko; Atsuta, Junko; Akiba, Yuko

doi:10.1155/2009/917623

Cited by 18 publications

(14 citation statements)

References 26 publications

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“…A range of PCR assays targeting different genomic fragments have been developed for the detection of P. shigelloides (Table 9) (42,51,221,222,223,224,225). One of the earliest assays was configured by González-Rey et al (42) and targeted a specific variable region of the 23S rRNA gene previously identified by Van Camp et al (226).…”

Section: Laboratory Identification Isolation and Identificationmentioning

confidence: 99%

“…This is an advance on 16S rRNA sequencing, where P. shigelloides previously showed a closer affinity with species of the Enterobacteriaceae than with the Aeromonadaceae (19). The gyrB gene has also been used as a target in a PCR assay to detect P. shigelloides in investigations of foodborne outbreaks (223,224).…”

Section: Laboratory Identification Isolation and Identificationmentioning

confidence: 99%

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Plesiomonas shigelloides Revisited

Janda¹,

2016

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SUMMARYAfter many years in the familyVibrionaceae, the genusPlesiomonas, represented by a single species,P. shigelloides, currently resides in the familyEnterobacteriaceae, although its most appropriate phylogenetic position may yet to be determined. Common environmental reservoirs for plesiomonads include freshwater ecosystems and estuaries and inhabitants of these aquatic environs. Long suspected as being an etiologic agent of bacterial gastroenteritis, convincing evidence supporting this conclusion has accumulated over the past 2 decades in the form of a series of foodborne outbreaks solely or partially attributable toP. shigelloides. The prevalence ofP. shigelloidesenteritis varies considerably, with higher rates reported from Southeast Asia and Africa and lower numbers from North America and Europe. Reasons for these differences may include hygiene conditions, dietary habits, regional occupations, or other unknown factors. Other human illnesses caused byP. shigelloidesinclude septicemia and central nervous system disease, eye infections, and a variety of miscellaneous ailments. For years, recognizable virulence factors potentially associated withP. shigelloidespathogenicity were lacking; however, several good candidates now have been reported, including a cytotoxic hemolysin, iron acquisition systems, and lipopolysaccharide. WhileP. shigelloidesis easy to identify biochemically, it is often overlooked in stool samples due to its smaller colony size or relatively low prevalence in gastrointestinal samples. However, one FDA-approved PCR-based culture-independent diagnostic test system to detect multiple enteropathogens (FilmArray) includesP. shigelloideson its panel. Plesiomonads produce β-lactamases but are typically susceptible to many first-line antimicrobial agents, including quinolones and carbapenems.

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Section: Laboratory Identification Isolation and Identificationmentioning

confidence: 99%

Section: Laboratory Identification Isolation and Identificationmentioning

confidence: 99%

Plesiomonas shigelloides Revisited

Janda¹,

2016

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“…Real-time PCR (qPCR) assays have gained confidence as faster and reliable alternatives (Postollec et al 2011 ), and several qPCR assays have already been developed (e.g. Beutin et al 2009 ; Botteldoorn et al 2003 ; Fukushima et al 2009 ; Garrido et al 2012b ; Kim and Cho 2010 ; Löfström et al 2010 ; Singh et al 2012 ). However, their use all together into the same detection system is hampered by several drawbacks: they are using different technologies (e.g.…”

Section: Introductionmentioning

confidence: 99%

SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels

Barbau-Piednoir

Bertrand

Mahillon

et al. 2013

Appl Microbiol Biotechnol

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In this work, a three-level Salmonella detection system based on a combination of seven SYBR®Green qPCR was developed. This detection system discriminates Salmonella at the genus, species and subspecies levels using a single 96-well plate. The SYBR®Green qPCR assays target the invA, rpoD, iroB and safC genes, as well as the STM0296 locus, putatively coding for a cytoplasmic protein. This study includes the design of primer pairs, in silico and in situ selectivity, sensitivity, repeatability and reproducibility evaluations of the seven SYBR®Green qPCR assays. Each detection level displayed a selectivity of 100 %. This combinatory SYBR®Green qPCR system was also compared with three commercially available Salmonella qPCR detection kits. This comparison highlighted the importance of using a multi-gene detection system to be able to detect every target strain, even those with deletion or mutation of important genes.

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“…7 LNA probes have recently been used to differentiate between the same species (a wild-type and a mutant) with a single base mutation 8 and have also been employed in food-safety-based assays. 7 RT-PCR has been widely used to recognize unwanted materials or contaminants in foods, including pathogens, 9,10 allergens, 11−13 and other unexpected products. 14−16 Designing RT-PCR assays for the identification of closely related species of plants is challenging because there is often little or no differentiation in the gene regions typically used to identify plants.…”

Section: ■ Introductionmentioning

confidence: 99%

Development of a Locked Nucleic Acid Real-Time Polymerase Chain Reaction Assay for the Detection of Pinus armandii in Mixed Species Pine Nut Samples Associated with Dysgeusia

Handy

Timme

Jacob

et al. 2013

J. Agric. Food Chem.

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Recent work has shown that the presence of the species Pinus armandii , even when occurring as species mixtures of pine nuts, is correlated with taste disturbance (dysgeusia), also referred to as "pine mouth". Because of this known possibility of pine nut mixtures, a need was identified for a rapid streamlined assay to detect the presence of this species in the presence of other types of pine nuts. A locked nucleic acid probe was employed in a real-time polymerase chain reaction (RT-PCR) format to detect a single nucleotide polymorphism (SNP) unique to this species. This assay was able to detect P. armandii in homogenates down to ∼1% concentration (the lowest level tested) in the presence of several commonly co-occurring and closely related species of pine and should prove to be a useful tool for the detection of this species in food products.

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Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

Cited by 18 publications

References 26 publications

Plesiomonas shigelloides Revisited

Plesiomonas shigelloides Revisited

SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels

Development of a Locked Nucleic Acid Real-Time Polymerase Chain Reaction Assay for the Detection of Pinus armandii in Mixed Species Pine Nut Samples Associated with Dysgeusia

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